University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
Premium Protein Chromatography Analytical chemistry
Hugh Kim Lab Report: Stoichiometry Lab 1. Prelab Part1. 1) Create no waste = The principle that encourages chemists to not create waste at the first place rather than cleaning it up afterwards effectively shifts the chemistry more environmentally conscious‚ as creating no waste would make the experiment efficient; the reactants will be reduced to only the essential ones and the product will be maximized‚ a change that would make the experiment economic. Also‚ if chemists aim to
Premium Chemistry Chemical reaction Hydrogen
This lab has introduced the use of daphnia‚ pill bugs‚ amoeba and Uglena. We the AP Biology students were first introduced to the test subjects to test his or her capability of handling living organisms for upcoming science experiments. The AP Biology students were asked to create original experiments for all test subjects testing reactions to gravity‚ light intensity and reaction to food. Daphnia‚ or Daphnia magna‚ are microscopic organisms in which the students created an original lab to test
Premium Cell Microorganism Bacteria
Laboratory 6: Energy Changes in Chemical Reactions Note: Lab reports are to be completed by each student individually and in their own words Observations: (This part is to be completed in class) Part 1: Physical Change- Heat of Solution for Sodium hydroxide (NaOH) 3.2 grams NaOH Result: Temperature of DI water = ____22.8 °C Temperature after addition of NaOH = ______31.8 °C Part 2: Chemical Change- Reaction between acid and base Result: Temperature of HCl = ___21.4 °C Temperature after addition
Premium Thermodynamics Chemical reaction Energy
Solute Concentration of Potatoes Lab #1 Purpose: To find the molarity/concentration of potato cytoplasm. Materials: As on page 1 in the lab handout. Procedure: As on page 1 in the lab handout. Data and Observations: Test Tube # | Concentration of sucrose solution (mol/L) | Initial Mass (g) | Final Mass | Percentage change in mass | 1 | 1.0 mol/L | 3.00g | 2.25g | -25.0% | 2 | 0.9 mol/L | 2.70g | 2.07g | -23.3% | 3 | 0.8 mol/L | 2.92g | 2.25g | -22.9% | 4 | 0.7 mol/L | 2.60g
Premium Concentration Osmosis Chemistry
Willy Rupprecht Date(s) performed: October 2 - 3‚ 2012 Gravimetric Analysis of an Unknown Group 1 Metal Carbonate Purpose: The purpose of this lab is to determine the identity of a Group 1 metal carbonate compound by gravimetric analysis. Procedure: The unknown carbonate is weighed and then dissolved in water. A solution of CaCl2 (calcium chloride) is added to the metal carbonate solution to precipitate the carbonate ions as CaCO3 (calcium carbonate). The precipitate is then filtered‚ dried
Premium Calcium Filter paper Gravimetric analysis
Evelyn Ramos CHEM LAB 201-003 Professor Ruddock Experiment #4 - Chemical Reactions Performed: 3/6/13 DUE: 3/13/13 Pre Lab Questions: 1. Before you can write a chemical equation‚ what must you know? You have to know what the products and reactants are. 2. What observations might you make that suggest that a chemical reaction has occurred? If a gas is produced or precipitates are formed that indicates chemical reaction. Also‚ if color changes occur or
Free Solubility Chemical substance Chemical reaction
Abstract This lab’s purpose was to test the patterns of percolation in various places. This lab tested how soil would absorb water based on different places. Distance from a group of trees‚ distance from a swamp‚ and different types of soil were tested. From the results collected‚ you can not prove‚ but you can infer that the ability to absorb increases as you go farther away from a swamp‚ and a group of trees. The ability to absorb also increases based on how much silt is in soil. Introduction
Premium Soil Surface runoff Digestion
The Charge/Mass (e/m) Ratio of the Electron PHYS 0212: Introduction to Laboratory Physics Fall 2012 Abstract The experiment conducted demonstrated correlation between the charge and mass of an electron and the behavior of magnetic fields. The lab was divided into four parts. The first three parts were conducted with a compass that was used to locate the magnetic field lines around a bar magnet‚ a solenoid and then a pair of Helmholtz coils. As a result of these trials‚ our observations found
Premium Magnetic field
Chloride Scarlet Red Ni+2 Dark Orange Copper Sulfate (solid) Green Fe+2 No Reaction Sn+2 Orange Zn+ Orange LiCl Magenta KCl (solid) Smoky Purple 1. The inaccuracies that may be involved in using flame tests for identification purposes is that there are certain ions that produce the same flame color and further identification must be made to identify the substance. 2. The pairs of ions that produce similar colors are K2CR2O7 and NaCl‚ Sn+2
Premium Photon Atom Electromagnetic radiation