TEXT BOOK OF BIOCHEMISTRY For 2nd year Medical Laboratory Technician Course (INTERMEDIATE VOCATIONAL) Author : S. Srinivasa Rao‚ B.Pharmacy‚ Jr. Lecturer in M.L.T.‚ Alluri Sri Rama Raju Govt. Junior College‚ Shanthi Nagar‚ Khammam. Editor : S. Kamalakar Rao‚ M. Pharmacy Senior Lecturer in Pharmacy‚ Department of Biotechnology‚ Govt. institute for post diploma courses in engineering and technology‚ Ramanthapur‚Hyderabad. INDEX Chapter I. Page No. Instrumental methods of Biochemical
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After I selected my fish‚ I noticed that the tank began to grow algae. The next time I selected fish and invertebrates‚ I chose to add cleaner shrimp and snails to my tank. The pH then started to go up‚ then down‚ and then up again so I added pH buffer solution so the pH could stabilize at 8.1. After that‚ I decided to add water to keep the specific gravity from getting too high and maintaining stable. 3. What problems‚ if any‚ did you have with any of the fish or invertebrates in the
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African Diaspora and the World After reading the short story “Diaspora” by Joanne Hyppolite‚ I got a vivid understanding of how diaspora can have an effect on one’s identity. Over the past month we have studied many theories and concepts concerning race‚ gender‚ and politics. There are three theories that capture the essence of Joanne Hyppolite’s worldview as a Haitian growing up in America: intersectionality‚ identity‚ and diaspora. Individuals oftentimes experience the theory of Intersectionality
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proven that pH buffer 7 is the optimum pH for catalase in potatoes. This is because the height of the bubbles was the highest of all three tests (pH 3‚ pH 7‚ pH 9) reaching an average height of 0.433 cm. See graph 3. While pH buffer 3’s average height was 0.233 cm only reaching a maximum height of 0.3 cm in Trial 3 and pH buffer 9’s average height being 0.333 cm only reaching a maximum height of 0.4 cm again in Trial 3. See table 1. Therefore this supports my hypothesis: At pH buffer 7 the enzyme
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The starting material for this lab was the dialyzed sample (stored at -20ᵒ C) from the previous lab. The CM sephadex resin (taken in a 50 mL tube) was already made swollen using Buffer C (20 mM HEPES‚ pH 7.9; 1 mM EDTA; 50 mM KCl). The dialyzed sample was thawed to the room temperature and gently poured over the resin. The tube was capped and kept on a rocker at room temperature for 1 hour. The tube was then centrifuged in a HS-4 rotor at 2500 rpm (1200g) for 5 minutes at 4ᵒ C. Supernatant was discarded
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Once the buffer had mixed with the substrate we removed 500 microliters of this new solution and added it to the "Start" cuvette. Using a clean tip‚ we pipetted 1 mL of enzyme into the 15 mL "Enzyme Reaction" conical tube‚ gently mixed‚ and then started our timer
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General College Chemistry LabPaq / Published by: Hands-On Labs‚ Inc. sales@HOLscience.com / www.HOLscience.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of General College Chemistry 50-0123-CK-02 LabPaq® is a registered trademark of Hands-On Labs‚ Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs‚ Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s
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basic. Any number lower is acidic. In this lab we have tested a number of substances on the pH scale and their effects when being poured on different colors of litmus paper. You can neutralize a base and an acid by combining the right amount between them. In the term H3O+ + OH- --> 2H2O you can see it neutralized. Just like in math if you add a negative to a positive they can neutralize each other out if it’s the same number or amount on both sides. In this lab we saw the effects of putting these substances
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Treatment of Results 1) What is the role of 0.25M sucrose as the medium for the fractionation process? Cold sucrose does not chemically react with cell organelles Due to the density and size of sucrose molecules‚ it is able to suspend pellets for configuration while providing a solution where the centrifugation can be better balanced Sucrose offers a liquid medium in which less dense fractions can be poured off as supernatant at the end of each centrifugation step. 0.25M sucrose solution
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dark reactions affect the rate of photosynthesis in chloroplasts. We will also be using a reference solution made of water‚ phosphate buffer‚ and active chloroplasts. The purpose of this solution will be used to set the transmittance level for the experiment. The control solution‚ which is different than the reference solution‚ is comprised of water‚ phosphate buffer‚ and DPIP. It will be
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