113L EXP# 7: Buffer Preparation and pH Measurements Revised . AMB 7-2005 Introduction: Even in quite dilute aqueous solutions‚ acetic acid is very slightly ionized (it would approach 99% ionization only as the concentration approaches 0.0 M): HC2H3O2(aq) + H2O(l) Ka = 1.8 x 10-5. H3O+ + C2H3O2- In general‚ if the acid is not extremely weak‚ the pH of a solution of a weak acid is governed by the concentration of the acid and Ka. Under similar conditions‚ the pH of a solution of a weak base is determined
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resists (or buffers) a change in its pH. That is‚ we can add a small amount of an acid or base to a buffer solution and the pH will change very little. How to calculate pH of buffer solution containing both acid and conjugate base? Dissociation constant definition 1.1 can be rearranged into or (note that due to sign change [A-] was moved to nominator). This is so called Henderson-Hasselbalch equation (or buffer equation). It can be used for pH calculation of solution containing
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Such factors include the amount of saccharide used and the type of saccharide‚ both of these factors will be put through a test in order to draw conclusions. The CO2 waste from the fermentation process will be examined‚ by measuring the pH of reaction‚ lower pH means more CO2 production. The first factor being measured is the type of sugar used in the fermentation of yeast. The types of sugar being used in the experiment is glucose‚ sucrose‚ and lactose. Glucose is a monosaccharide and is used
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pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer pH 7.0 buffer Incubation Cond. Boil‚ Inc. 37 deg.C 37 degrees C 37 degrees C 37 degrees C for 60 minutes IKI Test + - - + Benedict’s Test - ++ - - Chart 1 – Salivary Amylase Digestion of Starch (continued) Tube # 5 6 7 Additives DI Water‚ Maltose Amylase‚ Starch Amylase‚ Starch pH 7.0 buffer pH 2.0 buffer pH 9.0 buffer
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The pH of various mixtures and find the dissociation constant of acetic acid Chemistry (HL) Research Question: To determine the pH of various mixtures of sodium acetate and acetic acid in an aqueous solution and hence to find the dissociation constant of the acetic acid. Background: The mixture of sodium acetate and acetic acid in aqueous solution is a buffer solution. Buffer solutions are the solutions which resist a sudden change in the pH due to addition of small amounts of strong acid or base
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Lab 071-07 Dr. Roxanne Levandosky August 31‚ 2012 I. INTRODUCTION: A.PURPOSE: a. To define and understand the key terms. ~acid ~buffer system ~base ~acidosis ~pH ~alkalosis ~pH scale ~hypoventilation ~pH indicators ~hyperventilation b. To determine the acidic or base nature and actual pH of various substances. c. To discuss the formation of the carbonic acid/bicarbonate ion buffer system in maintaining homeostasis‚ especially with regard to ventilation. B
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Chemistry Resources: Harris ‘Quantitative Chemical Analysis’ Review: Pure water has a pH = 7 Autodissociation: H2O (( H3O+ + OH- K = [H3O+][OH-]/[H2O] -log[H3O+] = 7 [H3O+] = 10-7 M = [OH-] [H2O] = 55.56 M K = 1.8 x 10-16 ; pKa = 15.74 pKa is the acid dissociation constant; low pKa (strong acid‚ high pKa (weak acid we can also write Kw = [H3O+][OH-] Kw = 10-14 In water‚ pH + pOH = 14 pH scale Strong Acids: Complete dissociation
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The Effect of pH on the Rate of Enzyme Catalysis of Catalase Objectives: The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. More specifically‚ the objective was to perform an experiment in order to test the effect of pH on the function of the enzyme catalase. Introduction: Enzymes are proteins that act as catalysts for reactions. This simply means that enzymes lower the activation energy required
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2014 Zamir Deen Virtual Chem Lab 6-2 & 6-3 Ranking Salt Solutions by pH 6-2: Ranking Salt Solutions by pH In this assignment you will be asked to rank aqueous solutions of acids‚ bases‚ and salts in order of increasing pH. This is most easily done by first identifying the strong acids that have the lowest pH‚ the strong bases that have the highest pH‚ and the neutral solutions that have a pH near 7. The weak acids will have a pH between 1 and 6 and the weak bases between 8 and 14. The exact order
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Abstract: Introduction: Materials: * Chemicals: Buffer solution‚ pH 7.0‚ 50 mL Phenolphthalein indicator solution‚ 1.0 %‚ 1 mL Potassium hydrogen phthalate‚ KHC8H4O4‚ 2 g sodium hydroxide solution‚ NaOH‚ 0.1 M‚ 150 mL Unknown weak acid‚ 1.5g Water‚ distilled or deionized * Equipment: Balance Stir bar Beaker‚ 250mL Oven Buret‚ 50 mL pH sensor Desiccator Rising stand and buret clamp Erlenmeyer flask‚ 125mL Wash bottle with distilled water Funnel Weighing dishes‚ 2
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