and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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The Effect of Temperature on Beet Cell Membranes and Concentration of Betacyanin Released Introduction Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell et al.‚ 2008). Each membrane layer is composed of a phospholipid layer‚ which is semi-permeable and possesses the ability to control the movement of diffusion. Within the centre of a cell‚ the vacuole that is responsible for storage and
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“Cell Transport Lab” Hypothesis: I hypothesis that there will be a movement of molecules out of the egg‚ and will move into the water solution. Osmosis will occur within the egg‚ and in the process the molecules of the egg will diffuse. Time (hrs) | Observations | Circumference (cm) | * 0 | * Egg is of normal weight and size * No membrane is being shown‚ and is almost perfect | * 14 centimeters | * 24 | * Egg has gotten larger taking in some of the water * Shell is soft
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Brianna Cooper Brandon Boucher 2/16/12 Purpose: To understand how both voltaic and electrolytic cells function. Procedure: Refer to pages 34-36 of laboratory notebook for experimental details Results and Discussion: Voltaic Cell Reactions | | | Overall Cell Reaction | Observed Voltage | Theoretical Voltage | Cu2+(aq)+Zn(s) Cu(s)+Zn2+(aq) | 0.947 V | 1.10 V | Cu2+(aq)+Sn(s)Cu(s)+Sn2+(aq) | 0.571 V | 0.473 V | Cu2+(aq)+Fe(s) Cu(s)+Fe2+(aq) | 0.512 V | 0.777 V | Cu2+(aq)+Mg(s) Cu(s)+Mg2+(aq)
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BioLab3 Lab Report 7 Cellular Respiration Answer Key Student Name: I. The ATP Cycle Define the following terms: Autotroph Heterotroph Aerobic respiration Anaerobic respiration Complete the chart below: Letter Defining Term A B C D II. Anaerobic Respiration Define the following terms: Alcoholic fermentation EXERCISE 1 – Alcoholic fermentation At intervals of 20‚ 40‚ and 60 minutes‚ the tubes are removed. Record the
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Cell Surface Glycoprotein Receptor Analysis Using Concanavalin A Purpose: During this experiment we compared the hemagglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample‚ and determine through observations
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CELL REPRODUCTION DNA is the cell’s genetic material; chromosomes are the carriers of this genetic information. In proka-ryotes‚ the chromosome is a single circle of DNA. In eukaryotes‚ each chromosome is a complex of DNA and histone proteins found in the nucleus. BINARY FISSION Prokaryotic cells reproduce via binary fission. In this process‚ DNA Is replicated‚ and the cell splits in two roughly equal parts‚ each with a copy of the cell’s DNA. EUKARYOTIC CELL CYCLE Eukaryotic cells reproduce
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principle‚ a purple onion cell is going to be bathed in different concentrations of salt (NaCl) water. Problem: How do solutions of various salt concentrations influence osmosis in relation to an onion cell? Hypothesis What will happen to the onion cell if it is placed in salt water? What will happen to the onion cell if it is placed in distilled water? If ..the onion cell is placed in salt water then... the cell will shrivel up The
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