Abstract: In this Lab we used the chemical DPIP to detect the rate of succinate broken down by the mitochondrial solution. We detected the amount of DPIP in the solution with a spectrophotometer and measuring the absorbance of light at the 600nm range. DPIP is a useful chemical to use in this experiment because it goes from a blue color when oxidized to a colorless liquid (Ogura‚ 281)‚ this is due to the hydrogen ions and electrons released during the transitional step between succinate and fumarate
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Formal Lab Report #1 I. Basics Title of the Experiment: The Empirical Formula of an Oxide Authors: Section Number: Chemistry Location and Date II. Abstract The aim in this lab was to determine the empirical formula of an oxide of magnesium through combustion in air. This was achieved by heating an established mass of magnesium in air inside of a crucible‚ ultimately attaining a compound that contained Mg and O. The major result of the experiment was the empirical formula of the oxide of magnesium
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IB Chemistry Experiment 4 Composition of a Post-1983 Penny Lab Report Background: Through the use of percent composition one can easily find out how much of a certain element is in a compound or an item such as a post-1983 penny. The experiment will be using HCl a strong acid to dissolve all the zinc inside the outer copper shell. The reaction follows as such; Zn+2HCLZnCl2+H2‚ but there will be no reaction with copper. The portion that will be measured is the mass of copper left after all of
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Introduction Microscopy is an essential technique not only in cell biology but natural science as a whole. We compared different types of microscopic techniques ‚ according to the specimen used and the scope of the experiment. Two specimens‚ stained and unstained‚ containing CHO cells‚ were prepared‚ examined and analyzed under the microscope using bright field (HF)‚ dark field (DF) and phase contrast (PH) settings. In addition‚ the four phases of cell division cycle were estimated.. Bright field microscopy
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Effects of Temperature on the Formation of Cheese Curds This lab was performed in order to find what temperature the enzyme chymosin is most effective at forming cheese curds. An enzyme is a protein that acts as a catalyst to speed up biological reactions in cells. They work by lowering the activation energy and help to orient the molecules correctly. Chymosin was originally taken from the stomachs of calves‚ and was called rennet‚ and then purified to make rennin. Eventually to catch up with
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absorbance‚ which was used to calculate initial cell density. The caps on each of the samples were then loosened to allow movement of gas in and out of the tube. After a week of growth under fluorescent lighting‚ the tubes were inverted for mixing and measured for absorbance again. The values were used to calculated the corresponding final cell density. The calculations of initial and final density of cells were used to determine the algal growth rate of cells per milliliter per
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Antibiotic Lab Report By: Sierra Toll Hour: 6 Title: The effects of antibiotics on the growth of Escherichia Coli Problem: How do antibiotics affect the growth of E coli? Hypothesis: If Penicillin is applied to the bacteria‚ then the zone of inhibition will be larger. Background: The purpose of this lab was to see how effective a certain antibiotic is to kill E coli. The lab tested three antibiotics‚ Penicillin‚ Erythromycin‚ and Neomycin. The zone of inhibition‚ which is the amount of space
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Coli bacteria. We began the lab by first obtaining two sterile microcentrifuge tubes‚ one was label “GFP+” while the other one was “GFP–” because this one served as a control meaning nothing goes inside this one. With gloves on 25 microliters competent cells‚ which is e. Coli bacteria compete in calcium chloride and heat shock was added to each of the microcentrifuges. Eventually‚ both of the
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General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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Receptors and Ligands: Examining the effects of pharmaceutical compounds on Daphnia magna physiology Abstract The following study was conducted in order to determine the effects of four different pharmaceutical compounds on the heart rate of Daphnia specimens. After basal heart rate was observed and computed‚ four different solutions each containing either an adrenergic‚ muscarinic‚ or nicotinic agonist or antagonist was tested against individual Daphnia. Observations were made through a compound
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