General Biology I‚ Fall 2011 Lab Report 3 Name: Luan Nguyen Date: 11-30-11 Lab: Gel Electrophoresis Purpose of this lab: In this laboratory investigation‚ students will analyze hypothetical human DNA using restriction endonucleases and gel electrophoresis to match samples from a crime scene to a suspect. Introduction: Your analysis unit will use gel electrophoresis to determine the guilty party. Electrophoresis is a technology in science that allows an individual to separate
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Sample Questions 1. In the chemical formula for ammonia‚ NH3‚ what does the subscript 3 represent? (a) the number of ammonia molecules that will bond together (b) the number of nitrogen atoms in each molecule of ammonia (c) the number of nitrogen and hydrogen atoms in each molecule of ammonia (d) The number of hydrogen atoms in each molecule of ammonia 2. The diagram above shows a block from the periodic table. The number six represents the following consumers eats only producers? (a) atomic
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of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results. II. Introduction: The objective is to determine the mass percent of iron in an iron compound using a spectrophotometer. From there‚ determine which iron compounds are in the stock room bottles based off of the experimental mass percent results. The objective is going to be met by first using absorption spectroscopy. This
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the SDS-PAGE and Simple-PAGE gels were prepared with 3.25mL water‚ 2.0mL 4X resolving buffer‚ 2.7mL 40% acrylamide solution‚ 80μL 10% APS‚ and 3μL TEMED. The stacking layers for the SDS-PAGE and Simple-PAGE gels were prepared with 4.6mL water‚ 2.0mL 4X stacking buffer‚ 1.3mL 40% acrylamide solution‚ 48μL 10% APS‚ and 5μL TEMED. The SDS-PAGE gel used the buffers containing SDS and the Simple-PAGE gels used the buffers without SDS. The gels were run at 100 V for about 60
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Abstract: Bacteria consists of a large domain of prokaryotic microorganism in which can fight of antibiotics allowing the bacteria to become antibiotic resistant. In this lab report‚ we discover the most effective disinfectant that would work best in killing the harmful bacterial strain‚ Bacillus subtilis. KB testing or disc diffusion antibiotic sensitivity testing is measured through the diameter in millimeters to find how resistant the antibiotic to the bacteria. The hypothesis of Windex fell correctly
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during the lab session Procedure Part A This lab was performed by the group members as they closed both low and high flow rotameters (Fig-2) and filled the tank with water. Then‚ the large rotameter was opened when the water in the tank had reached the internal baffle‚ so the flow from the tank was reduced by doing so. One member was in charge of adjusting the level of the water so it was always maintained over the baffle’s level during the lab (Fig-1). The first part of the lab was done
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CO2 Lab Report Intro Alka-Seltzer tablets are used to treat cold/flu symptoms as it produces CO2 when dissolved in water. The goal of this lab is to calculate the amount of NaHCO3 is needed to produce enough CO2 gas to fill the room. Experimental One Alka-Seltzer tablet was weighed on the analytical balance. Then a piece was measured out to be between 0.21 and 0.28 grams. 500mL of distilled water was added to a 600mL beaker. 80mL of water was mixed with 20mL of 6M HCl in a 150mL beaker. A
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A company has specified that the acceptable size of a knob that is to be produced is (0.140 ± 0.003) inches. In this lab‚ the dimensions of the knobs produced in one day are tested for discrepancy in order to pass control testing. This lab also reports the probability that knobs with the acceptable dimension will be produced. MATERIALS AND METHODS Using Microsoft Excel‚ a histogram was created displaying the three documented dimensions of 15 batches of knobs. This histogram was obtained in order
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Anxiety Lab Report Abstract: The aim of this study is to investigate the relationship between cognitive anxiety‚ somatic anxiety and self confidence in two different situations; non-stressful and stressful. It is to examine the effects on anxiety of a simple golf putting task. The stressor used in this experiment can be described as situational‚ namely the stressor of social comparison‚ the type of stressor that leads many performers to question their own ability which in turn evokes symptoms
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between the amount of substrate in the assay solution and the rate of the reaction when the enzyme and buffer in the assay are held constant were experimented. We analyzed the change in absorbencies over time for varying substrate concentrations. There were four experimental assays which contained 1% enzyme solution‚ substrate solution of 0%‚ 1%‚ 2%‚ and 3% concentrations‚ guaiacol‚ and pH 7 buffer. At 2% concentration there was a greater enzymatic activity and at 3% concentration enzymatic activity
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