exchange Chromatography Ion exchange chromatography is a process for separating proteins and other molecules in a solution based on differences in net charge. Ion Exchange Chromatography relies on charge-charge interactions between the proteins in your sample and the charges immobilized on the resin of your choice. Ion exchange chromatography can be subdivided into cation exchange chromatography‚ in which positively charged ions bind to a negatively charged resin; and anion exchange chromatography‚ in
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When people hear candy chromatography‚ they don’t know what to think about it. They of course know that it had something to do with candy. But just don’t know what. Candy chromatography is fun and simple. Candy Chromatography is a fun project‚ plus it has to do with candy. Candy Chromatography is an experiment to find out the true colors of the dyes in candy. When you put a skittle or an M&M in your hand in hot weather‚ the candy tends to bleed the color. And sometimes it shows more than one color
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What is chromatography- Chromatography is the separation of mixture by passing it in solution or suspension or as vapor. It’s a technique for separating mixtures into the components this needs to happen in order to do the 4 things analyze‚identify‚purify‚quantify. Many scientist use this to do the 4 steps. When analyzing its used to examine the mixture and to find out the relation with one another. In purifying you need to seperate or take away and put it by itself for further study. In identifying
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www.igcse.at.ua ORGANIC CHEMISTRY OIL and its many useful PRODUCTS The origin of oil Crude oil is formed from organic material of the remains of plant and animal organisms that lived millions of years ago. These remains form sediments eg at the bottom of seas‚ and become buried under layers of sedimentary rock. They decay‚ without air (oxygen)‚ under the action of heat and pressure to form crude oil over millions of years. It is a fossil fuel because it is formed from
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Syallubus for CHEM 1301 General Chemistry I Fall 2011 SCIE 2.106‚ M‚W: 2:35-3:50 Instructor Name: Dr. J. G. Parsons Email: parsonsjg@utpa.edu Office: Science room number: 3.348 Phone: Office : 381-7462 Office Hours: M/W: 1:30-2:30 or by appointment CRN: 12626 REQUIRED MATERIALS: Text Book: Chemistry by Julia Burdge (ISBN 978-0-07-302554-4) Scientific calculator (ARIS software package Required) RECOMMENDED MATERIALS: Burdge study guide Blackboard web course and a UTPA email address. CREDIT: Lecture
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Objective: To determine the concentration and equilibrium constant for a reaction of colored product using absorption spectroscopy‚ to create a calibration curve for determining equilibrium concentrations by applying Beer’s law and to force the completion of a reaction by applying Le Châtelier’s principle. Procedure: A diluted solution was made from the stock of 0.0025 M Fe(NO3)3 by diluting 4.0 mL to exactly 100.00 mL‚ using serological pipet and volumetric flask. The concentration of the diluted
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Anionic Exchange Ion Chromatography to Detect the Anions Concentrations in the water Samples Narendra Boppana‚ Nasser Ali‚ Martin Chavez‚ Sear Introduction: Ion Exchange Chromatography is a process where the ionic exchange occurs in between the stationary phase and the sample in the mobile phase‚ by using this ionic exchange phenomenon it is easy to separate the compounds. Ionic exchange depends on the electrical properties
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Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin‚ acetaminophen‚ ibuprofen‚ mefanemic acid‚ and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment‚ thin-layer chromatography (TLC) will be used
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Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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