Alyssa Caparelli Organic Chemistry 12A Professor Alston October 28‚ 2014 Isolation of Chlorophyll and Carotenoid Pigments from Spinach Purpose The purpose of this experiment was to isolate ß-carotene‚ chlorophyll-A‚ and chlorophyll-B from spinach using column chromatography. Spinach was dehydrated using ethanol‚ and the pigments were extracted with dichloromethane. The spinach extracts were dried using CaCl2. Then‚ the solid pigments were run through a column using a non-polar solvent‚ hexane
Free Solvent Acetic acid Ethanol
of Chlorophyll from Fresh Spinach and Investigation of the Photochemistry of Chlorophyll Chlorophyll a Chlorophyll b E5 - Extraction of Chlorophyll from Fresh Spinach E6 - Investigation of the Photochemistry of Chlorophyll The aim of this experiment is to investigate the photochemistry of chlorophyll. This experiment will be performed in two lab periods. In the first lab period you will extract chlorophyll‚ the green pigment in leaves‚ from spinach. In the second lab period
Premium Chlorophyll Photosynthesis Chromatography
COMMENT 343 DETERMINATION OF CHLOROPHYLL AND PHEO-PIGMENTS : SPECTROPHOTOMETRIC EQUATIONS~ It has been shown that chlorophyll degradation products may at times constitute a significant fraction of the total green pigments present in seawater (Yentsch and Menzel 1963; Lorenzen 1965; Yentsch 1965). These degraded forms‚ or inactive chlorophyll‚ absorb light in the red part of the spectrum; if they are present in concentrations significant relative to chlorophyll a‚ a serious error may be introduced
Premium Chlorophyll Spectroscopy Chromatography
contain many pigments such as chlorophyll b‚ xanthophyll‚ and beta carotene besides chlorophyll a. They are part of the photosystem in the thylakoids. Pigment molecules can be separated by a procedure called chromatography. The procedure uses a special paper and solvent. The chlorophyll molecules adhere to the paper. The solvent molecules move up the paper by capillary action. Each chlorophyll molecule will travel up the paper at different rates. Hypothesis: If chlorophyll is removed from the plant
Premium Chlorophyll Photosynthesis
for animals that eat animals that eat plants" for animals that eat animals that eat animals that eat plants‚ etc. (Levine 726) All humans rely on these plants to produce oxygen from photosynthesis. The presence of light and of the green pigment chlorophyll makes the change of carbon dioxide and water to glucose and oxygen possible. It was Jan Ingenhouz with Jean Senebier in Geneva that founded the basic gist of the theory of photosynthesis in plants. My experiment involves testing different brands
Premium Chlorophyll Photosynthesis
Chromatography paper is able to separate mixed pigments due to their polarity and solubility. Pigments of chlorophyll a‚ chlorophyll b and beta carotene will be separated on chromatography paper because each has its own polarity and solubility‚ which results in different distance traveled up the paper. Beta carotene is non-polar so it travels the highest distance‚ followed by chlorophyll a. Chlorophyll b is the most polar; therefore‚ it travels the shortest distance. The separated pigments on the chromatography
Premium Color Chemical polarity Chromatography
of senescence when the breakdown of chlorophyll exceeds a chlorophyll production. The leaves of these trees appear to change to yellow and oranges. Using your knowledge of photosynthetic pigments‚ explain the source of these yellow-orange hues. The carotenoids‚ which are the pigments that reflect in the yellow to red spectra and absorb blue light. There are some‚ functioning as secondary pigments involved in photosynthesis‚ that is unmasked once the chlorophyll content diminishes. 3. Duckweed is
Premium Chlorophyll Photosynthesis Visible spectrum
underground‚ the stimulus of not having any sunlight prevents the plants from growing chlorophyll and thus from growing leaves. SInce no sunlight is present underground‚ growing leaves would be pointless and would stunt the very essential growth of the plant as well as wasting very precious resources. Therefore hormones in the plant signal the formation of proteins that inhibit production of chlorophyll and promotes extensive growth‚ ensuring that the plant will reach sunlight as soon as possible
Premium Week-day names
CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
Premium Laboratory Experiment Stanford prison experiment
Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
Premium Critical thinking Thought