In this lab we tested the effect of temperature has on the rate of enzyme activity. The way we figured this out was by taking four different temperatures and testing the difference absorbance levels they produced every 20 seconds for about 2 minutes straight using a spectrophotometer. The important part of this experiment was the temperature the enzyme concentration was made at. What we got from the experiment was at lower temperature we got very low numbers for the absorbance‚ which gave us a lower
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Enzymes Reactions to Changes in Substrate and Inhibitors Benjamin J. Mora Coronado University of Texas Rio Grande Valley at Edinburgh Abstract Purpose for the experiments was to test the enzymes in various scenarios and see how changing this would affect the rate of reaction. The enzyme source used in the experiments was Turnip Extract. Concentrations of Turnip extract for activity 1 where o.5ml‚ 1.0ml‚ and 2.0 ml as for the rest of the activities 2 Through 4 stayed at a consistent concentration
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Lab #5-Enzymes NAME DATE LAB PERIOD Introduction Enzymes are proteins‚ though highly complex and diverse‚ they serve one basic function; to work as an organic catalyst. A catalyst‚ as defined by Merriam-Webster dictionary‚ is a substance that enables a chemical reaction to proceed at a usually faster rate ("Catalyst-Definition and more."). They function by reducing the activation energy‚ or energy required to start a reaction. The way enzymatic reaction works cannot be altered‚ but the
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The objectives of this experiment were to investigate the activity of enzymes‚ components that influence the enzyme’s activity‚ identify an unknown phosphatase‚ influence of inhibitors‚ and determine if inhibition is competitive or noncompetitive. A spectrophotometer evaluated the measurement of color change over a period time due to product being formed. Determining unknown phosphatase and effects from different inhibitors were determined by varying the pH and substrate concentrations. The unknown
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Reminder: All post labs need to be 1- typed (not handwritten) ‚ 2- original (not copied from a classmate)‚ 3- answered using complete statements and 4- turned in at the beginning of the lab. Post-lab questions for Topic 5 – Enzymes Name: Date: Group: T W R Formation and Detection of Benzoquinone Table 1. Formation and Detection of Benzoquinone: Record Absorbance Time 2A-Potato extract + cathecol 2B- Potato extract + water 2C- Catechol + water After 10 min 1- What were the substrate
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Chemistry 512 Enzyme Catalysis Lab Report Pre-lab Questions: 1. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase. 2H2O2 2H2O + O2 (4H1 4O) (4H + 2O + 2O) 2. What is the substrate(s) of this reaction? What is the catalyst? Substrate = H2O2 hydrogen peroxide Catalyst = peroxide 3. At what approximate temperature do enzymes normally operate in the body of a warm-blooded animal? Would your answer change if the enzyme came from a plant or yeast? Enzymes normally
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regular hydrogen peroxide 1%‚ 25%‚ 50%‚ 75%‚ and 100% substrate concentration tweezers timer permanent marker pen/pencil paper for data PROCEDURE: The experiment begins with five beakers full of various substrate concentrations. To begin the lab‚ gather five plastic‚ Solo cups and label them 1%‚ 25%‚ 50%‚ 75%‚ and 100% with the permanent marker. Then‚ fill each cup with 75 mL of regular hydrogen peroxide. After‚ lightly dip the filter paper into the 1% substrate concentration till it is completely
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BIOL 1364 LABORATORY EXERCISE 3 ________________________________________________________________________ INHERITANCE OF CHARACTERS Objectives of the Laboratory: i) Determination of the genetic control of seed colour‚ ie how many genes control seed colour‚ what sort of intra allelic (dominance relationship) and interallelic interactions (independent assortment vs epistasis) govern the inheritance of seed colour. ii) Understand the scientific process involved in studying character
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Stallings Period 2 January 26‚ 2013 Enzyme Catalysis Lab Report Background: Enzymes are catalyst‚ which affect the rate of a chemical reaction. One consequence includes the cell to carry out complex chemical activities at relatively low temperatures. In these reactions the substrate binds reversibly to the active site. The cause of this is a decrease in the energy needed to activate the reaction of the substrate molecule to from products. Every enzyme is particular for a reaction for the reason
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Title: Enzyme Activity Lab Purpose: To measure the rate of enzyme activity from a tissue abstract and experiment with different factors‚ such as the enzyme solution and the substrate with different hydrogen peroxide percentages and temperature‚ that affect enzyme activity. Hypothesis: 1) If the disk is placed into each beaker with 100 units/ml of enzyme solution‚ then the time for the disk to float will be 30 seconds. 2) If the temperature of the solution is at 5 degrees Celsius‚
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