Photosynthesis Lab Data Collection: The absorption of different wavelengths of light by Chlorophyll Wavelength (nm) Absorbance of light by chlorophyll (Arbitrary units) Diluted Calculation New Reading (Arbitrary units) Violet 430 2.35 50% 2.35 x 2 3.20 Blue 470 1.09 - - 1.09 Blue-Green 492 0.38 - - 0.38 Green 520 0.77 - - 0.77 Yellow-Green 550 0.85 - - 0.85 Yellow 580 1.43 - - 1.43 Orange 600 0.65 - - 0.65 Red 700 0.16 - - 0.16 Absorption and reflection of light: Different
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Investigation into the photosynthetic activity of isolated chloroplasts from spinach beet to various light conditions. Introduction Chlorophyll‚ an important pigment in chloroplasts‚ is an essential component in photosynthesis; converting electromagnetic radiation from the sun into potential chemical energy. Photons excite electrons in chlorophyll which move through the electron transport chain‚ resulting in energy being stored in the bonds of ATP and NADPH. The flow of electron through the electron
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of life‚ cells. Within the plant cell‚ the dying off of chlorophyll causes the different color changes. Plants are autotrophs. Autotrophs are living things that make their own food. Plants make their food within their cells by using sunlight‚ and chlorophyll. This process of plants making their own food with sunlight and chlorophyll is called photosynthesis. The photosynthesis process involves sunlight‚ sugar‚ and chlorophyll. Chlorophyll is called a photoreceptor. It is found in the chloroplasts
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Results In this experiment we used paper chromatography to determine the amount of chlorophyll a‚ chlorophyll b‚ xanthophyll‚ and beta-carotene in a tube of blended leaf extract. We recorded our findings on the table labeled table 1. Table 1 shows the transmittance at each wavelength on a table from 400 to 720. The information on Figure 1 came from the leaf extract on the paper chromatography that we used; with the help of acetone we saw the spectrum and the differences of the different pigments
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Chloroplasts Chloroplasts are food producers of the cell and they make the plant green. They are organelles found in plant cells and animal cells do not have chloroplasts. Also‚ they have a eukaryotic alga that conducts photosynthesis. In a plant cell there can be anywhere from 10 to 100 chloroplasts in one cell. They are approximately 4 to 6 cm in diameter and shaped like a satellite dish with the concave face forward toward the light. Also‚ they are similar to mitochondria but mitochondria works
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found in plants is chlorophyll. This pigment exists in two forms; chlorophyll a and chlorophyll b. Chlorophyll a‚ being the main photosynthetic pigment‚ has a primary purpose to convert light energy to chemical energy used by the plant itself. Chlorophyll b absorbs light in a region of the spectrum apart from the dominant chlorophyll‚ and transfers the energy it produces to chlorophyll a. Along with chlorophyll b in transferring their energy produced to the dominant chlorophyll‚ two other pigments
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dioxide and water. Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a byproduct. It produces sugar and other organic compounds such as lipids and proteins. The sugars are then used to provide energy for the organism. Light reaction is the series of biochemical reactions in photosynthesis that require light energy that is captured by light-absorbing pigments; such as chlorophyll‚ to be converted into chemical energy in the form of ATP and NADPH. Light reactions
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pigments‚ if chlorophyll a absorbs blue-violet light‚ chlorophyll b absorbs blue light‚ carotene absorbs blue-green light‚ and xanthophyll absorbs blue light‚ then wavelengths of greatest absorption will be 400‚ 450‚ 500‚ and 450 respectively. Independent Variable: Wavelengths Dependent Variable: optimum wavelengths of light for absorption Standard Variable: method of measurement‚ what was measured Predictions: Chlorophyll a absorbs best at a wavelength of 400‚ Chlorophyll b at 450‚ carotene
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_DATA PROCESSING_: Coloured band Pigment Ratio of fronts Green Chlorophyll _b_ 0.8961 Yellow Carotenoid 0.9615 _EVALUATION_: Study Questions: One of the requirements for the photosynthesis process to occur‚ the plant cells need sufficient light. Pigments are the substances that absorbed visible light. Different pigments absorb light of different wavelengths. From the absorption spectrum of chlorophyll a‚ it suggests that blue and red light work best for photosynthesis. There
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Discussion: The effect of wavelength of light on the rate of photosynthesis was investigated in this experiment. The results from Figure 13 suggest that white light‚ containing all wavelengths of coloured light‚ has the highest rate of photosynthesis. Purple light had the second highest efficiency‚ followed by blue‚ red‚ and yellow light. Green light was significantly less efficient‚ producing the second lowest pH reading (Figure 14). The samples exposed to no light had the lowest rate of photosynthesis
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