The effect of enzyme concentration‚ substrate concentration‚ pH‚ and temperature on the enzyme catalase. Introduction: Enzymes are biological catalysts; proteins and RNA. They are required for most biological reactions and they are highly specific. Each enzyme has an active site. The active site is the spot on the enzyme where a substrate fits in. Substrates binds with enzymes through the active site. Enzymes‚ being highly specific‚ only fit with one certain substrate. Enzymes and substrates
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Studying the pH of Strong Acid‚ Weak Acid‚ Salt‚ and Buffer Solutions The purpose of the current experiment was to determine the pH of various hydrochloric acid and acetic acid solutions‚ to determine the pH of various salt solutions‚ to prepare a buffer solution‚ and determine the effects of adding a strong acid and strong base to the buffer solution versus adding a strong acid and strong base to water. The measured pHs for the hydrochloric acid solutions were 1.6‚ 2.2‚ 2.9‚ and 3.8. The measured
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Title: “The Effect of Substrate Concentration‚ Enzyme Concentration‚ pH and Temperature on Enzyme Activity” Abstract: In the following experiments we will measure precise amounts of potato extract as well as Phenylthiourea‚ combined with or without deionized water and in some instances change the temperature and observe and record the reaction. We will also investigate the different levels of prepared pH on varying samples of the potato extract and the Phenylthiourea and record the results.
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Relationship of Chromium (Cr) mobility in soil and pH value using Electrochemical Method Siti Khadijah C.O. 1‚ Sabariah A.2‚ 3Zuliziana S.‚ Jestin Jelani4‚ Nordila A.5 1‚3‚4‚5 Lecturer‚ Civil Engineering Department‚ Faculty of Engineering‚ Universiti Pertahanan Nasional Malaysia 2 Lecturer‚ Faculty of Civil Engineering Universiti Teknologi Mara‚ Selangor‚ Malaysia Email : sitikhadijah@upnm.edu.my Abstract: Heavy metal contamination may provide a better indication of the potential risk
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Consulting Chemists Institute Introduction The Consulting Chemists Institute was contracted by a local food manufacturer to determine if sodium benzoate‚ a common food preservative‚ undergoes significant chemical changes at the pH level encountered in the acidic environment of the stomach. Experimental Method Sodium benzoate was prepared by addition of an equimolar amount of aqueous sodium hydroxide (3 M‚ ~ 20 ml) to a benzoic acid (1.256 g‚ 10.3 mmol) /water mixture (10 ml). Benzoic
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Interprofessional Studies. With reference to acid-base balance explore the role of the respiratory system in maintaining blood pH? ‘We live and die at the cellular level’ (Reid‚ 2011). Homeostasis is crucial for normal cellular function. Acid-base homeostasis is the part of human homeostasis and refers to the balance between the production and elimination of H+ hydrogen ions (pH) within the body fluids (William‚ Simpkins‚ 2001‚ p.236). Metabolic reactions within the cells often produce a huge excess
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monoprotic acids were determined with the aid of a buffer and pH meter. Experiment: This experiment was performed according to the procedures as provided in the Chemistry 111B laboratory manual: Po‚ Henry and Senozan‚ N. "General Chemistry Experiments Chemistry 111B"‚ 8th Ed.‚ 2004 Hayden-McNeil Publishing‚ pp 80-88. To determine the dissociation constants of weak monoprotic acids (HX and HY)‚ a buffer was needed to calibrate the pH meter. According to the manual‚ all four solutions to the experiments
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Investigating the Enzymatic Activity of Catecholase through Temperature‚ pH‚ Enzyme Concentration‚ and Substrate Concentration University of Alabama at Birmingham Burgess‚ B.N. Introduction: Background Enzymes are macromolecules that act as catalysts in living organisms by speeding up chemical reactions without being changed or destroyed by the reaction (Campbell and Reece‚ 2008). Enzymes are able to speed up the rate of a chemical reaction by decreasing the activation energy during the reaction
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During this experiment the change in pH of Citric acids is measured from start to equilibrium in mL. This experiment was tested by titration it had two separate trials. Through observations it was shown that the more concentrated acids needed more drops of NaOH to reach its equilibrium then the less concentrated acids. Introduction Sodium Hydroxide (NaOH) is a strong base and when it is titrated with a strong acid the equivalence point will be expected to be a pH of 7 (wyzant.com‚ Feb. 12). NaOH
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Research Question: To investigate how varying the pH of bromothymol blue affects the absorbance value of the solution which determines the equilibrium constant (pKa) of the indicator. Variables: Variables Variables Measured Method of measurement Independent pH of the six buffer solution A pH probe attached to a data-logger will be used to measure pH Dependent Absorption of the buffer solutions at wavelength 435.0nm and 617.0 nm A spectrophotometer (±0.001) will be used to measure absorbance
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