CHEM 2303 ANALYTICAL CHEMISTRY II LECTURE 2 – APPLICATIONS OF SPECTROPHOTOMETRY TENTATIVE LECTURE SCHEDULE Date Topics • Presentation / discussion of syllabus • Tips for success • Laboratory • Introduction to the analytical process • Sample preparation Jan 9th‚ 2015 • Spectrophotometry • Properties of light • Absorption of light • Excited states • Luminescence • Applications of spectrophotometry • UV-Vis spectroscopy • Fluorimetry Jan 16th‚ 2015 • Fluorescent staining‚ green fluorescent protein
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LAB REPORT NUMBER TWO DATE: 3/25/2010 inal attachment Lab Experiment number 11 PURPOSE: To learn the Gram stain technique‚ the reason for the stain‚ and how to identify the results of the organisms stained. MATERIALS: Bunsen burner‚ inoculating loop‚ staining tray‚ glass slides‚ bibulous paper‚ lens paper‚ oil‚ and microscope METHODS: Apply Crystal Violet (Primary stain) for 1 minute. Rinse with D-water Apply Iodine (Mordant) for 1 minute. Rinse with D-water. Apply Alcohol (Decolorize) for
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Scientific Writing and Standard Components of a Lab Report INTRODUCTION Writing is a powerful tool. Effective communication is necessary to succeed in any field. As a professional‚ you must communicate with both your colleagues‚ and the community at large. Writing is the means of showing the value of your work. The specific style of writing in science is based on the unique audience of readers. In addition‚ writing allows one to organize one’s thoughts and clarify ideas‚ and it fosters abstract
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CHEMISTRY 17 1ST LONG EXAM REVIEWER I. ELECTRONIC STRUCTURE OF ATOMS -arrangement of atoms’ electrons -the number of electrons in an atom -distribution of electrons around the nucleus and their energies • ATOMS *Billiard Ball Model (JOHN DALTON) *Plum Pudding Model (JOSEPH J. THOMPSON) *RUTHERFORD’s Model of Atom -atoms are tiny‚ hard‚ indivisible spheres -electrons (negative charge) occupy the atom - Atom is mostly empty space with a dense nucleus • PROBLEMS with Rutherford’s Model
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EXPERIMENT 1: Proteins -polypeptides compose of > 50 amino acids Functions 1. Enzymes or subunits of enzymes-enhancing the rates of reactions 2. Structural or Mechanical roles 3. Immune response roles 4. Storage and transport of substances 5. Source of amino acids for organisms that cannot synthesize amino acids naturally ISOLATION- disruption of cell membranes to release cell contents; separation for other contaminants 1. CENTRIFUGATION 2. SALTING OUT-water
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Lab 1.1: Reading Binary I. 2 2 2 2 2 2 2 2. You use the method of subtraction‚ if you can subtract the number that corresponds with the value you “2” you are dealing with you need to place a 1 under the 2 that corresponds with the number you are subtracting. This is like saying yes the number works. If it doesn’t subtract you place a zero underneath the “2” that you are dealing with‚ this is like saying “no” it doesn’t represent anything. That is the basic formula for doing binary
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Period 12 Chemical Aspects of Life & Spit Lab ABSTRACT: The objective for the Spit lab was to test two different types of crackers for the presence or absence of starch and of reducing sugars. Also‚ to test the chewed cracker‚ the one that didn’t have a reducing sugar‚ for the presence or absence of a reducing sugar with the saliva in it. Adding on‚ another part of the objective is to determine the effect of amylase on starch. For the Chemical Aspects lab‚ the objectives were to test for the presence
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Formula of a Hydrate Lab Design: Problem: What is the chemical formula for a hydrated copper (II) sulphate compound? Controlled Variable: The mass of hydrated copper (II) sulphate. Responding Variable: The mass of dehydrated copper (II) sulphate‚ mass of H2O Materials: Ones on the handout + Hot plate. Procedure: 1. Mass 3.00g of hydrated copper (II) sulphate using electronic balance 2. Measure the mass of a thin‚ crucible dish using electronic balance 3. Gently pour hydrated
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Solvent/Water Trap Diagram Rodney Atwaters Halle Bair May 28‚ 2013 Chemistry 36100 Introduction The purpose of this lab was to produce benzoic acid by mixing sodium benzoate with hydrochloric acid. The Hydrochloric acid used in the experiment was a stock solution made by mixing HCl Acid and deionized water. HCl acid‚ historically called‚ muriatic acid or spirits of salt‚ hydrochloric acid is produced from sulfuric acid and common salt‚ NaCl (Princeton.edu). Since the early 1900 ’s‚ sodium
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Back Laboratory Program SAFETY IN THE CHEMISTRY LABORATORY 786 Pre-Lab Extraction and Filtration 790 1-1 Mixture Separation 792 1-2 Water Purification 794 3-1 Conservation of Mass 798 4-1 Flame Tests 801 Pre-Lab Gravimetric Analysis 828 13-1 Separation of Pen Inks by Paper Chromatography 830 13-2 Colorimetry and Molarity 834 14-1 Testing Water 838 804 7-1 Separation of Salts by Fractional Crystallization 806 7-2 Naming Ionic
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