Abstract The experiment‚ entitled Extraction and Characterization of Proteins‚ aims to isolate casein from milk and albumin from egg; to explain the methods employed for protein extraction; to apply spectrophotometric methods in characterizing and quantifying extracted casein and albumin. The experiment was divided into 2 parts; the extraction of Albumin from egg and the determination of protein concentration via the Warburg-Christian method and Bradford Assay method. In the first part‚ egg
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Crystal Lai Mr. Peake English 9 E November 27‚ 2007 Of Mice and Men by John Steinbeck: Characterization of George George is calm and collected‚ despite his solemn mood after Curley¡¯s wife is discovered dead. In that moment‚ the ill-fated protagonist begins to understand what must be done and prepares himself for the final stretch‚ and yet his hand is trembling as he points the barrel of the gun to the back of Lennie¡¯s head. Logical‚ clever but fiercely loyal and compassionate‚ George Milton
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”Characterization of the main characters” “Indian Camp” Nick followed his father to an Indian camp to witness his father help a women through childbirth. At first glance “Indian Camp” seems to be about a boy’s right of passage experience‚ as he witnesses a child’s birth. This beautiful feminine act is however described through masculine eyes‚ and therefore is more about the father’s development than it is about Nick’s or the Indian woman. But this story also unarguably represents an initiation‚ or
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Colour recession & Negative space “The Sower” Vincent Van Gogh‚ painted in 1888. He uses a silhouette to represent the raising of ordinary people and the sun creating a halo over his head. He also uses colour recession because there are colour opposites present. Positive space is the tree and the sower‚ the background is the negative space. “Sunflowers” Vincent Van Gogh painted in 1888. It’s not necessarily big on positive and negative space‚ but it is great for colour opposites and recession
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EXPERIMENT NO. 15 PROTEIN CHARACTERIZATION BY ELECTROPHORESIS Abstract The molecular weights of protein extracts were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Two sets of four protein samples‚ standard bovine serum albumin (BSA)‚ invertase‚ egg albumin‚ and casein‚ were prepared; one set containing β-mercaptoethanol (BME) while the other did not. These were then analyzed through SDS-PAGE with 12.5% resolving gel‚ prepared using 2 M Tris-HCl at pH 8
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Protein Hydrolysis and Characterization Group 7 Niez‚ Robert Francis‚ *Orbin‚ Alfonso Ricardo* Parro‚ Athena Emmanuelle Peralta‚ Christian Department of Biological Sciences‚ University of Santo Tomas‚ Manila‚ Philippines • Abstract Hydrolyzed Protein is protein that has been hydrolyzed or broken down into its component amino acids. While there are many means of achieving this‚ two of the most common are prolonged boiling in a strong acid (acid-HVP) or strong base or using an enzyme such
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Freeman was born in a time when women were married at a young age. In the story Lousia was considered an old maid. She waited fourteen years for one man. Lousia was costumed to doing her own routine. She would clean‚ garden and take care of her old yellow dog named Caesar. When Freeman describes the dog in her writing it feel that she is also talking about Lousia. Both Lousia and Caesar were chained and locked away in different ways. Lousia had the freedom to leave if she chose to but did not. Lousia
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Vincent Van Gogh‚ genius or madman? ‘The connection between genius and mental illness is paradoxically both clearer and murkier than that between genius and high intelligence.’ (Published on March 31‚ 2011‚ Andrew Robinson) Van Gogh‚ possibly the most famous example of a known genius who beared the title of a mentally ill individual‚ had severe depression‚ inflicted serious injury on his own earlobe in the year of 1888‚ admitted himself to a mental facility‚ and shot himself in 1890‚ yet he is one
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Isolation and Characterization of Complex Lipids Shea Kathleen Guinto‚ Peter Julius Dale Inocando*‚ SooHyun Jung‚ Jurilla Camille Louise Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract In this experiment an egg yolk will serve as the sample to isolate lipids and separate them into two classes which is the phosphorylated lipid and the non-phosphorylated lipid. The isolated lipids are then characterized through a series of color chemical tests along with the given
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Vincent Van Gogh’s strength as an artist comes from both the relationships with the people that he is close with and the connection that he has to the common man as shown in his many paintings of friends‚ family‚ and strangers‚ and the techniques that he has incorporated into them‚ learned from his many artist acquaintances. When Van Gogh focused on average workers in his early career‚ his drawings represented a side of humanity that no other painter was quite able to achieve. He was born on March
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