Chromatography lab Purpose: To separate food colorings into their component dyes using paper chromatography. Materials: Chromatography paper‚ Food coloring‚ Ruler‚ Pencil‚ Solvent solution‚ Test tubes‚ Test tube rack. Safety precaution: wear aprons‚ to make sure that you don’t get any of the alcohol on your clothes‚ and if you break a test tube you don’t get glass on you. Procedure: See-attached handout. Results: See chromatography with Audrey’s lab report.
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of proteins in living systems. It is used to examine when and where proteins are being expressed‚ rate of degradation‚ modifications‚ how they interact with other proteins and more. The goal in this lab was to determine the similarities and differences in muscle proteins among 5 species of fish using SDS-PAGE and Western blotting. First‚ proteins are separated based on size. Second‚ antibodies are used to detect the protein of interest. Lastly‚ a substrate that responds with an enzyme is used to
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rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water‚ PO4 buffer‚ and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer‚ measuring the greatest change in absorbance. From
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Introduction The aims of the lab session was to familiarize with the principles‚ operation and application of a light spectrophotometer. This report discusses an experiment to study the relationship of absorbance and concentration‚ the interaction of electromagnetic radiant energy(ERE) and matter which is an important aspect of the Beer-Lambert ’s Law. This law states that the absorption of a compound is directly proportional to the concentration of that particular compound. This is because (a &
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integral part of every job and not a separate entity. In this document‚ only health and safety aspects will be considered. The terms "job" and "task" are commonly used interchangeably to mean a specific work assignment‚ such as "operating a grinder‚" "using a pressurized water extinguisher‚" or "changing a flat tire." JHAs are not suitable for jobs defined too broadly‚ for example‚ "overhauling an engine"; or too narrowly‚ for example‚ "positioning car jack." What are the benefits of doing a Job Hazard
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Lab 2.6.2: Using Wireshark™ to View Protocol Data Units Learning Objectives • Be able to explain the purpose of a protocol analyzer (Wireshark). • Be able to perform basic PDU capture using Wireshark. • Be able to perform basic PDU analysis on straightforward network data traffic. • Experiment with Wireshark features and options such as PDU capture and display filtering. Background Wireshark is a software protocol analyzer‚ or "packet sniffer" application
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Stoichiometry Lab Stoichiometry is the end result of adding up chemical elements that were involved in chemical reactions (http://dictionary.reference.com/browse/stoichiometry). The word stoichiometry was obtained from two greek words meaning element and measure. This explains the definition for the term. A chemist named Jeremias Benjamin Richter was the chemist responsible for first realizing what stoichiometry was (http://www.chemteam.info/Stoichiometry/What-is-Stoichiometry.html). In 1972
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not exist (Lab Manual 3 pg. 1). They help in many different ways that are useful to the body of living organisms. Enzyme are used to speed up chemical reactions (Lab Manual 3 pg. 1). Through this process‚ they are considered very unique because they are not altered or consumed within the reaction (Lab Manual 3 pg. 1). This is why enzymes are considered biological catalysts. They also do not alter the equilibrium of a chemical reaction nor the amount of free energy that is released (Lab Manual 3 pg
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Synthesis of Butyl Benzoate Using Phase Transfer Catalysis The objective of the experiment is to synthesize the butly benzoate by nucleophilic substitution and characterize it by IR spectroscopy. The percent yield of the final product is determined after the synthesis. Procedures: 2.0 mL of 1-bromobutane‚ 3.0 g of sodium benzoate‚ 5.0 mL of water‚ 4 drops of Aliquat 336‚ and a boiling stone were placed in a 50mL round-bottomed flask. The reaction mixture was refluxed for 1 hour and the flask
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Purpose: To observe epithelial tissue by using a microscope to compare prepared slides. Introduction: Epithelial tissue covers the whole surface of the body. It is made up of cells closely packed and ranged in one or more layers. This tissue is specialized to form the covering or lining of all internal and external body surfaces. Epithelial tissue can be divided into two groups depending on the number of layers of which it is composes. Epithelial tissue which is only one cell thick is known as
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