CHM130 Lab 9 Chromatography Name: Karlee Rose A. Data Table (12 points) Paper # Color Source Solvent Distance Component Moves Distance Solvent Moves Rf value 1 Yellow M&M Candies 0.1% Salt Solution 28.88mm 42mm 0.69 2 Yellow Reese’s Pieces 0.1% Salt Solution 16.95mm 32mm 0.53 3 Purple Grape Soda 0.1% Salt Solution 32.15mm 51mm 0.63 4 Purple Grape Koolaid 0.1% Salt Solution 12.12mm 31mm 0.39 5 Red Easter Egg Dye 0.1% Salt Solution 1.18mm 7mm 0.17 6 Red Dry Erase Marker
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Littlefield Labs 1 Capacity Management at Littlefield Labs I. Introduction There are 3 stations in the game called sample preparing‚ testing‚ and centrifuging‚ while there are 4 steps to process the jobs. Before the game started‚ we tried to familiarize with the process of the laboratories and calculating the costs (both fixed and variable costs) based on the information on the sheet given. We did not intend to buy any machines too early‚ as we wanted to see the demand fluctuation and the
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components are altered. In this lab experiment‚ we will be doing an in-depth research of exactly what happens to the enzymes‚ when it happens‚ and why it denatures the way it does. b. Hypothesis Materials and Methods a. Materials 50ml beaker of fresh potato catalase Reaction chamber Ring stand and clamp 10ml graduated cylinder 100ml graduated cylinder 3% hydrogen peroxide Pan (water bath) Pipette Hot plate Ice Thermometer Boiled Catalase Buffers of varying pH: 4‚7‚10 Distilled
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Since the Grignard reagent can easily react with water‚ all glassware including the 25 ml round bottom flask‚ magnetic stir bar‚ 3 and 5 ml conical vial‚ 50 mL Erlenmeyer flask‚ claisen adapter‚ drying tube and 5 glass pasteur pipets were first added to a 250mL beaker and placed in the oven for 30 minutes. After the completion of the thirty minutes‚ 0.150 g of shiny magnesium turnings and a stir bar was first added to the round bottom flask and the claisen adapter along with the drying tube packed
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Purpose: To find out the densities and to find out the name of the unknown metals. (Based on the extensive and intensive properties) Check up the words mass‚ volume‚ density‚ extensive properties‚ and intensive properties. Where do the units for mass and volume) come from and what do they mean? What is the density of distilled water? What is Archimedes principle? Does temperature affect the density of a solid? Liquid? Gas? Materials: Safety glasses‚ 10‚ 25 or 50 mL graduated cylinders
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through the body through the plasma protein albumin which is essential in carrying hormones like fatty acids‚ this helps the endocrine glands hormones to reach its target cells. The Regulation of blood in the body is very important as it acts as a pH buffer by maintaining body temperature‚the blood dispenses heat around the body through the arteries and blood vessels‚ blood also helps to keep the cells and tissues of the body from losing their water content‚ the plasma in the blood is also very important
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bottom and about 0.5 cm of the top of the solution was clear. Table 4: The results of Gel Filtration Chromatography Eluate Component Volume collected in mL First Blue dextrin 3.00 Second Brown cytochrome 7.00 Third Clear phosphate buffer 5.00 Table 5: The result of Electrophoresis of Amino Acids Distance moved (+/-) cm Amino acid 10% Acetic acid Phosphate NH4OH Glycine -6.0 -3.2/+3.0 +9.0 Lysine -7.0 -2.0/0 +3.7 Glutamic
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During the preparation of the 0.100M Tris buffer‚ the calculated amount of ingredients brought the solution to a pH of 7.0‚ but the desired pH was 7.50. Discrepancies between the theoretically calculated amounts and the actual measured amounts of ingredients are likely to be the biggest source of error. Dilution affected our 0.0100M Tris buffer by decreasing its pH. The buffer was originally set to a pH of 7.48‚ but the pH gradually moved down by a pH unit of about 0.1 after each dilution. This is
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IB SL Biology Lab Molecular Biology: Transformation and Electrophoresis Christina Qi 2/16/07 Aim: How can a plasmid be engineered to include a foreign piece of DNA and how does gel electrophoresis separate DNA molecules present in a mixture? Hypothesis: If the pGLO plasmid is inserted into competent Escherichia coli cells‚ then the transformed bacteria will be resistant to ampicillin and will glow green under UV light. If samples of DNA are cut using certain restriction
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Research Question How will the addition of different pH buffers to amylase affect the rate of starch digestion measured using starch and iodine? Introduction Amylase is an enzyme found in human saliva and pancreas. It is the digestive enzyme that is needed to breakdown starch molecules. Amylase must be kept at certain conditions to function at its optimum level. This experiment will explore the effect of pH (1‚ 4‚ 7‚ 10‚ and 14) on the function of amylase by using starch and iodine. Usually
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