An Introduction to DNA microarrays Rebecca Fry‚ Ph.D. http://www.buffalo.edu/UBT/UBT What is a DNA Microarray? genes or gene fragments attached to a substrate (glass) Tens of thousands of spots Hybridized slide Two dyes Image analyzed 1 The Beginnings of Microarray Technology Lockhart et al.‚ 1996 Nature Biotechnology “Expression monitoring by hybridisation to high-density oligonucleotide arrays” Schena et al.‚ 1995 Science “Quantitative monitoring of gene expression
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Cells‚ Cell Division‚ and Cell Specialization Fundamentally Different Types of Cell Prokaryotic Cell- single celled: only DNA+ structure (“before nucleus”) E.g. zygote-complete DNA Eukaryotic Cell-multi-celled (“after nucleus”) Prokaryotes Eukaryotes DNA In “nucleoid” region Within membrane-bound nucleus Chromosomes Single‚ circular Multiple‚ linear Organelles None Membrane-bound organelles Size Usually smaller Usually larger- 50 times Organization Usually single-celled Often multicellular
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Leigh Ann Santana DNA Extraction Lab DNA extraction is an important process because the DNA first needs to be purified away from proteins and other cellular contaminants. Cell are needed‚ because that is where the DNA is located. Inside almost every cell in our bodies is a nucleus‚ and inside each nucleus is about two meters of DNA. The following steps are needed to purify DNA from a cheek swab. Collect cheek cells‚ Burst cells open to release DNA‚ separate DNA from proteins and debris‚ and isolate
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DNA Assignment Keshia Yeates BIOLOGY 4U 1. DNA molecule with a length of 2m can fit inside a nucleus that is invisible by the naked eye by coiling around itself several times. It continues to coil even more (Supercoiling)‚ arranging itself on histones‚ which are proteins. 2. Nitrogen bases determine the traits of living organisms by coding the 20 amino acids. The order of the amino acids determines what protein is made; therefore‚ proteins are the makeup of all living organisms. Proteins regulate
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Study guide Exam 1 Ch 1-5 CH 1 Overview‚ what is evolution‚ what is biology‚ what is life Evolution scientific explanation for: the unity and diversity we see in various organisms the suitability of organisms to their environments is evolution Hierarchy – molecules‚ cells‚ tissue‚ organ‚ organisms‚ populations‚ communities‚ ecosystem‚ biosphere Emergent properties – zooming from molecular level out to see function Reductionism (complex to simple) and Systems Biology (dynamic) Organisms
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automatic recall of multiplication and related division facts to be prepared for the study of higher lessons‚ in which solving multi-step equations is a fundamental task. Prior to these suggestions are the uses of multiplication and division of math-facts. Math-facts are small number or single digit combination of the four operations. Automaticity on the other hand is speedy recall and accurate answers to math-facts like multiplication and division. To fully understand math-fact automaticity‚ Boso
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EXAM-1 STUDY GUIDE DUE ON FEBRUARY 28TH PLEASE SUBMIT IN THE DROP BOX IN MICROSOFT WORD. CHAPTER-1 The Microbial World and You List several ways in which microbes affect our lives. Help maintain the balance of living organisms and chemicals in our environment. Marine and fresh water microorganisms form the bases of the food chain in oceans‚ lakes‚ and ricers. Soil microbes help break down wastes and incorporate nitrogen gas from the air into organic compounds‚ thereby recycling chemical
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DNA EXTRACTION In extracting chromatin from the cells of wheat germ there are seven steps to follow. The optimal cell to use would be the polyploidal eukaryotic. Eukaryotes have nucleus membrane-bound organelles‚ while prokaryotic does not. The polyploidal eukaryotic cell has DNA that is held in the nucleus while the prokaryote has DNA that floats freely around the cell. The DNA of eukaryotes is more complex and extensive than the other. Prokaryote is a bacterial cell that
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AP Psych Unit 4 Practice Test Multiple Choice Identify the choice that best completes the statement or answers the question. ____1.The process of receiving and representing stimulus energies by the nervous system is called a. priming. b. synaesthesia. c. accommodation. d. sensation. e. perception. ____2.The process by which we select‚ organize‚ and interpret sensory information in order to recognize meaningful objects and events is called a. sensory adaptation. b. parallel processing.
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DNA Replication DNA replication is a biological process that occurs in all living organisms and copies their DNA. The initiation of DNA replication starts with two steps. First an initiator protein unwinds a short stretch of the DNA double helix. Then a protein called helicase attaches to and breaks apart the hydrogen bonds between the bases on the DNA strands‚ pulling apart the two strands. DNA replication starts when one double-stranded DNA molecule produces two identical copies of the molecule
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