Cell Fractionation: Isolation of Mitochondria from Cauliflower and Determination of Specific Enzyme Kinetics Introduction Mitochondria is an organelle found in eukaryotic cells that play a role in biochemical processes such as respiration and energy production. Mitochondria even play an important role in apoptosis‚ or programmed cell death. This is achieved by disruption of electron transport‚ oxidative phosphorylation‚ and ATP production or even the release of proteins that trigger activation
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Cell Fractionation * Cell Fractionation: takes cells apart and separates the major organelles and other subcellular structures from one another. * Tissue cells are the first one to be homogenate or broken apart. * Plasma membranes are broken up so that there internal contents spill out and mix together and this is called homogenate. * Homogenate is in spun in a higher rate of speed in a process called centrifugation. And that speed can vary that why it’s called differential
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Cell Fractionation i) EQUIPMENT: Cold Liver‚ Blender‚ Gauze‚ Fridge‚ Isotonic Solution‚ Centrifuge‚ Tubes‚ Scalpel‚ HOMOGENIZATION • Take chilled liver tissue and cut into smaller pieces. • Place liver into blender with cold isotonic solution. • Blend until you have a thick soup like substance (homogenate). • Filter the mixture to remove large debris through gauze into a tube. DIFFERENTIAL CENTRIFUGATION • Attach tubes of homogenate to the refrigerated Centrifuge and turn on to spin
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NAME: Aleema Chelsea Chinchamee LAB PARTNERS: Karishma Ramrattan‚ Vishma Ramsumair and Sharona Badree ID #: 814003081 DATE (of lab session): Tuesday 24th March‚ 2015 DEMONSTRATOR: Maurissa Course Code & Title: BIOL 1362- Biochemistry I Title of Lab: Investigating Enzymatic Activity in Sweet Potato‚ Irish Potato Extract and Milk. Aims: 1. To determine the effect of ascorbic acid on Polyphenol Oxidase (Phenolase). 2. To determine the level of specificity of Phenolase using the
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Biochemistry Eukaryotic Cells (animal cells) * Nucleus * Organelle Mitochondria: “power house” Where energy (ATP) produced and stored Prokaryotic Cells (plant cells) * Cell wall * Chlorophyll * Cytoplasts: where photosynthesis occurs General Equations:- Photosynthesis: * 6CO2(g) + 6H20(l) +E(solar) -> C6H1206(glucose) + 602(g) Respiration: * C6H1206 + 602(g) -> 6CO2(g) + 6H20(l) + E Metabolism: * Thyroxin: A hormone that controls the bodies
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Bhatia 1 Ravnit Kaur-‐Bhatia Biochemistry 444 Term Paper Dr. Cristina Clement Date: December 16th‚ 2012 My Protein Data Bank (PDB) entry code is 1TW4 and this structure was determined by crystallography. The primary paper describing this paper is: Title: Crystal structure of chicken liver basic
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Section: 1246 T/Thr 5:00-9:20pm Textbooks: Principles of Anatomy and Physiology 13th ed.‚ By Tortora and Derrickson; John Wiley‚ Publisher‚ older or newer editions are acceptable Anatomy and Physiology 101 Lab Manual‚ By Sterling Roulette and Cheri Hodge Other items required: 1) Lab apron or old oversized shirt 2) "Scantron" computer answer cards #100 (please have ready for the first test not the quiz) 3) Colored pencil set 4) Latex gloves Catalog description: Anatomy and Physiology 101:
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Biochemistry‚ sometimes called biological chemistry‚ is the study of chemical processes within‚ and relating to‚ living organisms.[1] By controlling information flow through biochemical signaling and the flow of chemical energy through metabolism‚ biochemical processes give rise to the complexity of life. Over the last 40 years biochemistry has become so successful at explaining living processes that now almost all areas of the life sciences from botany to medicine are engaged in biochemical research
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Biochemistry- The Basics An Atom is the basic unit of a chemical element ( smallest unit for measuring chemical & physical properties) Smallest particle of an element is an atom A Particle is a small portion of matter Matter is composed of elements___ Matter makes up everything in the universe. An Element is a pure substance that cannot be broken down into a simpler substance. ex. carbon‚ hydrogen‚ etc A molecule is a group of atoms bounded together ex O2 A chemical compound
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from the simple elements and compounds on the Earth’s surface. Biochemistry asks how the remarkable properties of living organisms arise from the thousands of differ-ent lifeless biomolecules. When these molecules are iso-lated and examined individually‚ they conform to all the physical and chemical laws that describe the behavior of inanimate matter—as do all the processes occurring in living organisms. The study of biochemistry shows how the collections of inanimate molecules that consti-tute
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