BALANCE LAB Introduction The purpose of this lab is to become familiar with how to use the ChemLab program for determining mass using both the centigram balance and the electronic balance. Using the centigram balance‚ the mass‚ in grams‚ of a copper slug will be determined by both direct weighing and weighing by difference techniques. Using an electronic balance‚ the wight of a copper slug will be determined by also utilizing direct weighing and weighing by difference. Average mass of a
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Chemistry 111 – Lab #2 Formula and Composition of a hydrate Purpose of the Experiment: The purpose of this experiment is to provide an opportunity to practice proper heating and cooling techniques and to calculate the formula of a known anhydrous compound and to calculate the percent of water in an unknown hydrate from results. Materials and Methods: Materials used for this experiment included 3 crucibles (with lids)‚ clay triangle‚ ring‚ burner‚ CaSO4.2H2O and one unknown hydrate
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Purification of Recombinant Green Fluorescent Protein (rGFP) From E. coli strain‚ BL21(DE3)‚ Using Ni2+-Agarose Affinity Chromatography Abstract: The purpose of these series of experiments was to express and purify recombinant Green Fluorescent Protein (rGFP) from the E. coli strain‚ BL21(DE3) by beginning with its purification via a Ni2+-agarose affinity chromatography column. The His6 tag of the rGFP bound to the Ni2+-agarose column and washes and elutions were obtained‚ with elution 3 containing
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tissues ; Pipets‚ serological‚ 10-mL ; Sample of consumer sports drink‚ 10-mL ; Spectrophotometer ; Test tube rack Procedure: 1) Preeminently‚ follow all laboratory safety guidelines ensuring that you have safety goggles on at all times throughout the lab. 2) Fill the cuvette 2/3 of the way with water. 3) Select the appropriate wavelength on the spectrophotometer. 4) Place the cuvette in the spectrophotometer and click the calibrate button. 5) Put 5mL of stock solution into test tube. 6) Fill
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Name: Lab Partners: Experiment Date: 02/09/2013 Due Date: 02/23/2013 Lab Exercise No. 2 Determination of Kc Values Using UV Absorption Objectives * To determine the equilibrium constant for a given reaction * To understand the concept of Le Chatelier’s Principle * To gain experience in the use of a UV Spectrophotometer Background/Concepts * A chemical equilibrium is the state reached by a reaction mixture when the forward reaction and the reverse reaction
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Objectives: 1. To identify the relative densities of some common liquids and solids. 2. To determine the densities of water‚ an unknown liquid‚ a rubber stopper‚ and an unknown rectangular solid. 3. To determine the thickness of a piece of aluminum foil using the density concept. 4. To practice proficiency in performing the following experimental procedures: pippeting a liquid‚ weighing by difference‚ and determining a volume by displacement. Apparatus: Tall glass cylinder‚ glass marble‚ rubber
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Proportions. In our final lab‚ we took a sample of potassium chlorate and heated it in a crucible to drive off the oxygen. We did this to measure and find the amount of oxygen that was in it. This was to verify the Law of Definite Composition. Aspect 2: In our investigation of the Atomic laws we had some major results in all 3 experiments. In the Conservation of Mass Lab‚ we found that the total combined mass was equal to the original individual masses of the substances. In the Oreo Lab‚ our ratio result
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Encyclopædia Britannica Paper chromatography is used to separate "dissolved chemical substances". An example of dissolved chemical substances could be the pigments that make up a specific color‚ for example in a marker. When a chromatography paper experiment is done on a black marker‚ the experiment tends to separate all the different pigments that make up the color. In this experiment‚ the substance investigated was a black water soluble Giotto marker pen. Paper chromatography has two phases‚ the
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Gas Chromatography Analysis of Product Mixtures Gas Chromatography Guidelines‚ Handout. Introduction Gas chromatography is a technique used to analyze chemical compounds that can be vaporized and separated in a gas phase column. Once separated‚ the analyzed substance is passed through a detector and data is obtained. The samples that we are going to analyze are: the EtOAc from Simple distillation‚ the Fraction 1‚ Fraction 2‚ and Fraction 3 from the Fractional Distillation. Experimental Procedure
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Separation Of A Dye Mixture Using Chromatography ********* AP Chemistry (3rd Block) Fauquier High School 8/ 31/ 13 ABSTRACT: The Rf values of seven known color dyes and three unknown samples were observed in order to compare results and discover the hidden identities of the three samples. Three solvents‚ H2O‚ Isopropyl alcohol‚ and a 2% NaCl solution‚ were introduced to the dotted chromatography strips to show three different ratios for each dye on
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