Cell Membrane Developers of Lab: Adapted by James Kirby JD726‚ Jennifer Mortellaro JD449‚ and James Prockup JD575 from a publication by the Department of Biological Sciences at Western Michigan University‚ Kalamazoo‚ MI Overview of Lab: Description: The purpose of this lab is to illustrate the effects that temperature and solvents have on the cell membrane. Red beet tissue contains large amounts of betacyanin‚ a red pigment‚ located in the large internal membrane vacuoles
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beet cell membranes Introduction: Cell membrane is the plasma like substance that separates different organelles and molecules within an organism. The cell membranes vital function is to permit passage of protein and enzymes through their wall. Red beets‚ also known as Beta Vulgaris has a red pigment that is located in its large central vacuoles. It is my hypothesis that if the cell membrane is to experience extreme temperatures‚ then it’s most likely it will affect the membrane. * I hypothesized
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Investigating the Effect of Temperature on the Permeability of Membranes The permeability of membranes can be altered in several ways. From previous preliminary work I know that reagents‚ for example detergents and alcohol alter the arrangement of the phospholipids in the membrane allowing substances to leak out of the cell. Temperature also affects the membrane. As membranes relay on protein molecules to allow molecules to enter and leave the cell through facilitated diffusion the temperature must
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TRANSPORT ACROSS MEMBRANE define diffusion and describe its role in nutrient uptake and gaseous exchange in plants and humans define osmosis and describe the effects of osmosis on plant and animal tissues define active transport and discuss its importance as an energy-consuming process by which substances are transported against a concentration gradient‚ as in ion uptake by root hairs and uptake of glucose by cells in the villi . A cell is surrounded by a layer of cell surface membrane. This phospholipid
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The effect of temperature on the cell membranes of beetroot cells and amount of pigment released. Apparatus • Corer size 4 • White tile • A Beetroot • Automatic Water Bath • Segregated knife • A thermometer • Stopwatch Method: • First take the white tile and the corer. Then collect a cylinder of beetroot by pushing the corer into the beetroot and withdrawing it. The cylinder remains inside the corer- so push it out with the end of a pencil. • Collect 3 cylinders
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Practical Assessment Planning AIM Beetroot cells contain a red pigment‚ which is stored in the cell vacuole and a vacuole membrane to prevent this leaking out of the cell surrounds it. The outer of the cell is also surrounded by a membrane‚ which again helps contain the pigment inside the cell. In this experiment I aim to find out the relationship between the leakage of red pigment from a beetroot cell and the surface area. To do this successfully I will need to alter the surface area of the beetroot
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Purpose: Examine the role of the cell membrane in the cell by disrupting its function using temperature (Biology 107 Laboratory Manual 2014). This will improve the general understanding of optimal growing temperatures and the breakdown of the cell membrane Procedure: Betacyanin solution of a known concentration was diluted to create a dilution series‚ then placed in a spectrophotometer set to 525 nm. The absorbance of the dilution was used to create a standard curve for betacyanin. Discs of living
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Chapter 6 Resting Membrane Potential (RMP) What is the Resting Membrane Potential (RMP)? ELECTRIC POTENTIAL – separation of opposite charges between 2 points. (“Has the potential to do electrical work if allowed to come together!”) vs. large potential difference small potential difference Magnitude of charge separation = potential difference ** measured in volts (1 Volt
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Outline the roles of membrane at the surface of the cell The plasma membrane encloses the cell‚ defines it boundaries and maintains the essential differences between the cytosol and the extra-cellular environment. The separation of the internal and external environment maintains a constant internal environment which is essential for the cell to function efficiently. Membrane receptors can interact with specific molecules corresponding to stimuli such as hormones to generate a signal cascade which
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across the cell membrane is that the carbon dioxide molecules spread out rapidly out your red blood cells down their concentration gradient to your lungs. When water molecules move freely through your cell membrane is called osmosis as we learned last unit. The reason why molecules move across the cell membrane is because they are small molecules that have no charge like oxygen which the cell membrane need to survive. So basically the molecules that move across the cell membrane are necessary to
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