Material and methods Hosts ‚ plasmids and chemicals E. coli TOP10 strain was used for cloning and proliferation. E.coli BL21 DE3 was used as expression host cell. The pET 28a(+) plasmid was employed for gene expression. All chemicals were obtained from Merck Company (Germany). Codon optimization and gene synthesis Sequence encoding V-domain was obtained from Swiss-port‚ Uniprot KB and National Center for Biotechnology Information (NCBI) databases. 6x His-tag sequence was placed at N-terminal
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Lab Report: Toothpickase and Enzymes September 19‚ 2012 Olivia DePhillips Lab Partner: Joy Morgan Meyers Signature: Introduction Enzymes are biological molecules that catalyze chemical reactions. In enzymatic reactions‚ the molecules at the beginning of the process‚ called substrates‚ are converted into different molecules‚ called products. Almost all chemical reactions in a biological cell need enzymes in order to occur at rates sufficient for life. Like all catalysts‚ enzymes work
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Kiley O’Toole Lab Report 3 Purpose The purpose of this lab was to uncover the number of layers of zinc atoms around a piece of galvanized iron. This was done by pouring the hydrochloric acid onto the galvanized iron‚ thus removing the zinc from the piece of metal. Laboratory Procedure 1. Measured and recorded the mass of a piece of galvanized iron using a centigram balance and a ruler 2. Measured and recorded the length and width of the galvanized iron using the ruler 3. Put the
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Patrick Kelly 10/16/12 Physical and Chemical Changes Lab Question: How do you distinguish a physical and chemical change? Hypothesis: If we use these materials and use magnets‚ water‚ burners‚ and filters on the mixtures and elements given we should determine what kind of effect these materials given will have on these mixtures and elements‚ physical or chemical. Procedures: -Part A: Record all observations of Part A in Data Table 1 1. Label each substance on seven pieces of paper
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Lab Report 1 Introduction: Proper chemical formulas entitle many challenges such as the Law of Multiple proportions that states that there may be more than one plausible mole ratio for the elements in that compound. However if we determine the mass of each element in the compound we will be able to get the true chemical formula. In this experiment‚ we used the law of definite proportions to find the chemical formula for a hydrated compound containing copper‚ chlorine‚ and water molecules
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Course Number and Title: CHEM-181 General Chemistry I 2. Semester Hours: 4 Contact Hours: (3 + 2) Lecture Lab 3. Catalog Description This course‚ intended for science majors‚ is the first course of a two-course sequence. Course topics include stoichiometry‚ inorganic nomenclature‚ solutions‚ gas laws‚ thermochemistry‚ atomic structure‚ and chemical bonding. The laboratory work includes basic laboratory techniques and is intended to support lecture topics. It is highly recommended
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10/24/2013 LAB TITLE: Single Replacement Reaction of Solid Copper with Silver Nitrate PURPOSE The purpose of this lab is to: • Observe a single replacement reaction‚ and • Calculate the mole ratio of silver (Ag) to copper (Cu) in the reaction MATERIALS • Copper wire (30 cm) • Large test tube • 250 ml beaker • Silver nitrate (AgNO3) powder • Dilute AgNO3 solution • Distilled water • Electronic balance • Glass stir rod • Watch glass PRE-LAB DISCUSSION A single
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Liquid “X” and Liquid “Y” the same liquid? 5.What chemical reaction happened that made vapor during the combination of metal foil and Liquid “Z”? 6. What made the liquid turn darker in the combination of metal foil and Crystal “X” and Liquid “Y” solution? 7. What made up Crystal “X”? 8. Is Crystal “X” even a crystal? 9. What’s in metal foil that made it react to both the solution of Crystal “X” and Liquid “Y” and also Liquid “Z”? 10. What was this lab trying to teach us? 2. Write “educated guess”
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Bags of Reactions * Problem/Purpose * Background Information: The Law of Conservation of Mass was created by Antoine Lavoisier in the 18th century. This law stated that mass could matter could neither be created nor destroyed. During a reaction the bonds of the reactants are broken and form new substances. As stated in the Law of Conservation‚ matter can neither be created nor destroyed; because of this the products should have the same number and type of atoms as seen in the reactants
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Department of Chemistry Chem 180 Lab 5: Ionic Reactions Submitted by Abstract: The purpose of this experiment is to work with aqueous solutions of ionic substances. Aqueous solutions are those solutions in which water is the solvent. When ionic substances are dissolved in water‚ the ions separate and become surrounded by water molecules. The focus of this experiment is on precipitates. The goal of this experiment is to study the nature of ionic reactions‚ write balanced equations‚ and to
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