experience in bacterial morphologies in prepared wet-mounted slides and interpreting the findings of bacteria through direct and indirect staining technique. Hypothesis – The experiment will allow for further insight into stained organisms‚ allowing extended contrast‚ differentiating shape and structure through utilization of microscope. Procedure – Instructions followed as per procedure‚ specimens viewed through v-scope‚ using 10x‚ 20x‚ and 40x stained wet-mount. Observations compared amongst one another
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experiment is to differentiate between prokaryotic and eukaryotic cells. Also to distinguish among plant‚ animal‚ and protest cells and identifying the organelles that are evident in them. Materials and Methods A wet mount with a sample of Elodea (a common pond weed) was prepared. The wet mount is prepared by placing a drop of water on a glass slide. A very small piece of the Elodea is placed in the water and covered with a slip cover. The slide was then placed under the light microscope and observed
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Bacterial Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms : Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. It was hard to tell what I was looking at. There were a number of amoeba shaped cells of varying sizes. There were five darker areas circular in nature. There were also three very large‚ odd shaped areas with
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Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation There was several amoeba shaped cells that varied in size. There were five somewhat darker areas that were circular in shape. There were also three large‚ oddly shaped areas that had very distinct edges. Part 4: Direct Staining:
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Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained specimens and record your results. Questions: A. What are the advantages
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Shapes Familiarize yourself with each morphological type to use as a comparative tool for the remainder of the activity. Record your observations. Part 2: Disinfecting Your Area to Use Live Organisms: Part 3: Viewing Live Organisms – Wet Mount Preparation Record your observations. Part 4: Direct Staining: Record your observations for each sample. Part 5: Indirect Staining: Examine the stained specimens and record your results. Questions: A. What are the advantages
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2. Make a wet mount of your cheek cells (see p. 4-6 for instructions). Examine your slide under the compound microscope and draw a few cheek cells at 400X in the space provided. Be prepared to discuss and present your drawings Make an Elodea leaf wet mount: obtain an Elodea leaf and make a wet mount — do not use methylene blue or other dye in this case. Examine the wet mount with your compound microscope at 400X. Focus on the fleshy layer inside
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characteristics). In this lab experiment‚ bacterial morphology was examined by observing both stained and unstained organisms. A wet mount is a preparation process where a live specimen in culture fluid is placed on a slide and the organism is free to move about. In the wet mount slides provided via LabPaq software with cheek‚ dental plaque‚ and yeast specimens were observed. The wet mount preparations were difficult to observe because of poor contrast‚ however‚ a common occurrence in the specimens were cells
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Title: To examine an animal and cheek cell using a compound microscope Date: 25th September 2012 Lab Partner: xxxxxxxxxxxxx Objective: The main aim of this experiment was to prepare a wet mount of human cheek cells and a wet mount of plant cells and examine them both under a compound microscope. Introduction: Cells are the basic units of life which make up every structure in the human body. They are the smallest units which are living. All cells contain different organelles which
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Onion 10. Cheek cell 11. Prokaryote cell suspension Methodology Part 1 : Onion cell wet mount 1. A translucent piece of tissue from onion was peeled. 2. The piece of onion was placed on a glass slide and a drop or two of the Methylene blue solution was added. 3. The onion cell was observed under low‚ medium and high power. A drawing of one cell was made‚ using a pencil and all of its part are labelled. Part 2 : Cheek cell wet mount 1. The inside lining of your cheek with a toothpick
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