Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Mammalian Cell Culture Lab Manual Student guide of Cell Biology Laboratory Techniques 2012 Jay M. Feldman Cell Biology Professor Lagier 11/26/2012 Table of Contents I. Introduction * About the author page 2 * Preface page 3 II. Laboratory Techniques * Cell Culture Technique page 4-5 * Sterile Technique
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Lab 8 Cellular Respiration and Fermentation Objectives: 1. Observe the effects of cellular respiration on temperature in a closed system. 2. Investigate carbon dioxide production in both germinating pea seeds and crickets. 3. Perform an investigative study of the rate of cellular respiration in both pea seeds and crickets at various temperatures. 4. Compare the alcoholic fermentation of glucose‚ sucrose‚ and starch by yeast. Introduction All organisms must have a continual
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Title: The effect on temperature on beet cell membranes. Introduction A cell membrane or plasma membrane is very important in protecting cells. A cell membrane is a thin layer that protects the cells. The membrane is filled with lipids and proteins. The cell membrane protects the cell from ions and molecules that enter and exit the cells. I hypothesize that at -5 degrees Celsius‚ the color intensity will be a 1. I hypothesize that at 5 degrees Celsius‚ the color intensity will be a 2
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Methods for studying cells in the lab Centrifugation A centrifuge is a device for separating particles from a solution according to their size‚ shape‚ density‚ viscosity of the medium and rotor speed. Centrifugation can be used to separate cells from one culture or to isolate an organelle from the rest of the cell. The process relies on speed; the faster and longer the particle is centrifuged‚ the smaller the particles are that will be separated. Separation occurs by causing the larger particles
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treatment of a pH shock and neutralization‚ by depicting a constant percentage of immobile cells. Prior to the lab series‚ it was expected that the positive control would present a decreasing percentage of immobile cells‚ and the negative control would present an increasing percentage of immobile cells. The experimental groups‚ testing the hypothesis‚ was inferred to show a constant number of immobile cells‚ after the 60-minute light treatment. Colchicine is a substance that reduces inflammation
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John Leone Lab Partner(s) Ethan Davis & Ryan Hanna Date: 12/10/14 Instructor: Dr. Chuckran Period: H Plants and Animal Cells Abstract: In this lab‚ you will find out how plant and animal cells are alike and how they are different. The onion elodea cells were both plants‚ so they had a cell wall. The cheek cells were animal cells‚ so they did not have a cell wall. Eukaryotic cells contain structures called organelles that carry out life processes. Eukaryotic cells can be classified by the types of organelles they contain
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and centrifuged for 20 seconds. A polyacrylamide gel was assembled and the samples were loaded into it. The gel ran for approximately 45 minutes at 160 V. A western blot analysis was done in order to examine the Erk1/2 phosphorylation of the NIH3T3 cells. Three pieces of Whatman paper were wet in transfer buffer and placed on the anode plate. A wet PVDF membrane was placed on top of the Whatman paper. The gel was removed from holder and the stacking gel was removed. Forceps were used to place the gel
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Lab design –Voltaic Cells Aim Of Experiment In this experiment‚ we are going to make a battery by making an electron current between the metals Zinc and Copper. We aim two study the voltaic cells and the transfer of electrons from the metal to the ion. The tendency for the electrons to flow from one chemical to another‚ such as from the zinc metal to the copper ion as shown here‚ is something that can be channeled and controlled. Channeling the flow of electrons is what we will take up in this
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Electrochemical Cells Lab Report AP Chemistry Block 1 Analysis: The purpose of Part 1 of this laboratory is to construct a table listing the reduction potentials of a series of metal ions in order of ease of reduction. The series of half-cells is constructed by placing a piece of metal into a 1.0 M solution of its ions for each metal in the series. The metals are Cu‚ Fe‚ Pb‚ Mg‚ Ag‚ and Zn. The half-cells are connected by a salt bridge constructed of a strip of filter paper soaked in a solution
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The Effect of Temperature on Beet Cell Membranes and Concentration of Betacyanin Released Introduction Membranes are an important feature of plant cells and they act as a barrier that separates the interior of the cell from the external environment (Campbell et al.‚ 2008). Each membrane layer is composed of a phospholipid layer‚ which is semi-permeable and possesses the ability to control the movement of diffusion. Within the centre of a cell‚ the vacuole that is responsible for storage and
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