University of Texas at Tyler Lab 3C: Purification of L-Lactate Dehydrogenase By Affinity Chromatography on Cibacron-Blue Sepharose David Alexander 10-15-2014 Dr. Black Chem 4135.001 Abstract: Like the previous experiments‚ the ultimate goal of this lab was to purify the enzyme sample. However‚ this is the last lab for purification and high level techniques of purification were employed to achieve this. Dialysis was used first‚ lowering the small-molecule concentration within the sample. Finally
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Lawrence Joy Pengda Jin Jing Shang Hanish Mandru 1. What are the various types of malware? How do worms differ from viruses? Do Trojan horses carry viruses or worms? a. The various types of malware are viruses‚ worms‚ Trojan horses‚ polymorphic threats‚ virus and worm hoaxes‚ back door or trap door‚ denialofservice and distributed denialofservice‚ and mail bomb. b. A virus must be executed such as opening an infected email attachment while a worm can be initiated with or without the user downloading or executing the
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Gel Electrophoresis Lab SBI4U1 May 13th‚ 2013 Gel Electrophoresis Lab Purpose: The purpose of this lab is to learn how restriction enzymes cut DNA molecules at specific sequences‚ thus producing DNA fragments of various lengths. Students learn how fragments form unique patterns‚ which help to distinguish the base for DNA identification. This lab answers the question “whose DNA was left behind?”. Materials: * Transfer pipets * Agarose Gel * Dyed DNA samples *
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catalyzes the substrate molecule into a product. Enzymes have the capability to break or build compounds which keep cell systems functioning. For example‚ our digestive tract has catabolic enzymes which break apart food for storage and reuse. Our lab experiment studied the relationship of benzoquinone production when altering the pH and temperature of the reaction‚ as well as enzyme and substrate concentrations. We used a Spec 20 to evaluate absorbance at 540 nm. Absorbance represents rate of
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1. Why are spindle fibers important for mitosis? (5 points) Spindle fibers work as a path for chromosomes to travel(Campbell‚ 2009). 2. State the four bases that make up DNA. (4 points) a. (A)-Adenine b. (G)-Guanine c. (T)-Thymine d. (C)-Cytosine (Campbell‚ 2009). 3. What are the two base pairs? (2 points) a. AG b. CT 4. Answer the following questions: a. Define the term crossing over. (3 points) The phase in meiosis where chromosomes exchange segments of genetic materials (Campbell‚ 2009). b. Explain
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AP Bio Block B Photosynthesis Lab 12/6/12 Introduction: Photosynthesis is affected by light intensity‚ water‚ and temperature. Plants grow more abundantly because the weather is warm. Carbon Dioxide given off by animals is consumed by plants that replace the oxygen animals take it. Experimentation will help understanding how plants are vital because of the oxygen they release. If leaf disks in the experiment release oxygen‚ they will undergo photosynthesis and float. If
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Strawberry DNA lab Extraction Purpose: The purpose of this lab experiment is to see if this particular type of fruit which in this case is a strawberry contains DNA. Using materials such as a Ziploc bag to help keep the entire strawberry in one place while it gets smashed and test tube to help better see the DNA and break down of the liquid in the left over strawberry sediments. Background information: The long thick fibers of DNA store the information for the function of the chemistry
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Week 2 Lab #4 Assessment ISSC362: IT Security: Attack & Defense 20 december 2013 Lab #4 – Assessment Worksheet 1. Phase 1—Reconnaissance‚ Phase 2—Scanning‚ Phase 3—Gaining Access‚ Phase 4—Maintaining Access‚ Phase 5—Covering Tracks 2. Zenmap does an intense scan of all TCP ports in order to find open and vulnerable ports. 3. Phase 1 Reconnaissance 4. This is referred to by many names‚ but typically just called a vulnerability scan. Can be done with tools such as Nessus or Microsoft baseline
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The purpose of lab number thirteen was to examine the response of the daphnia to a chemical stimulant. Also examined was how human resting heart rates differed from the daphnia’s. Stimulants are any substances that raise the levels of activity in the body. Stimulants could be a number of things such as physical or chemical. Physical stimulus usually deal with something that affects the five senses such as sight‚ hearing these could be attributed to loud noises or too much light going into your eye
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Abby Goldschmidt Honors Biology 2° Mrs. Gempel September 3‚ 2015 Daphnia Lab Results Paper Abstract The goal of the study was to observe the effects of multiple chemicals on a Daphnia magna’s heart-rate compared to a control (pond water). The different chemicals were caffeine and alcohol. The heart-rate was the main variable in this experiment. The Daphnia’s heart-rate was observed for 15 seconds and then multiplied by 4 to show its heart-rate in one minute. This was repeated 4 times for each
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