Cell Fractionation: Isolation of Mitochondria from Cauliflower and Determination of Specific Enzyme Kinetics Introduction Mitochondria is an organelle found in eukaryotic cells that play a role in biochemical processes such as respiration and energy production. Mitochondria even play an important role in apoptosis‚ or programmed cell death. This is achieved by disruption of electron transport‚ oxidative phosphorylation‚ and ATP production or even the release of proteins that trigger activation
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Cell Fractionation * Cell Fractionation: takes cells apart and separates the major organelles and other subcellular structures from one another. * Tissue cells are the first one to be homogenate or broken apart. * Plasma membranes are broken up so that there internal contents spill out and mix together and this is called homogenate. * Homogenate is in spun in a higher rate of speed in a process called centrifugation. And that speed can vary that why it’s called differential
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Cell Fractionation i) EQUIPMENT: Cold Liver‚ Blender‚ Gauze‚ Fridge‚ Isotonic Solution‚ Centrifuge‚ Tubes‚ Scalpel‚ HOMOGENIZATION • Take chilled liver tissue and cut into smaller pieces. • Place liver into blender with cold isotonic solution. • Blend until you have a thick soup like substance (homogenate). • Filter the mixture to remove large debris through gauze into a tube. DIFFERENTIAL CENTRIFUGATION • Attach tubes of homogenate to the refrigerated Centrifuge and turn on to spin
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Cheek cell practical Task 2 Risk assessment HAZARD RISK MITIGATION Methylene blue stains wear apron/gloves Swab stick spreading bacteria dispose correctly Microscope slide break/cut skin handle with care Cover slide break/cut skin handle with care Microscope drop/fall on foot position carefully
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Result: Part A: Table 1: Fractionation of amino acid Fraction No | Response to Ninhydrin test(level of blue colour change) | Response to Sakaguchi test (colour change) | 1 | X | yellow | 2 | Dark blue | yellow | 3 | Dark blue | Pale orange | 4 | Blue | Pale orange | 5 | Pale blue | Pale orange | 6 | Blue | Pale orange | 7 | Pale blue | Pale orange | Discussion Part A In the separation and purification of a single protein or amino acids‚ a solution containing the desired
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To Investigate the Effect of Varying pH on the Rate of a Reaction Catalysed by Trypsin Aims I hope to determine the effect of varying pH on the rate of reaction catalysed/controlled by the enzyme trypsin. Method 1. Add 4cm³ of the 2% trypsin solution in to 6 test tubes labelled A-F 2. Add 4cm³ of the appropriate buffer solution to each. ( I will be testing pH 4‚5‚7‚8‚8.8 and 10) 3. Add 4cm³ of distilled water and 4cm³ of the appropriate buffer solution to 6 control test tubes labelled CA-CF to see
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cholera‚ syphilis‚ anthrax and leprosy. The most common fatal bacterial diseases are respiratory infections such as tuberculosis (Barnum S.R; 1998). Nucleic acids encode information relating to cell structure and function. Cells have the ability to make copies of their DNA and pass this information to daughter cells. Nucleic acids are polymers of nucleotides. Nucleotides are composed of ribose (a 5` carbon) sugar and either a purine and pyrimidine base at 1` position. The
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Student Laboratory Guide Write-up: Comprehensive History With your “patient” assuming the role of a client‚ conduct a comprehensive history. History Biographic Data Date: 10/04/2013 Initials only: L.C. Gender: M Race: H Date of Birth: 04/15/1972 Age: 42 Marital Status: married Historian quality : Good Occupation: Aircraft mechanic Source of Data: Patient Reason for Seeking Care/Presenting Problem: Present Health Status Anemia Asthma Artery Disease Osteoarthritis
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CONSERVATION OF MOMENTUM PRACTICAL WRITE UP AIM: To investigate if momentum is conserved in two-dimensional interactions within an isolated system. HYPOTHESIS: Without the effects of friction the momentum will be conserved in the isolated system. In all three experiments the momentum before the interaction will equal the momentum after the interaction. METHOD: An air hockey table was set up and a video camera on a tripod was placed over the air hockey table. The camera was positioned so it was
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Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain. In plant and animal cells‚ similarities and differences exist because of varied life functions
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