AN ASSIGNMENT ON CELL CULTURE [pic] ASSIGNED BY | | | |Dr. Md. Bahanur Rahman |Dr. Sukumar Saha | |Professor |Associate Professor | |Dept. of Microbiology and Hygiene
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INTRODUCTION An important aspect of any biotechnological processes is the culture of animal cells in artificial media. These animal cells in culture are used in recombinant DNA technology‚ genetic manipulations and in a variety of industrial processes. Now-a -days it has become possible to use the cell and tissue culture in the areas of research which have a potential for economic value and commercialization. The animal cell cultures are being extensively used in production of vaccines‚ monoclonal antibodies
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Anchorage Dependent Animal Cell Culture Animal Cell Culture: Its origin and importance Prior to discussing about the anchorage dependence of animal cell cultures‚ let us first briefly discuss animal cell culture in general and its importance. Animal cell culture has a history of over 100 years when the first attempts to grow cells were made by Ross Harrison who was able to show the development of nerve fibres from frog embryo tissue cultured in a blood clot. Physiologist Alexis Carrel was able
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ATCC® PRIMARY CELL Culture Guide tips and techniques for culturing primary cells The Essentials of Life Science Research Globally Delivered™ Table of Contents ATCC® Primary Cell Solutions®...........................................................................1 ATCC Primary Human Endothelial Cell Solutions.............................................5 ATCC Primary Human Smooth Muscle Cell Solutions.......................................9 ATCC Primary Human Epithelial Cell Solutions........
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Applications of cell culture Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and other products of biotechnology Biological products produced by recombinant DNA (rDNA) technology in animal cell cultures include enzymes‚ synthetic hormones‚ immunobiologicals (monoclonal antibodies‚ interleukins‚ lymphokines)‚ and anticancer agents. Although many simpler proteins can be produced using rDNA in bacterial cultures‚ more complex proteins that are glycosylated (carbohydrate-modified)
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Stem Cell Culture Human embryonic stem cells are pluripotent stem cells derived from the inner cell mass of the blastocyst‚ an early-stage embryo. To culture these stem cells under controlled environments‚ one first must have a growth medium. This growth medium is made with DMEM/F12‚ which is cell culture medium‚ a 20 percent Knockout Serum Replacement‚ 100 μM β-Mercaptoethanol‚ 1 percent non-essential Amino Acid‚ and a Basic Fibroblast Growth Factor. This medium is then changed out everyday
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Cell Biology Lab - Homework 1 Due to the week of Oct. 6-10th This is NOT a lab group project. Do the work individually. You may use any source other than your fellow students. Show your work‚ use your own words and cite all references. 1. (20 points) The HeLa S3 cancer cell line is considered to be “continuous” due to acquired mutations that enable the cells to evade senescence. This property is one of several hallmark characteristics of cancer cell lines. Describe three hallmark characteristics
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Cell Biology Lab - Homework # Due the week of Feb 3rd This is NOT a lab group project. Do the work individually. You may use any source other than your fellow students. Show your work‚ use your own words and cite all references. 1. (14 points) The HeLa S3 cancer cell line is considered to be “continuous” due to acquired mutations that enable the cells to evade senescence. This property is one of several hallmark characteristics of cancer cell lines. Describe three hallmark characteristics
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Cells‚ Cell Division‚ and Cell Specialization Fundamentally Different Types of Cell Prokaryotic Cell- single celled: only DNA+ structure (“before nucleus”) E.g. zygote-complete DNA Eukaryotic Cell-multi-celled (“after nucleus”) Prokaryotes Eukaryotes DNA In “nucleoid” region Within membrane-bound nucleus Chromosomes Single‚ circular Multiple‚ linear Organelles None Membrane-bound organelles Size Usually smaller Usually larger- 50 times Organization Usually single-celled Often multicellular
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Henrietta Lacks‚ but scientists know her as HeLa. She was a poor Southern tobacco farmer who worked the same land as her slave ancestors‚ yet her cells- taken without knowledge- become one of the most important tools in medicine (Rebecca Skloot). According to the scientists who have been growing HeLa for countless experiments‚ if you could pile HeLa cells ever grown onto a scale‚ they’d weigh more than 50 million metric tons- as much as Empire State Buildings (The NY Times). Long times ago‚ even
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