process heating a liquid to form vapor which is then collected when cooled and separated from the original liquid. The purpose of this experiment was to determine the identity of an unknown substance using simple and fractional distillation. Gas chromatography analysis the components of a mixture by separation of the materials by their different boiling points. EXPERIMENTAL The simple distillation apparatus was assembled using a 10-mL round bottom flask‚ distillation head‚ thermometer‚ adapter‚ thermometer
Free Distillation
Intermolecular forces – design lab Research question: To calculate the solubility of equal masses nitrates of four di-positive ions (Lead Nitrate‚ Barium Nitrate‚ Copper Nitrate & Ferrous Nitrate of 0.5 M concentration) in an equal volume of water and relate this to the ionic radius of the element and the molar mass of the nitrate chosen and hence comment on the intermolecular forces in each of these nitrates. + write mass Justification of the research question: I was particularly interested
Premium Solubility Mass Ion
In order to purify CelB2 protein bound to maltose-binding-protein‚ amylose affinity chromatography was performed. The amylose resin present in 20% ethanol was first diluted by adding 10 mL of 20 mM TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA and centrifuged at 700 rpm for 5 minutes. After decanting the buffer‚ another 10 mL of TrisHCl‚ pH 7.4‚ 0.2 M NaCl‚ 1 mM EDTA was added to this resin solution and centrifuged at 700 rpm for another 5 minutes to further dilute the ethanol concentration in the resin
Premium Ethanol Enzyme Chromatography
Chemistry 1211K Lab Report Briana Limage Drawer #D20 Tuesday December 2‚ 2014 Lab Day and Time: Tuesday 2-5 Unknown # 4224034-DF14 Introduction The purpose of this semester long experiment was to determine an unknown organic acid. An organic acid is an organic compound with acidic properties. A base reacts with acids to form salts. Titrations are used to determine the concentration of unknown substances. The purpose of the KHP experiment was to determine the molarity of NaOH. HCl
Premium PH Sodium hydroxide Acid
Lab 4: Photosynthesis and Chromatography of Spinach Leaves Discussion: The chromatography of the spinach leaves was taken to establish what pigments were present in the chloroplasts of the leaves. The solvent carried and separated the different pigments up the cellulose paper. The solvent moved up the paper because of capillary action because of adhesion of the solvent molecules and the paper. The pigments dissolved in the solvent also were carried up the paper and were separated because
Free Photosynthesis
prelab assignment to be allowed to participate in the lab? Question options: A week before the lab Day before the lab at 10 pm The morning of lab day at 8 am One hour before the lab Question 2 1 / 1 point Which materials/chemicals belong in the desiccator? Question options: Drying agents such as anhydrous magnesium sulfate silicagel 1 M NaOH aqueous standard solution aqueous extract of your reaction alumina for chromatography Question 3 1 / 1 point Vacuum tubing is the thick-walled
Premium Acetic acid Carboxylic acid Ester
SEPARATION OF MIXTURE OBJECTIVE: To separate the components of a mixture based upon physical characteristics of each component within the mixture. Secondly‚ to determine the mass of each component by using the knowledge of separation techniques. SAFETY REQUIREMENTS: Do Not Pipette By Mouth Read the Chemical Safety Information Dress Appropriately Identify the Safety Equipment Don’t Taste or Sniff Chemicals Don’t Casually Dispose of Chemicals Down the Drain MATERIALS:
Premium Filter paper Mass Mixture
Title: Separation of Mixture of Solids - Experiment 3 Date: 9/21/13 Purpose: This experiment is designed to show the different ways to separate solid mixtures into individual elements. It will also determine whether a substance can be considered “pure” or not‚ as well as determining the solubility of each mixture in water. Procedure: 1) Weigh the amount of the mixture on the digital scale. Separate the Iron out by using a magnet to filter out the Iron element. 2) Take the remaining mixture
Premium Water Distillation Separation process
1. The use of microscale techniques does not change the hazardous nature of the reagents we use. Briefly describe the hazards associated with even small amounts of each of the following: a. concentrated hydrochloric acid‚ HCl HCL may be corrosive. It can cause burns to dosy tissue and can be deadly if inhaled or swallowed. b. sodium hydroxide‚ NaOH‚ solution NaOH is a very corrosive chemical and contact with it can cause burns to body tissue and
Premium Chemistry Hydrogen Sodium
Cydney Kessler Date of Experiment June 13‚ 2015 Report Submitted: June 15‚ 2015 Title: Laboratory Techniques and Measurements Purpose: In this experiment I will determine the mass‚ density‚ volume‚ lengths and temperatures of different objects and liquids to help me better understand the importance of Chemistry and how Chemistry plays a big role in our everyday lives. Procedure: In the experiment‚ I use devices such as a ruler‚ beakers‚ flasks‚ a scale and cylinders
Premium Volume Water Density