EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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American Chemical Society databases were investigated through Scifinder Scholar Version X to find any information about Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate. There was no previous information present. Then‚ the microscale synthesis and characterization of Butyl 2-Cyano-(3‚5 dichloro-phenyl)-2-propenoate was conducted with the base catalyzed Knoevengael condensation of the butyl cyanoacetate and corresponding aldehyde (gkharas/235_14F/235_14F_Lab_2.pdf) and analysis. The elemental analysis that
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Allegory can be defined as a story that can be interpreted to reveal a moral‚ which is usually a generalization about human existence. Both short stories‚ “The Chaser” by John Collier‚ and “Dr. Heidegger’s Experiment” by Nathaniel Hawthorne are both examples of allegory. Using the themes of change‚ self indulgence and consequence‚ the authors are able to successfully tell their allegory in a way as to lure in the reader. Hawthorne and Collier both use symbolism and metaphor throughout their stories
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Nathaniel Hawthorne was born in Salem‚ Massachusetts in 1804. After his graduation from Bowdoin College in Maine‚ he quickly became a well-known author of literary tales concerning early American life. Between 1825 and 1850‚ he developed his talent by writing short fiction‚ and he gained international fame for his fictional novel The Scarlet Letter in 1850 (Clendenning 118). Rufus Wilmot Griswold stated‚<br><br>The frivolous costume and brisk action of the story of fashionable life are easily depicted
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John Brown as a "Martyr” A martyr is defined as a person who is put to death or endures great suffering on behalf of any belief‚ principle‚ or cause. To many people back in eighteen fifty-nine‚ this defined a man named John Brown in many ways. John Brown was a devoted abolitionist who had been important in the conflict of slavery in Kansas. In October of eighteen fifty-nine‚ Brown led an interracial group of men who took over a federal arsenal in Harper’s Ferry‚ Virginia‚ hoping to spark a slave
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Comprehending the Mind’s Aging Eye "The Allegory of the Cave‚" by Plato‚ explains that people experience emotional and intellectual revelations throughout different stages in their lives. This excerpt‚ from his dialogue The Republic‚ is a conversation between a philosopher and his pupil. The argument made by this philosopher has been interpreted thousands of times across the world. My own interpretation of this allegory is simple enough as Plato expresses his thoughts as separate stages.
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of The Lion‚ the Witch‚ and the Wardrobe wrote this is novel in 1949. The novel is basically about religion faith and Christianity. And testing your faith kind of. An allegory is like a story that is compared to the one you’re looking at. Something about it and that tells a story behind that you may not see or realize. The allegory in this novel is that Aslan is like Jesus Christ and in the story it was kind of like a test in the children’s faith. Seeing how they would believe and think. My claim
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In " A Goodman Is Hard To Find" Flannery O’Connor suggest that the Misfit is psychologically unstable based off his murderous actions as well as his blatant denial of his actions. O’ Connor suggest that the Misfit suffers from anxiety and uses denial as a defense mechanism by mentally distorting his actions as more acceptable. The Misfit has escaped from jail‚ and was put in jail because he allegedly killed is father. His denial is evident because he claims that this is untrue : "It was a head doctor
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Williams’ characterization of Blanche as a character of many layers and different emotions is particularly evident in this scene as he presents her in different lights through different mediums. In this scene‚ Blanche is presented as afraid. This effect is achieved through Blanche’s actions‚ which are revealed to us by stage directions. “She looks fearfully after him” this explicitly unveils to the audience Blanche’s reaction to Mitch’s arrival as well as his attitude. The adverb “fearfully” adequately
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Allegory in Lord of the Flies In William Golding’s Lord of the Flies‚ which is set during World War II‚ English school boys‚ escaping war in England‚ crash on a deserted tropical island. From the protected environment of boarding school‚ the boys are suddenly thrust into a situation where they must fend for themselves. In order to survive‚ the boys copy their country’s rule for a civilized life by electing a leader‚ Ralph. He promises order‚ discipline‚ and rules for the boys so that they form
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