The Effect of substrate on the Rate of Respiration on Yeast1 Justine Maturan Group 4 Sec. Y – 5L November 18‚ 2014 ________________________________________________________________ 1A scientific paper submitted in partial fulfilment of the requirements in General Biology I Laboratory under Prof. Susan Sedano‚ 1st semester 2014-2015 ABSTRACT In order to determine the effect of the substrate on the rate of respiration of yeast‚ Durham test tube method was used in the first experiment
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was to find the relationship between temperature and the enzyme activity of amylase. This was achieved by attaining amylase enzyme‚ starch solution and potassium iodide (determines if enzymes hydrolyses the starch solution)‚ water bath and a hot plate. The temperatures used for this experiment were room temperature‚ 37oC‚ 60oC‚ 80oC‚ and 90oC. The hypothesis developed was that as the temperature increased‚ so will enzyme activity. Therefore‚ the ability of the enzyme to break down the starch
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Effect of temperature on enzyme activity In this experiment… Independent variable: Temperature of the amylase Dependent variable: Enzyme activity which is measured by the time for disappearance of starch Controlled variables: Volume of amylase; volume of starch solution; concentration of amylase; concentration of starch solution Prediction of results i) At low temperature‚ the rate of amylase activity is very low. ii) At optimum (=best) temperature‚ the rate of amylase activity is the
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Rate of Reaction that Enzyme Concentration‚ pH‚ and Temperatures Have on the Amylase Enzymes Color Disappearance Abstract: Compare reaction rates of the concentrations‚ pH’s‚ and temperatures of the enzyme Amylase. At what concentrates do the substrate molecules collide with each other‚ making the reaction possible? At what pH levels do the 3D molecular structures change breaking the H-bond and/or denaturize? At what temperatures do the collisions of the substrate molecules happen? Proteins
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This study focuses on the effect that temperature has on the enzyme amylase. Enzymes are composed of proteins and acts as a catalyst to speed up the rate of a specific chemical reaction by lowering the activation energy. Reactions do not need enzymes to occur‚ but the human body and other living organisms depend on the use of enzymes in order for biochemical reactions to happen in milliseconds. Each enzyme contains a distinct three dimensional structure which is related to its particular function
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EFFECT OF TEMPERATURE‚PH AND SUBSTRATE CONCENTRATION ON ENZYME ACTIVITY | Aim To investigate the effect of Temperature‚Ph and substrate concentration on the rate of enzyme activity. Hypothesis
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main environmental factors that can affect the enzyme’s activity are temperature and pH‚ and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and
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and not starch‚ is because saliva contains an enzyme known as salivary amylase which catalyses the breakdown of starch to produce sugars such as maltose (Sherwood‚ 2013). When testing the effect of the salivary amylase concentration‚ we observed that the solution containing the most saliva (3ml)‚ become colourless faster than the solution which contained the least saliva (1ml). This proves that an increase in enzyme concentration (increase in saliva)‚ will cause an increase in the reaction rate (Bennett
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1. PIE CHART This pie chart shows Mark’s monthly budget. The highest designation of his budget will go to his foods with 45% of his total allowance. Next is for lodging with 30% followed by the projects and fare which will have 10%. The least designation for his budget will be for his savings which has 5% only. 2. BAR GRAPH The bar graph shows the yearly tourist count for the provinces of region V. the province of Albay got the highest number of tourist with 450 000. It is followed by the provinces
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particular enzyme‚ in this case amylase. Hypothesis: In this investigation I expect as the pH reaches the optimum level‚ the rate of reaction will be fastest‚ compared to other pH levels. It is also suspected that after the enzyme has reached optimum level the enzyme activity will decrease. Through further study of the optimum level of amylase I found that the enzyme usually has an optima pH of 8. It is known that the pH of an enzymes environment will affect its activity rate. Discussion: In
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