The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Gempel September 3‚ 2015 Daphnia Lab Results Paper Abstract The goal of the study was to observe the effects of multiple chemicals on a Daphnia magna’s heart-rate compared to a control (pond water). The different chemicals were caffeine and alcohol. The heart-rate was the main variable in this experiment. The Daphnia’s heart-rate was observed for 15 seconds and then multiplied by 4 to show its heart-rate in one minute. This was repeated 4 times for each chemical. The data was averaged at the conclusion
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Presence of Alu element within PV92 on chromosome 18 in a mixed ethnical population and its connection with humans’ ancestral origins By Shiyu Song Biology 155Q LAB-A: 14:30-17:30 Dr. Jacob April 17th 2013 Abstract The Alu insertion (+) within PV92 on chromosome 16 usually reflects a person’s ancestral origin. This study aimed to find the correlation between the presences of Alu insertion (+) within PV92 on chromosome 16 within a population. We used PCR to detect the Alu insertions
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I) Introduction In this lab‚ a test was conducted to determine how the relative redox activity of chloroplasts from spinach leaves‚ which were performing photosynthesis‚ would change when in the presence or absence of light. To observe these changes in redox activity‚ the chloroplasts were exposed to DCPIP‚ a chemical that changes color according to such activity. By determining the redox activity of the chloroplasts‚ it could then be inferred which chloroplasts were photosynthesizing more actively
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Bio Lab Report Erica Patterson September 10‚2013 Intro to cellular and molecular Biology Lab Abstract: In the Biology Laboratory Manual by Darrell S. Vodopich and Randy Moore are results to a similar experiment. The studied the hypothesis of carbon dioxide production by yeast fed sugar is not significantly different than the carbon dioxide production by the yeast fed in protein. Their hypothesis is the one that has helped formulate ours. We also will be answering the same to questions “What
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Biofuel Enzyme Kit Katie Adamson Biochemistry Laboratory‚ BIO124L 1/29/15 Abstract The objective in this lab was to determine the effects different conditions had on the enzyme cellobiase. We examined reaction rates in the presence or absence of an enzyme‚ the effects temperature and pH changes on the enzyme and the effects enzyme concentration and substrate concentration had on the enzyme. As expected results showed us that cellobiase works optimally when conditions are favorable. We see this
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Photosynthesis is a form of a chemical reaction that transforms energy from the sun in to a chemical energy‚ a carbohydrate known as glucose. Only plants are able to use this process to make their own food. This chemical reaction takes place in Chloroplasts‚ cells that are found in any green part of the plant. Chloroplasts are in green fragments of the plants because it contains a green pigment called chlorophyll. Although Photosynthesis can occur in any green portion of a plant‚ it mostly occurs
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Termite Lab Report The effect of ink on termite behavior Abstract Termites produce special chemicals within their bodies called pheromones. Because termites are usually found inside dark areas‚ pheromones help them communicate in these places. Past studies have suggested that ink inside ballpoint pens contains pheromones‚ causing termites to follow any trail created by the ink. If a pathway is drawn with an ink pen‚ then a termite will be attracted to the path and trace it due to the pheromones
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Abstract An attitude is a representation of an individual ’s degree of like or dislike for an act‚ object or evaluation. This report aims to conclude whether there is a difference between female and male attitudes towards smoking. It is hypothesised that females will have a more positive attitude towards smoking. Questionnaires using a 5 point likert scale will be utilised to measure each participants’ attitude towards smoking through their responses. Results stated there is no significant difference
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Labs and Diagnostics Colonoscopy This test allows direct visualization of the small bowel‚ rectum‚ and colon. It is recommended for patients who have experienced fecal occult blood or obvious blood in the stool. It is also recommended for patients who have experienced a change in bowel habits or are experiencing abdominal pain. In patients who have had colorectal cancer‚ polyposis‚ or inflammatory bowel disease it is used to observe for continued progression of their condition. During the procedure
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