CHEMICAL REACTIONS: ACID-BASE BUFFERS Short Overview Acids and bases represent two of the most common classes of compounds. Many studies have been done on these compounds‚ and their reactions are very important. Perhaps the most important reaction is the one in which an acid and base are combined‚ resulting in the formation of water (in aqueous solution) and a salt; this reaction is called neutralization. A buffer solution is a solution that contains both an acid and a salt containing
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Purpose: To find out if the Unknown substances are bases or acids. Materials: goggles aprons paper for data table graduated cylinder unknown I substance beakers/test tubes stirring rod litmus paper pH indicator paper pH color chart phenolphthalein scoopula eye-dropper digital pH meter magnesium ribbon calculator unknown II substance forceps sodium bicarbonate Procedures: Test the pH of the Unknown substances with litmus paper and pH indicator paper and match to color chart
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fermented from sugar. The fermentation of ethanol results in the production of acetic acid (CH3COOH). The typical pH of vinegar ranges anywhere from 2 to 3.5‚ although shop-bought vinegar usually measures 2.4 www.wisegeek.com/what-is-vinegar.htm fig 1 In this study we will determine the amount of acid in a vinegar sample by using titration‚ a common technique in chemistry a typical shop brought vinegar has an acid percentage of 5% see fig 1. A titration is a technique where a solution of known
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The acid-fast stain is performed on samples to demonstrate the characteristic of acid fastness in certain bacteria. Acid fastness is a characteristic that is shared by just a few organisms‚ so staining to determine if organisms possess this trait is useful in microbial identification schemes. The Ziehl-Neelsen method has endured as a reliable and effective way to demonstrate the acid-fast bacteria. Materials: 18-20 nutrient hour agar slant culture of Staphyloccus aureus 4 day old nutrient
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Abstract The objective of this experiment was to observe multi-step purification of benzoic acid after performing the extraction from a mixture containing benzoic acid‚ cellulose‚ and methyl orange. Recrystallization was done to remove impurities from the sample. The amount of BA recovered during recrystallization is much less than the extracted amount of BA. The difference between the pure and impure samples was observed by comparison of melting points. It was found that impure samples will have
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will show a colour change. After that record the initial reading of the burette and making sure that all your burette readings must be to an appropriate precision. Titrate the contents of the conical flask by adding to it 0.100 mole dm3 hydrochloric acid from the burette. Add the solution slowly. Swire the flask gently to mix the contents at the end indicator turns as define red colour. This is the end point of the titration. Record the final burette reading in your table of result. Repeat the titration
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Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired analyte
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Acid and Base Titrations: Preparing Standardized Solutions Introduction: This experiment focuses on titrations of acids and bases. A titration depends on addition of a known volume of solution and is a type of volumetric analysis. Many titrations involve either acid-base reactions or oxidation-reduction reactions. In this experiment we do one of each. We monitor the pH of the reaction with the use of a color indicator. We also learn about the standardization of bases (NaOH) and acids (HCl) which
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Rain Man is a movie that demonstrates the life of two men who live very different lives; however‚ learn to live together during their journey. A selfish man‚ named Charlie‚ is forced to slow down his life when he meets the brother that he never knew existed. His brother‚ Raymond‚ is an autistic savant and just inherited three million dollars. Three million dollars that Charlie felt he was entitled to‚ was left to his brother whom had no concept of money so he wanted it for himself. The brothers embarked
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Alanine (ala‚ A) (neutral‚ non-polar) Arginine (arg‚ R) (basic‚ polar) Asparagine (asn‚ N) (neutral‚ polar) Aspartic Acid (asp‚ D) (acidic‚ polar) Cysteine (cys‚ C) (neutral‚ slightly polar) Glutamic Acid (glu‚ E) (acidic‚ polar) Glutamine (gln‚ Q) (neutral‚ polar) Glycine (gly‚ G) (neutral‚ non-polar) Histidine (his‚ H) (basic‚ polar) Isoleucine (ile‚ I) (neutral‚ non-polar) Leucine (leu‚ L) (neutral‚ non-polar) Lysine (lys‚ K) (basic‚ polar) Methionine (met‚
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