and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Osmosis and Diffusion During the past few weeks in my Ag Biology class we have been learning about and reviewing both osmosis and diffusion. We did two different labs‚ one on osmosis; the other on diffusion. The first lab we did was our diffusion lab. We learned that diffusion is moving something from an area of high concentration to a low concentration. We were showed that Mio and other flavor drops perform diffusion when put in water as an example. For the lab part we put a mixture of starch
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Testing Cell Transportation Across a Membrane Introduction Cells have the amazing ability to transport certain molecules in or out of their membrane. Some require no energy to do so (passive transport) while others require energy to be processed through (active transport). There is also the transportation of water across a membrane‚ which has its own term of osmosis. Too much of something can be taken in‚ or too little enters. This especially happens to plants‚ who require water (and sun) to live
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Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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Simple diffusion The term simple diffusion refers to a process whereby a substance passes through a membrane without the aid of an intermediary such as a integral membrane protein. The force that drives the substance from one side of the membrane to the other is the force of diffusion. In order for substances to pass through a cell membrane by simple diffusion it must penetrate the hydrophobic core of the phospholipid bilayer. The types of molecules that can do this are themselves substantially
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Simple diffusion and facilitated diffusion both _______. move solutes with their concentration gradient Which of the following would decrease the rate of facilitated diffusion? decreasing the number of carrier proteins What happens to facilitated diffusion when the protein carriers become saturated? The maximum rate of transport will occur. What happened when sodium chloride was added as a solute in the left beaker? There was no change in the transport rate of glucose. Which of the following
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The purpose of this lab was to hypothesize about membrane traffic in lab‚ explain the differences between the solutions hypertonic‚ hypotonic‚ and isotonic and how they respond using the understanding of the cell membrane structure‚ types of transport mechanisms such as active‚ passive‚ diffusion‚ osmosis‚ and explain the movement of particles moving across the cell membrane. In this lab was divided into two parts. The first part was varying the concentration and the second part was varying the temperature
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Diffusion and Osmosis in an egg Low concentration 1.1 Diffusion is the process of molecules spreading from areas of high concentration to areas of low concentration. High concentration 1.1 Osmosis is the diffusion of water molecules through a semi-permeable membrane from the area of low concentration of solute to the area of high concentration of solute. 1.2 Diffusion is important to living cells because it’s the way they take in materials from the environment‚ and they also prevent themselves
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DIFFUSION AND OSMOSIS: WHAT’S TO EXPECT? Diffusion and osmosis are very important in biological process. Diffusion is the movement of molecules or ions from a region of their high concentration to a region of their low concentration. Osmosis is the diffusion of water molecules across a semipermeable membrane. To gain a better understanding of diffusion and osmosis‚ we examine multiple experiments to show diffusion and osmosis in animal cell‚ plant cell and synthetic cell. In our first experiment
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