Chapter 3 Centrifugation Biochemistry and Molecular Biology (BMB) 3.1 Introduction 3.2 Basic Principle of sedimentation 3.3 Types‚ care and safety of centrifuges 3.4 Preparative centrifugation 3.5 Analytical centrifugation Analytical Biochemistry (AB) 3.4.3 Ultracentrifugation Koolman‚ Color Atlas of Biochemistry‚ 2nd edition 1 General Steps in Biochemical Separation 2 Separation of Macromolecules Chromatography‚ precipitation Electrophoresis‚ ultracentrifugation 3
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Ondiano‚ Eliza L. MSEST – 1 CENTRIFUGATION Centrifugation is a process which through rapid spinning imposes high sustained centrifugal force on suspended particles‚ or even molecules in solution‚ and causes separations of such matter on the basis of differences in densities. More-dense components of the mixture migrate away from the axis of the centrifuge‚ while less-dense components of the mixture migrate towards the axis. Effectively‚ the centrifuge substitutes a similar‚ stronger force
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DEPARTMENT:Biochemistry LEVEL:200L SCHOOL:university of ilorin ASSIGNMENT: DIFFERENCES BETWEEN DENSITY GRADIENT CENTRIFUGATION AND DIFFERENTIAL CENTRIFUGATION‚AUTORADIOGRAPHY AND CYTOCHEMISTRY. Density gradient centrifugation Separation of molecules and particles on the basis of buoyant density‚ by centrifugation in a concentrated sucrose or caesium chloride solution. Differential centrifugation This is a technique that allows the separation of cells‚ organelles and macromolecules‚ depending
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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Lab Report 3 In this lab‚ we will take a trip to the planetarium lab and will learn about Right ascension and declination‚ and altitude and zenith. After looking at the different points shown‚ we will log the altitude and zenith in the chart in our lab manual. Now we will look at the same points and label the right ascension and declination. Then we will learn about the easiest way to locate the star Polaris. As we started the first program‚ we answered a few questions to make sure we knew
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This lab report will be detailing the steps taken and the results discovered when using spectrophotometry to determine the percentage of copper in a copper-clad penny and the thicknes of the copper layer on the copper-clad penny. After 1982‚ copper coating has been used in the creation of the penny because the cost of pure copper has increase to the point that the amount needed t omake a penny cost far more than the actual value of the penny. This lab allowed us to see just how much copper coating
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