and the equivalence point of 8.4‚ as well as the value of pH. Purpose: The purpose of the experiment was to utilize the techniques of titrations to measure the concentration of an acid or base in solution‚ to calculate molar mass of an unknown acid or base‚ and to determine the equilibrium constant of a weak acid or weak base. Materials: NaOH Unknown acid Distilled water Balance Erlenmeyer flask Funnel pH sensor or pH meter Ring stand and buret clamp 250-mL beaker 50-mL
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Collins Instructor: Catherine Rice Date: 07.09.2014 Predictions Sucrase will have the greatest activity at pH 5 Sucrase will have the greatest activity at 70 °C (158 °F) Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity Dependent Variable amount of product (glucose and fructose) produced Independent Variable pH Controlled Variables temperature‚ amount of substrate (sucrose) present‚ sucrase + sucrose incubation time
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Temperature‚ pH and oxygen requirement. Student: Temirlan Aitbekov Lab partner: Kanat Sadykov Instructor: Alessandra Clementi‚ MD‚GP Lab date: 7/11/14 Due date: 14/11/14 Nazarbayev University Abstract: This experiment is directed to investigate the various physical factors affecting the growth of microbes: temperature‚ pH of the
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Name: [Insert Your Name Here] Complete the following questions and submit your report. This document contains 4 pages. The total lab is worth 20 points. Purpose: The purpose of this lab is to demonstrate how temperature‚ substrate concentration‚ and pH can impact the rate of an enzyme reaction. In this experiment‚ you will use the Scientific Method to test your hypothesis. The enzyme used in this experiment is Invertase. This enzyme catalyzes the breakdown of sucrose (table sugar) into glucose and
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the amount of hydronium ion formed in water. Most often‚ it is expressed by pH‚ which equals the –log[H3O+]. The equilibrium constant for an acid is represented by Ka = [H3O+] [A-] / [HA]‚ where HA is the acid and A- is the dissociated acid. When comparing Ka’s‚ most commonly pKa is used‚ which is equal to –log Ka. In this experiment‚ the pKa of an unknown acid is determined by titrating it with NaOH and graphing its pH levels versus volume of NaOH titrated. The inflection point found by graphing
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mucopolysaccharides‚ but the specific group demonstrated is dependent on the dye solution pH. RECOMMENDED FIXATIVES 10% neutral buffered formalin and Bouin’s fixative. AVOID Chromate Fixatives MODE OF ACTION (1‚ 2‚ 3) Alcian blue binds electrostatically with the acidic groups in the mucopolysaccharide molecule. The components to be demonstrated are intensely stained if the dyer solution is used at the specific pH at which the reactive groups are fully ionized. To achieve full ionization of the
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is a solution of acetic acid‚ the solute‚ in water‚ the solvent. A buffer solution is one which resists changes in pH when small quantities of an acid or an alkali are added to it. An acidic buffer solution is simply one which has a pH less than 7. Acidic buffer solutions are commonly made from a weak acid and one of its salts often sodium salt. An alkaline buffer solution has a pH greater than 7. Alkaline buffer solutions are commonly made from a weak base and one of its salts. When hydrogen ions
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operations. Aluminum or iron salts can be used to for coagulation of particles and to form flocs that can settle out. Coagulation and flocculation tests provide the optimum dosages to remove turbidity and color‚ along with secondary effects such as adjusting pH. Jar tests also provide information on the amount of energy needed to provide the coagulation and flocculation treatments‚ as well as settleability of the flocs‚ and clarity of the water. They can also be used to study basic processes‚ for instance
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Grade 12 Biology (SBI 4U1) Nicole Mikulis Unit: Biochemistry Sept 14 2012 Lab: Effect of temperature and pH on catalase activity BACKGROUND Catalase is an enzyme that detoxifies chemicals that might harm the cell such as hydrogen peroxide (H2O2). The enzyme breaks H2O2 into water and oxygen. The production of the oxygen gas bubbles serves as evidence that the catalase enzyme is working. As catalase is breaking the bonds between H2O2‚ it is releasing energy in
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Publication No. 11137 Enzyme Activity Guided Inquiry Lab Turnip Peroxidase Introduction Peroxidase enzymes are widely distributed in plants and animals‚ including bacteria‚ to protect cells against the effects of oxidative stress and cell damage due to hydrogen peroxide. Peroxidases are easily extracted from turnips and other root vegetables and provide a model enzyme for studying enzyme activity—how the rate of an enzyme-catalyzed reaction depends on biotic and abiotic factors. Enzyme activity
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