Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
Premium Cell culture Cell
Pre-Laboratory Questions 1. Yeast cells look like separate little spheres; they cannot walk or swim. Brainstorm methods by which yeast cells might communicate with each other. Record your list of possibilities in the space below. I. Signal Cascade II. Hormones III. Pheromones IV. Receptors embedded in the cell V. Growth near another cell Guided Activity: Table Charts Alpha – Type Culture Single Haploid Cells Budding Haploid cells Time 0 Field 1 3 3 Field 2 1 1 Field 3 8 2
Premium Yeast
Professor Dolinski English 112 2 April 2014 42 The movie 42 is a fairly new non-fiction movie describing the life history of Jackie Robinson‚ the first African American to play in the Major Baseball League (MLB) in the modern era‚ and his history-making signing with the Brooklyn Dodgers. 42 shows how hard it was in the 1940s to be a black man or woman‚ but Jackie Robinson and his team executive‚ Branch Rickey‚ did the unthinkable. The film 42 takes its audience on a journey through Jackie Robinson’s
Premium Jackie Robinson Major League Baseball
investigation I will be measuring the effects of temperature on the membrane permeability of beetroot. I will be measuring the amount of anthocyanin that will diffuse out of the beetroot. The way in which I will measure the anthocyanin is to check the light absorbency of the solution using a colorimeter. The higher the reading on colorimeter the more anthocyanin present in the solution To find out the permeability of the beetroot membrane I will firstly cut out cylinders of beetroot using a cork borer
Premium Laboratory glassware Energy Molecular diffusion
The aim of this lab session is to demonstrate the effect of different conditions on membrane permeability. 4.2 Learning Objectives In this Lab Session‚ students will acquire the skills to: - analyze the effect of different temperatures on membrane integrity. - analyze the effect of the organic solvent acetone on membrane integrity. 4.3 Introduction Even though membranes separate cells from their surrounding environment and different organelles within the cells from the cytoplasm
Premium Lipid bilayer Cell membrane Cell
(cont.) has on Membrane permeability of Beetroot cells. Hypothesis: I predict that as detergent concentration increases‚ the solution will become less clear‚ plus mass increases. The increases in mass will indicate that the water potential of the Beetroot cell is lower than that of the surrounding sucrose solution. The Beetroot discs will become flaccid and decrease in mass if the water potential of the surrounding solution is lower than the water potential inside the beetroot cell. I predict that
Premium Cell membrane Cell Protein
Hand Washing Experiment Outline I. Introduction a. This lab was conducted in order to understand and apply the scientific method. The experiment tested the effectiveness of reducing microorganism (bacteria) with the use of various hand soaps. b. Hypothesis: If the Harris Teeter Foaming Hand Soap is more effective in reducing bacterial growth than the Soft Soap hand soap‚ then there will be less bacterial/microorganism growth present on the agar plate for the Harris Teeter Foaming
Premium Soap Soap dispenser Scientific method
the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel onto the wet membrane and another Whatman paper was placed on top of the. The cathode plate was placed and started the transfer. Then the membrane was placed in TBST containing 5% milk for 60 minute while shaking. The membrane was rinsed
Premium Western blot Molecular biology Cell division
There are many ways in which substances can move across the cell membrane and in which then helps in the function of the body (cells‚ tissues and organs) overall. There are 3 processes that a substance can move across the membrane‚ theses are diffusion (simple and facilitated)‚ osmosis and active transport. Both diffusion and osmosis are passive processes‚ which mean they do not need energy to occur‚ whereas active transport does require energy. All three processes are involved in many functions
Premium
PDP Physics Lab Report – 01 NAME: Wang Xueqian DATE: 2014/06/21 Introduction In this experiment‚ we will investigate the relationship between the linear speed of an object and magnitude of centripetal force acting on it. We will use a stopper moving in a circle to create an upward force on the hanging mass; at equilibrium‚ the upward force on the mass will exactly equal the centripetal force. Apparatus Two-hole Stopper Plastic Tube Plastic Clip Electronic balance Hooked Masses Stop Watch String
Premium Measurement Force Mass