Investigating the Activity of Cholinesterase and its Inhibitors Introduction: Enzymes are highly specific biological catalysts (proteins) that influence the rate of metabolic reactions (Smith‚ 1997). They are responsible for efficiently converting substrate molecules into products required to sustain life (Grisham et al.‚ 1999). The activity of enzymes can often be effected by other endogenous or foreign factors (e.g. inhibitors). Inhibitors are able to decrease the rate of enzymatic activity
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enzyme Inhibitors Irreversible enzymes are described as enzymes that require chemical activation. After chemical activation‚ the chemical reaction occurs between the target enzyme and the inhibitor. The chemical reaction results in the irreversible inhibition of the enzyme. In short‚ the enzyme catalyzes its own inactivation(Rando‚ 1974a). The purpose of irreversible inhibitors is to modifying key amino acid residues required for enzymatic activity. There are two types of irreversible inhibitors‚ which
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Acetylcholinesterase and Butyrylcholinesterase substrate selectivity and various acting cholinesterase inhibitors Introduction Cholinesterases are a group of enzymes present in mammals which breakdown certain neurotransmitters by hydrolyzing the ester bonds within a molecule (Rang & Dale‚ 2007). There are two major types of enzymes‚ acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Though similar in structure‚ they differ in distribution‚ function and substrate specificity. AChE is
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An acetylcholinesterase inhibitor has a toxic effect on the biological system. Specifically‚ cholinesterase inhibition alters homeostasis by blocking the enzyme cholinesterase‚ causing a disruption in the breakdown of the neurotransmitter‚ acetylcholine. The accumulation of acetylcholine and the concentration gradient of the toxin are factors that affect the range of possible symptoms. Mild poisonings include nausea‚ blurred vision‚ vomiting‚ and salivation. Paralysis and chest discomfort are moderate
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DNA synthesis inhibitors Nucleic acid inhibitors are chemicals which inhibit the production of nucleic acids including both DNA and RNA. DNA and RNA inhibitors inhibit enzyme action in DNA replication in the same way as the topoisomerase inhibitors we discussed earlier. Topoisomerase inhibitors (1) (10) are chemicals which interfere with the enzymes that allow DNA strands to separate and to re-join‚ a process that is required for the division of bacteria and without which DNA cannot effectively
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CHM1032L pre/post lab instructions Preparation is a key to success in this lab. For this reason‚ you are required to thoroughly read through the experiment information presented in the lab manual‚ and complete a pre-lab for each experiment you do. The prelab must be completed prior to the day of the experiment. Each Friday I will ask to see your completed prelab before I allow you to enter the lab. If you have not finished the pre-lab‚ I will not allow you to enter the lab and you will receive
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Lab report is one way we used during of after an experiment in a laboratory to subtly record and discuss the experiment. During a lab‚ we sometimes can observe only the physical part of the experiment‚ or may be some visible chemical changes. These changes indicate that the experiment we do is successful or not. However‚ in order to understand and achieve more from just simply doing the experiment‚ we write lab report to more profoundly understand the internal meanings of the experiment we do‚ and
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Chem 105 Guide to the Formal Laboratory Report The purpose of a formal report is to communicate effectively to another person the goal‚ procedure‚ data analysis method‚ and results of your laboratory work. The report is divided into several well-defined sections. Each section must be present in a complete report. To earn an outcome point for the laboratory report‚ a student must submit a formal lab report that earns a score of at least 90/100. Each error (factual‚ grammatical‚ typographical
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Criteria 6 8 10 Title Your lab report has a Title that is directly related to the lab experiment/ exercise. Your group or class must be able to come up with your own title You must make your own title based on scientific theory Aim You have clearly stated your aim Your aim is relevant and informative You must make your own aim based on scientific theory Background background information relevant to the lab experiment/ exercise You must be able to link your aim to the background The
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Institute Physics Course 2014 - Rocket Lab Report Title: RockSim and Electronic Altimeter Measurement Techniques Name: Scarlet Henriquez Email: scarletpatricia94@aol.com LAB SESSION ___8____Assignment _____7_____ Due Date: ___7/8/14______ Objective: Launch a smaller rocket to learn how to compare RockSim predictions (computer based) to an actual instrument measurement carried by the rocket (Electronic Altimeter). This Lab shows how better the electronic measurement
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