DISCUSSION In this experiment‚ we will extract plasmid DNA that carrying pKan and pAmp from E. coli that have been cultured overnight in LB media containing antibiotic kanamycin and ampicillin respectively. So‚ the DNA that will be introduced later will make the bacteria resistance to antibiotic kanamycin or ampicillin. A plasmid is a small‚ circular‚ double stranded DNA molecules and cloning vector that are widely used for recombinant DNA technology. It can be physically separated from chromosomal
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Chemical Reactions Lab Introduction Four different types of chemical reactions were observed in this lab. Synthesis reactions occur when two different elements or compounds form a more complex compound as shown by equation (1). Single replacement occurs when one element replaces another to form a new compound as shown in equation (2). Double replacement occurs when two different atoms in different compounds trade places with one another to form two new compounds as shown in equation (3).Combustion
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Chemical Reactions Lab Objectives: 1. To examine a variety of reactions including precipitation‚ acid-base‚ gas forming‚ and oxidation-reduction reactions. 2. To identify the products formed in these reactions and summarize the chemical changes in terms of balanced chemical equations and net ionic equations. 3. To identify the species being oxidized and reduced in oxidation-reduction reactions and determine which species is the oxidizing agent and the reducing agent. Chemical equations represent
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Affecting Reaction Rate Lab Report Objective: To observe and record the different effects of reactants on concentration‚ surface area‚ and temperature‚ on the reaction rates for each. Materials: Refer to the Experiment 18A worksheet Procedure: Refer to the Experiment 18A worksheet Data Table: Mass of 11cm strip of Mg: 0.13g Average mass of 1 cm piece of Mg: 0.0118 Table 1: Effect of Concentration on Reaction Rate Concentration of Acid Reaction Time (s) Reaction Rate (g Mg/s)
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Chemistry 1225 Lab Write-Up #13 Abstract Electrolysis is a method of using a direct electric current to drive an otherwise non-spontaneous chemical reaction. During electrolysis‚ hydrogen atoms (H2) are reduced at the cathode whereas the oxygen atoms (O2) are oxidized at the anode. There were three different solutions used in the experiment in order to have a better understanding of electrolysis reactions. From each solution‚ reaction equations were produced. The reactions could be observed from
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College Department of Chemistry Chem 180 Lab 5: Ionic Reactions Submitted by Abstract: The purpose of this experiment is to work with aqueous solutions of ionic substances. Aqueous solutions are those solutions in which water is the solvent. When ionic substances are dissolved in water‚ the ions separate and become surrounded by water molecules. The focus of this experiment is on precipitates. The goal of this experiment is to study the nature of ionic reactions‚ write balanced equations
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Light Reactions Lab This lab explores the effect of light intensity and light wavelength on photosynthesis. The quantity of energy [ATP] produced will change depending upon on these parameters. Use "Johnson Explorations: Photosynthesis" located at http://www.mhhe.com/biosci/genbio/biolink/j_explorations/ch09expl.htm Examine the diagram before clicking "skip intro" so you have an idea what is going on. The animation show the movement of photons‚ electrons‚ water‚ protons‚ ATP etc. relating to
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In this lab‚ the pH of flat sprite and a fresh sprite are compared two ways: one way was to record the pH directly with a digital pH probe and the other was to calculate the concentration through titration. Because the reaction is a neutralization reaction‚ the concentration of can be calculated if the concentration of is known. At the end of the titration‚ the moles of will equal the moles of and the pH is expected to be greater than 7 because the found in sprite is weak and is a strong
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Abstract The separation of a Fe3+ and Ni2+ mixture was firstly investigated; followed by the determination of the Fe3+ and Ni2+ content (concentration) in the original mixture. It was found that iron was a yellow solution and nickel respectively had a greenish colour. Their original concentrations respectively were 0.03669 M for iron and 0.03159 M for nickel. Introduction Ion exchange materials are insoluble substances containing loosely held ions which are to be exchanged with other ions in
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of steel wool into the test tube and measured the temperature again. We stored the test tubes on the test tube rack to observe the next day. A chemical reaction did occur in the lab. There were a color change and an odor change that was observed on Day 2 of the lab. On Day 1‚ the liquid was clear and the smell was very strong. On Day 2 of the lab‚ most of the liquid was gone but the color was a muddy color and the smell got milder‚ it wasn’t as strong as Day 1. Not only was there a color and odor
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