Chem 31.1 Post Lab 3 CARBONYL COMPOUNDS AND CARBOHYDRATES HYDROLYSIS OF POLYSACCHARIDES - - Susceptible to both nucleophiles and electrophiles Presence of C=O TEST FOR ALDEHYDES AND KETONES - - Reaction with 2‚4-DNP (Dinitrophenylhydrazine) Positive: Formation of yellow precipitate IODOFORM TEST - Carbonyl compounds: Methyl ketones will give a positive result Postive: Yellow precipitate (CHI3) MOLISCH’S TEST for carbohydrates - TOLLEN’S TEST – for aldehydes
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Give brief answers to the following questions : 1. What happens to a molecule when it absorbs UV light ? rvv’ill occra’’ 6e cfr’oh fvhan ?- rr.ote c ulQ a$c‚ovb r4V t;g hl ‚ ?*rltdfi’oh o{ olQelraq ofc pronrottd potn *h’Qhr lraur1 dt *q‚fe to erc;feC‚ slst<‚Th<;6bSotb1 ) qh’:o&s ih n^olf c’‚la g r.in9 qb‚olal ob{‚1tr.ira :Lind c o1 et<cif l‚pln+t qt"d QqCh W-qrtr\ve 1t5 ou‚ 4iSfictlre ancvcry.0rl+yorvi6lst: qnI vrstblC t;Jht brin3 lboql rrrovc tbq\i v orl{rrro e.tCclro tts t5 Ricorlt5 fu o rv
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Majed Al Dhwaihi Chem 151 Lab Prelab Assignment for week 3 Chemical Separation Procedure: Separating the sample: First place a small amount of the spinach provided in the mortar‚ just enough to cover its bottom; combined with the sand provided to break down the call walls. A 1:1 mixture of hexane and acetone was determined to best solvent for this extraction. Knowing this add one mL of the pure hexane and one mL of the pure acetone to the mortar. Grind the contents until the cell walls
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Patel Chem 101 10-13-09 Introduction The objective of this lab was to show how stoichiometry is applied to a specific chemical reaction. Stoichiometry is pretty much the math behind chemistry. Doing stoichiometry can calculate masses‚ moles‚ and percents with a chemical equation. The use of stoichiometry is how we were able to find the limiting reagent in this lab. A limiting reagent is the chemical that will be used up first. Many calculations had to be made throughout this lab. Some
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Object Length (cm) Length (mm) Length (M) CD 12cm 120mm .12M Key 5.3cm 53mm .053M Spoon 15.5cm 155mm .155M Fork 19.8cm 198mm .198M Water Temperature C° Temperature F° Temperature K Hot from tap 38 C° 100.4 F° 311K Boiling 93 C° 199.4 F° 366K Boiling 5 minutes 95 C° 203.0 F° 368K Cold from tap 15 C° 59 F° 288K Ice water 1 minute 8 C° 46.4 F° 281K Ice water 5 minutes 5 C° 41.0 F° 278K Object Estimated mass (g) Actual mass (g) Actual mass (Kg) Mechanical pencil 4g 5
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S.4Chemistry 2nd Term Project Be a Chemical Analyst-Analysis on ethnoic acid. Objective This experiment aims at determining the percentage by mass of ethanoic acid(CH3COOH) in a commercial vinegar using titration method. Chemical reaction involved: CH3COOH(aq) + NaOH(aq)→ NaCH3COO(aq) + H2O(l) Apparatus: An electronic balance‚ a 10.0cm3 pipette‚ a 25.0cm3 pipette‚ a pipette filler‚ a 250.0cm3 volumetric flask‚ a burette‚ stand and clamp‚ a conical flask‚ a white tile‚ 3 large beakers‚
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Figure 1: Affinity chromatography of fumarase with the Ni2+-NTA-agarose column. Extract (9.9 mL) containing yeast (3.76g) in extraction buffer containing 0.1% Igapel CA-630 and protease inhibitors were pumped through Ni2+-NTA-agarose column. Fractions were collected by 1.5 mL portions by use of wash buffer (20.0 mL)‚ imidazole elution buffer (26.3 mL)‚ and wash buffer (10.0 mL)‚ again. Absorption readings were taken for all fractions with a Cary50 set at 280nm. The fumarase activity was determined
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Chromatography: How can we separate a mixture? Purpose The chromatography lab is to understand how molecules with similar molecular properties can be separated with paper chromatography. These differences will be interpreted to see the distinction of separate chemical substances. Pre Lab Questions 1. Explain capillary action as it pertains to water and paper. Capillary action makes water draw up the paper. As paper absorbs water mixes with the solutions in the paper. 2. What is the
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Investigating the Determining Characteristics of Cations and Anions Chem 111 Sec 560 Introduction: The purpose of this lab was to study the specific characteristics of cations and anions‚ and ultimately to be able to identify an unknown substance based on our studies and tests using the logic trees developed through the experiment. A logic tree is a graphical display of the findings from this lab which‚ through a series of yes/no questions‚ elimination tests
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Thin Layer Chromatography Introduction Thin Layer Chromatography or TLC is a technique used as a separation and identification technique. There are many forms of chromatography‚ but one thing that remains constant throughout all of the types of chromatography is that there is a stationary phase and a mobile phase. In the case of TLC the stationary phase is the silica gel on the TLC tray. Procedure Chromatograph method is a method of separating mixtures of two or more compounds. Two phases
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