The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Cellular Biology Lab – Homework #3 Due to the week of Nov. 10-14th You may use the lab manual‚ pre-lab lectures‚ and credible internet resources‚ however you may not use your cell bio lab classmates as a resource. You will most likely see this material again on the Final and I highly encourage you to work individually and seek help from myself or your TA.Plagiarism will result in an automatic zero. 1. [15pt]In the cell bio lab‚ we use company manufactured gels‚ however you can make you own polyacrylamide
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Investigating the Effect of Light Wavelength on the Rate of Photosynthesis Method Take a 250cm3 beaker and fill it with distilled water. Use distilled water so there are no impurities to disrupt photosynthesis. Then take x cm’s of elodia and put it in the beaker. Cover the elodia with the large end of a funnel and the tip of the funnel cover with a test tube. Then direct a light onto this experiment. Measure the amount of bubbles that come from the elodia for x minutes. A prediction
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Cellular Transport Worksheet KEY OSMOSIS Copy the pictures below‚ and write the correct type of solution underneath (isotonic‚ hypertonic‚ or hypotonic) Hypotonic Hypertonic Isotonic Hypertonic means there is a GREATER concentration of solute molecules OUTSIDE the cell than inside. Hypo tonic means there is a LOWER concentration of solute molecules OUTSIDE the cell than inside. Isotonic means there
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Shier‚ Butler‚ and Lewis: Hole’s Human Anatomy and Physiology‚ 10th ed. Chapter 4: Cellular Metabolism Chapter 4: Cellular Metabolism I. Metabolic Processes A. Introduction 1. Metabolism is the sum total of chemical reactions within cells. 2. In metabolic reactions‚ the product of one reaction serves as starting materials for another metabolic reaction. 3. This chapter explores how metabolic pathways supply a cell with energy and how other biochemical
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ids Acids‚ Bases‚ Buffers and Respiration Darlene E. McDonald WCCC Fall 2012 Ap Lab 071-07 Dr. Roxanne Levandosky August 31‚ 2012 I. INTRODUCTION: A.PURPOSE: a. To define and understand the key terms. ~acid ~buffer system ~base ~acidosis ~pH ~alkalosis ~pH scale ~hypoventilation ~pH indicators ~hyperventilation b. To determine the acidic or base nature and actual pH of various substances. c. To discuss the formation of the carbonic acid/bicarbonate ion
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Based off the results from this lab the student was able to determine and have an overall better understanding of the variables that affect the rate of photosynthesis in a plant. The first variable the student tested in the lab was 30cm of light with CO2 present. The student started this trial with all 10-leaf disks and timed how many leaf disks came up every minute for 15 minutes. One minute into the trial and the student recorded that no leaf disks had yet to rise to the top of the solution. At
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Plan I plan to investigate how light intensity affects the rate of photosynthesis in pondweed. The rate of photosynthesis will be measured by the number of bubbles given off (considering that oxygen is produced as a waste gas during photosynthesis). The light intensity is measured by the distance between the lamp and pondweed. Fair test: Fix: the amount of pondweed‚ the amount of water and sodium hydrogen carbonate solution in the test tube‚ the time allowed for each experiment Change: the
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Highlights of the Marketing Strategies adopted by Idea Cellular:. (I) AB as the Brand Ambassador-Though the company was a bit late in appointing Abhishek Bacchan as the brand ambassador of the company(In October 2007). The company’s focus on its ads also shifted from highlighting it’s tariff plans and network coverage to use of mobile telephony to resolve social issues. It also tied up with the IPL‚ Mumbai Indians‚ and players like Sachin Tendulkar and Zaheer Khan promoted the brand. (II) Focus
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If i increase the intensity of the rate of photosynthesis would drastically increase which is the light bubbles would be emitted in the jar in one minute because of the fact that the more photons of light that fall on a leaf then more chlorophyll molecules would be ionised and the more ATP and NADPH are generated If i increase the intensity of the rate of photosynthesis would drastically increase which is the light bubbles would be emitted in the jar in one minute because of the fact that the more
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