Organic Lab I No. 5-6 Separation of a Carboxylic Acid‚ a Phenol‚ and a Neutral Substance (Two-week lab) Reading Assignment 1) Chapter 7 • Introduction: P. 135- first paragraph • Theory and Techniques (p142-145) o Properties of Extraction Solvents o Mixing and Separating the Layers o Drying Agents o Part 1: The Technique of Neutral Liquid/Liquid Extraction The description is for dichloromethane-aqueous
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Effects of Particle Size‚ Temperature‚ Concentration‚ and a Catalyst on Reaction Rate Introduction: Chemical reactions can be affected by a number of different factors. Particle size‚ temperature‚ concentration of a solution‚ and catalysts play a big role in the rate of reaction‚ they determine how fast a reaction will occur. According to the collision theory‚ the rate of reaction depends on the frequency of effective collisions between particles. Every reaction is different in that they all
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Student: Glavin Wiechert Group Member: Victoria Coe Due Date: May 2‚ 2011 Teacher: Louth‚ Ellen Mary Class: Chemistry 11 Ad Introduction: An empirical formula of a chemical compound is the ratio of atoms in simplest wholenumber terms of each present element in the compound. For example‚ Glucose is C H O ; it’s empirical formula is CH O. 6 12 6 2 A hydrate is a compound that is chemically combined with water molecules. In contrast‚ an anhydrate does not contain water
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Preparation of Standard Solutions and Use of a Spectrophotometer to Measure the Concentration of an Unknown Solution Introduction Concentration is the ratio of the quantity of solute to the quantity of the solvent or the quantity of solution. By knowing this‚ CO(NO3)2 *6H2O Purpose What is the percent transmittance of six different solutions of cobalt (II) nitrate hexahydrate using a spectrophotometer? Materials * Graduated cylinder (10 mL) * Graduated cylinder (25 mL) * pipet
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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Osha’s “Hazard Communication” for all existing chemicals that are currently sold. 3. What can make glassware unusable? Glassware is unusable in an experiment when it is chipped‚ broken‚ or cracked. 4. When should you wear goggles in the lab? You should wear safety goggles at all time. 5. How long should you wash your eyes if you get a chemical in them? If you have contact with chemicals in your eye‚ it should be rinsed for ten minutes. 6. What should you do if your clothing
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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Objectives: To use the process of chromatography to separate plant pigments. To compare the plant pigments found in spinach and red leaf lettuce leaves. Hypotheses: I believe the spinach leaf in the acetone will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. I believe the spinach leaf in distilled water will have a higher pigment of chlorophyll a and the red leaf lettuce will have a higher pigment of anthocyanin. Discussion Questions:
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able to experimentally analyze and isolate amino acids by separation techniques. One such method of separation is thin layer chromatography (TLC). In this method‚ a silica coated aluminum plate is spotted with the solution being separated‚ and the plate is placed in a developing jar containing a small amount of solvent. With time‚ the solvent
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SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification
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