Physical and Chemical Reactions Lab #4 Alesa Cannon Objective: To investigate the physical and chemical properties of a pure chemical substance. Procedure: 1. Half-fill one well of a 24-well plate with 6 M HCl and half-fill a second well of the 24-well plate with 6 M NaOH. Suck up these chemicals into their labeled pipets for later use. 2. Perform the following steps on each of the substances to be tested. Complete all tests of one substance and record your observations before proceeding
Premium Chemistry Chemical reaction Hydrogen
It was originally hypothesised that the reaction time would decrease with higher concentrations of the reactants‚ potassium iodate and sodium bisulfite as collision theory stated that more molecules present in a given volume meant a greater chance they would collide. Furthermore‚ the time was expected to decrease with higher temperatures as it influenced the reactant particles by raising their kinetic energy. However‚ the data collected from this experiment only partially supported this hypothesis
Premium Chemical reaction Reaction rate Chemistry
The procedure was taken from “From Drosophila cDNA in E. coli plasmid to homologous human proteins” lab manual (4). - Colony Picking: Two E. coli colonies were grown on agar plates and treated with ampicillin. They contained the plasmid with genes for ampicillin resistance and Drosophila cDNA sequence. - Plasmid Isolation: We used the QuickLyse Miniprep Plasmid DNA purification systems to isolate the plasmid DNA. Indeed‚ the bacterial cells were removed from the liquid broth and were resuspended
Premium DNA Gene Molecular biology
ANALYSIS OF GROUP III CATIONS Precipitation of Group III Cations and Separation into Subgroups Analysis of Subgroup A of Group III Cations Analysis of Subgroup B of Group III Cations (PART 1) Analysis of Subgroup B of Group III Cations (PART 2) ----------------------- Use decantate from group II cations Precipitate Decantate Consider as Group IIIB. Consider as Group IIIA. Centrifuge and separate. Add 10 drops of 1M HCl. Wash with few drops of H2O. Precipitate
Premium Hydrogen Ammonia Solubility
The purpose of this lab was to find out the reaction of iron nails and CuSO4‚ copper sulfate solution. Before starting the experiment‚ there were two possible outcomes of the reactions. CuSO4 + Fe → FeSO4 + Cu or 3CuSo4 + 2Fe → Fe2(SO4)3 + 3Cu. The ion chart showed that iron can only make two charges‚ +2 and +3. As the liquid evaporated‚ the weight changed because the liquid added to the nail’s weight. In a real world situation‚ scientists can use ratios to determine how much of a substance that
Premium Chemistry Chemical reaction Copper
DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal
Premium DNA Bacteria Gene
Constant of an Unknown Ester Hydrolysis Reaction Abstract The experiments to follow determined that the equilibrium concentrations of the reaction: ester + water ↔ alcohol + acid‚ are equal to 0.0363 moles of ester‚ 0.2852 moles of water‚ and 0.0268 moles each of alcohol and acid. Using this information the equilibrium constant was determined to be 0.06938. 1. Introduction In this lab the equilibrium constant‚ Kc‚ for the acid catalyzed reaction between an unknown ester and water
Premium Chemistry Chemical equilibrium Chemical reaction
The hypothesis If a chemical reaction occurs‚ then there will be indicators of a chemical change‚ was supported throughout this experiment. Throughout this experiment there were several reactions that took place‚ and an indicator of such chemical reactions could be seen throughout each one. The first piece of identifiable evidence was present in the first step‚ which was when copper (II) nitrate and sodium hydroxide were combined. When stirred‚ a blue precipitate formed at the bottom of the test
Premium Chemical reaction Chemistry
was synthesis by reacting concentrated hydrochloric acid using nucleophilic substitution sn1. In the substitution reaction the Hydrogen in HCl protonated the alcohol group in t-pentyl alcohol and turned it to a good leaving group (H2O). a tertiary carbon cation was formed. a nucleophilic attach of the negative Cl- attacked the carbon cation forming t-pentyl chloride [7]. As the reaction proceeded‚ extraction and distillation techniques were used to get a pure product of t-pentyl chloride. The percent
Premium Chemistry Oxygen Chemical reaction
experiment the objective was to perform a Diels-Alder reaction using cyclopentadiene and maleic anhydride to synthesize the product‚ cis-Norbornene-5‚6-endo-dicarboxylic anhydride. The Diels-Alder reaction is one of the most important reactions in organic chemistry and was first investigated by Otto Diels and Kurt Alder in Germany. It is a [4+2] concerted cycloaddition reaction which involves a diene and a dienophile. The Diel-Alder reaction are mainly used for creating new carbon-carbon bonds‚ mostly
Premium Carbon Oxygen Chemistry