enzyme concentration‚ temperature and pH on hydrolysis and solubilization of corn gluten as well as enzyme stability. The corn gluten was hydrolyzed by Alcalase enzyme (a bacterial protease produced by a selected strain of Bacillus licheniformis) that was chosen among five commercial enzymes examined. The optimum process conditions for hydrolysis and solubilization were obtained as 30 g L–1 substrate mass concentration‚ 2.5 mL L–1 enzyme concentration‚ 55 °C and pH 8. Under these conditions‚ the values
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your report) Activity: Enzyme Activity Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 60 °C (140 °F) 3. Sucrase activity decreases with increasing sucrose concentration. Materials and Methods Effect of pH on Enzyme Activity. 1. Dependent Variable. amount of product (glucose and fructose) produced 2. Independent Variable. pH 3. Controlled Variables. temperature; amount of substrate (sucrose) present; sucrase + sucrose incubation
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single sensor channel‚ and were difficult to assemble due to the use of discrete components. The measurement parameters consisted of temperature‚ pH or pressure‚ and the first attempts of conducting real-time noninvasive physiological measurements suffered from poor reliability‚ low sensitivity‚ and short lifetimes of the devices. The first successful pH gut profiles were achieved in 1972‚ with subsequent improvements in sensitivity and lifetime. Single-channel radio telemetry capsules have since
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Determining the equilibrium constant from pH values of changing concentrations of ethanoic acid Design Research question: How will altering the concentrations of ethanoic acid affect the pH value‚ and‚ in-turn‚ the equilibrium constant? Background information: When weak acids react‚ the reaction typically does not go to completion. Rather‚ the system goes to an intermediate state in which the rates of the forward and reverse reactions are equal. Such a system is said to be in chemical equilibrium
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Definition of Enzyme Unit and Specific Activity- 6 3.5 Determination of pH Optimum 6 3.6 Determination of Michaelis-Menten Constant 7 3.7 Ammonium Sulphate Fractionation 7 3.8 Gel Filtration on Sephadex 7 3.9 Chromatography on DEAE-cellulose 7 4 Results 8 4.1 Freshly Prepared Crude 8 4.2 Crude Thawed after a Week of Freezing 9 4.3 Sephadex 25 Gel Filtration 11 4.4 Determination of Optimum pH 12 4.5 Ion Exchange Chromatography 13 4.6 Michaelis-Menten Kinetics 13
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CROATICA CHEMICA ACTA CCACAA 78 (1) 99¿103 (2005) ISSN-0011-1643 CCA-2984 Original Scientific Paper Acid Value Determination in Vegetable Oils by Indirect Titration in Aqueous-alcohol Media Elena Kardash* and Yakov I. Tur’yan National Physical Laboratory of Israel (INPL)‚ Givat Ram‚ Jerusalem 91904‚ Israel RECEIVED JANUARY 16‚ 2004; REVISED JULY 21‚ 2004; ACCEPTED OCTOBER 28‚ 2004 Keywords acid value vegetable oils indirect titration A new method of acid value determination in
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Biology 20 IB Design Lab Does increasing or decreasing the ph level of a 3% concentration H2O2 (l) solution affect the amount of oxygen released between 15.0ml of the solution and a 5g sample of liver? The reaction is measured by the difference in mass of a balloon that is used to capture the amount of gas released by the catalase enzyme found in liver reacting with a basified 3% H2O2 (l) and an acidified 3% H2O2 (l) . Chemical reactions occur when two or more molecules interact and the molecules
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are true or not. Another fact which inspired me to do this project is that I am in touch with qualitative analysis whose knowledge with other factors helped me to do so. CONTENTS Ø Introduction Ø Theory Ø Apparatus Ø Chemicals required Ø Detection of pH Ø Test for Carbon Dioxide Ø Test for Glucose Ø Test for Phosphate Ø Test for Alcohol Ø Test for Sucrose Ø Result Ø Conclusion INTRODUCTION The era of cold drinks began in 1952 but the indianization of industry marked its beginning with launching of
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The Effect of the Type of Building Material on the pH of the Vinegar Introduction: For this experiment‚ the town council wanted to test which building material would be the best to build statues in a zoo. The building material with the lowest pH would be the best building material because while 7 is neutral anything below 7 is considered acidic and anything higher than 7 results in a base. Precipitation of 5.5 or lower is considered acid rain. Connecticut and most of the United States have to
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Determining the habitat preference of Artemia franciscana by creating artificial gradients of three abiotic factors: light‚ pH‚ and temperature Introduction In the study of ecology‚ species are often described as generalists if they live in broad environmental conditions or specialists if they live in narrow or specific environmental conditions (Reece et al. 2011). Artemia franciscana‚ the organism under study‚ is a well known generalist that has adapted it’s reproductive strategy to allow
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