DNA Aim This practical procedure allows you to amplify a 460 basepair fragment of DNA from within the control region of the mitochondrial genome. This can be done using three water baths or‚ if one is available‚ a thermal cycler (PCR machine). After it has been amplified‚ the DNA is run on an electrophoresis gel. Note: This method has been adapted from one developed by the Dolan DNA Learning Center at Cold Spring Harbor Laboratory. More details are available from the DNA Learning Center’s
Premium Polymerase chain reaction
Cheek cell practical Task 2 Risk assessment HAZARD RISK MITIGATION Methylene blue stains wear apron/gloves Swab stick spreading bacteria dispose correctly Microscope slide break/cut skin handle with care Cover slide break/cut skin handle with care Microscope drop/fall on foot position carefully
Premium Cell nucleus Bacteria Gene
Observations Control Measures Timescale Hazards and consequences Immediate and longer-term actions (Day/Wk/Month) 3. Hydraulic leak in Blowout Preventer (BOP) Accumulator identified during a pressure test prior to drilling out the shoe of the surface casing. The main hydraulic hose connected to the Annular Preventer is leaking noticeably where it is connected to the BOP. This could lead to the annular preventer failing during critical use when taking a kick and could
Premium Drilling rig
Week Three Practical Problem Questions A. Determine T(n) for n = 6‚ 7‚ & 8. T(6)=(2*6*10*14)/(6-1)!=14 T(7)= (2*6*10*14*18)/(7-1)!=42 T(8)= (2*6*10*14*18*22)/(8-1)!=132 B. Do you detect a pattern to these numbers? This pattern may arise out of the numbers or the manner in which you generated triangulations. (A closed-form function for T(n) is relatively straightforward‚ but is fairly nontrivial to construct; you will not have to explore that here.) The pattern for these numbers
Premium Group Reflection Change
Autopsies are vital to homicide investigations as they are the examination of a corpse by dissection and evaluation to determine the cause‚ mechanism‚ and manner of death. Geberth’s Practical Homicide Investigation does an excellent job in explaining these concepts. Cause of death is anything that is resulted in an individual to have died‚ which can be caused by an injury‚ illness‚ or anything that could cause harm to the body (Geberth‚ 2015‚ pg 815). Examples of causes of death are people dying
Premium Death Suicide Euthanasia
A2 BIOLOGY CORE PRACTICAL SUMMARY Name of practical Independent & dependent variables Observing patterns by Ecological sampling Random sampling Systemic sampling Other variables to be controlled Abiotic factors e.g. light‚ temperature‚ soil water‚ humidity‚ O2 concentration‚ pH‚ aspect‚ slope angle equipment Gridded Quadrat Tape measure Point quadrat Pitfall trap Sweep Net Pooter Tullgren funnel Baermann funnel Method and outcome Several methods. 1 random sampling = set up grid using
Premium DNA Oxygen
A Practical Guide to Sampling Statistical & Technical Team This guide is brought to you by the Statistical and Technical Team‚ who form part of the VFM Development Team. They are responsible for advice and guidance on quantative‚ analytical and technical issues. For further information about the matters raised in this guide‚ please contact: Alison Langham on ext. 7171 This guide is the latest in a series on sampling. It has been produced in response to a large number of requests
Free Sampling Sample size Sample
Practical Scientific Report This investigation shows the heart rate response from the resting heart rate‚ to mild exercise‚ moderate exercise and finally back to the resting heart rate again. Aim – This experiment involves two parties who partake in exercise and take each other’s heart rate responses several times at resting point and thereafter mild and moderate exercise. The aim is to check the difference in heart rate response from resting point and thereafter‚ physical activity. Hypothesis
Premium Harshad number Heart rate
of Woodworking Workshop and Offices Introduction Earlier today‚ I carried out a Health and Safety inspection of the above areas of our premises. The purpose of the inspection was two fold. First of all‚ to satisfy the requirements of the practical aspect of the NEBOSH General Certificate‚ and secondly to ensure that health and safety control measures in the above areas remained in force‚ and that we were complying with our statutory duties. Summary The inspection revealed that health
Premium Occupational safety and health
Practical Application A client comes to you for counseling. She states she is a Christian‚ in her third year of undergraduate school. She states she is struggling to choose a major because‚ “I am afraid that it may not be God’s will.” • What steps will you take to help her navigate this impasse? Why? • How might your personal position influence how you address this client’s concerns? Decisions come in all shapes and sizes‚ some are life threatening and others are simply insignificant. There are
Premium Bible