ENVIRONMENTAL TECHNOLOGY DEPARTMENT Lab Report ROOM NO: FE E309 EXPERIMENT NO : 8 TITLE : Thin Layer Chromatography of Food Dyes Submitted by Class Partners Instructor : Lyndsay Grover : BIOL 10000 lab. : Awatif Hagelamin : Farag Soliman Date lab performed : February 3‚ 2011 Date of submission : February 10‚ 2011 FENNELL CAMPUS HAMILTON‚ ONTARIO 1/3 Purpose: The purpose of this experiment was to observe the reactions of food dyes with thin layer chromatography paper in order to find the
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Abstract In this experiment paper chromatography was used in order to identify two unknown amino acids using eight known amino ones. The two unknown ones were identified by comparing the distance they travelled up the chromatography paper and their Rf values to the corresponding values of the other eight known amino acids. The unknown amino acids identified were Glycine and Methionine. Introduction Proteins in cells are important in many ways. There are different types of proteins such as
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Chemistry 2283g Experiment 1 – Alkyl Halides EXPERIMENT 1: Preparation and Reactivity of Alkyl Halides Relevant Sections in the text (Wade‚ 7 ed.) • 6.7 (p. 228) Reactions of alkyl halides • 6.8 – 6.12 (p. 229 – 242) The SN2 reaction: generality‚ factors affecting SN2 reactions‚ substrate reactivity‚ mechanism • 6.13 – 6.14 (p. 243 – 249) The SN1 reaction: mechanism‚ stereochemistry General Concepts The most common of the variety of methods available for preparing alkyl halides is the replacement
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the sample for gas chromatography. Introduction: Distillation has been used since antiquity to separate the components of mixtures. This method has been used to manufacture different organic chemicals but the most recent application is the refining of petroleum in order to produce fuels‚ lubricants and petrochemicals. In this experiments‚ the separation of 2 distilates were carried out using the differences in boiling points and the data was used for a gas chromatography test. Objectives:
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CHROMATOGRAPHY OF PLANT PIGMENTS Marquez‚ Ma. Rica Paulene‚ Moises‚ Patrisha Kate‚ Policarpio‚ Jairus Paolo‚ Rolda‚ Zylene Joy Department of Biology‚ College of Science‚ University of the Philippines Baguio April 23‚ 2013 ABSTRACT The objective of this experiment was to apply the technique of paper chromatography as a method for separating individual plant pigments contained in plant tissue extracts containing pigment blends. The process of chromatography separates molecules because of the
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The order of presentation is strongly recommended by the National Mathematics and Science Initiative (NMSI) UNIT 1 Foundations / Nomenclature / Stoichiometry (Summer Work and Introductory Review) these topics are all covered in College Prep and Honors Chem Chapter 1 Introduction: Matter and Measurement 1.1 The Study of Chemistry The Atomic and Molecular Perspective of Chemistry 1.2 Classifications of Matter States of Matter Pure Substances Elements Compounds Mixtures
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Ink Chromatography Lab Background Information Paper chromatography is an analytical method that is used for separating and identifying mixtures of substances into their smaller parts. Paper chromatography works because the ink used contains several dyes (pigments) that when place on porous paper are dissolved in a solvent by capillary action. When the pigments (solutes) are dissolved in the solvent (water & alcohol mixture) they move through the paper at different rates depending on their
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acid solution would be titrated by slowly adding drops of solution into NaOH‚ and the volume of base needed to react with the acid is measured throughout. By using those data‚ the students are able to find what they were trying to calculate. On this lab‚ the students found the unknown acid‚ 4.36 x 10-5‚ and the equivalence point of 8.4‚ as well as the value of pH. Purpose: The purpose of the experiment was to utilize the techniques of titrations to measure the concentration of an acid or base in
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unknown substance or determine a known substance’s level of purity. When organic substances are mixed together in varying degrees they take on a melting characteristic that is lower and broader than in its pure form. This property was manipulated in the lab to observe the various melting points of Naphthalene and Biphenyl when the percentage of composition was altered. A eutectic point of 45 °C at 52% Naphthalene was determined from the class data. An unknown crystalline substance was identified to be
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Unit 2 PPA 1 Complexometric Determination of Nickel using EDTA Introduction: The Aim of the experiment is to determine the percentage of nickel salt using a complexometric technique. Ethylenediaminetetraacetic acid (EDTA) is a hexadentate ligand which forms stable complexes with most metal ions‚ thus is widely used to determine metals in complexometric titrations. EDTA can be represented as H4Y and in alkaline conditions‚ it exists as Y+ ions: The Y4- ions form 1:1 complexes with metal ions
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