Objective: The main objective for this lab was to use the Bradford and DC Protein Assay in order to determine our concentration of E. Coli BL21(DE3) lysate. Principle of Methods: To accomplish this objective‚ each pair of lab partners received an E. Coli lysate sample which we had to thaw by warming it with our hands and centrifuge it. We extracted a total of 12 samples with known concentrations of bovine serum albumin and 10 samples of different amounts of lysate put into them. Using the different
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Graduate Lab Lab 1 :- Introduction to LabVIEW LabVIEW (short for Laboratory Virtual Instrument Engineering Workbench) is a system-design platform and development environment for a visual programming language from National Instruments. It uses a graphical programming language called G to produce its code. LabVIEW is commonly used for data acquisition‚ instrument control‚ and industrial automation on a variety of platforms including Microsoft Windows‚ various versions of UNIX‚ Linux‚ and Mac
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Ten years ago our electric motor business was in real trouble. Low labor rates allowed the Eastern Bloc countries to sell standard motors at prices we were unable to match. We had become the high-cost producer in the industry. Consequently‚ we decided to change our strategy and become a specialty motor producer. Once we adopted our new strategy‚ we discovered that while our existing cost system was adequate for costing standard motors‚ it was giving us inaccurate information when we used it to
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The Ritz Carlton Hotel Company On October 11‚ 2000 The Ritz-Carlton was on the eve of opening Washington DC’s first luxury hotel in 10 years. The 300 room hotel was part of a $225 million complex owned by Millennium Partners. This would be the first hotel opened of a six hotel deal between Millennium Partners and The Ritz-Carlton. Millennium Partners owned the properties and The Ritz-Carlton was hired to operate the hotels. After only seven days of training the 400 person staff would open the
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EVENT SAFETY RISK ASSESSMENT AND EVENT MANAGEMENT PLAN For small to medium sized community events EVENT SAFETY AND PLANNING As part of any good planning process hazards should be identified and risks assessed and controlled to minimise the potential for injury or harm. Events vary in size‚ nature and type‚ but all events require assessment‚ control and monitoring of risks. Attached is a publication produced by Safework SA which provides guidance in the type of risk assessment procedure
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Effects of Washington D.C Snipers Two men that decide they want to take people’s lives into their own hands‚ can change the way American citizens live their everyday lives. This exact situation happened over a twenty-three day period‚ when John Muhammad and John Malvo went on a shooting spree in Washington D.C. John Allen Muhammad‚ a forty-one year old veteran expert marksman of the Persian Gulf War‚ was the main culprit of the crime. He was accompanied by John Lee Malvo‚ a seventeen year old
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Doc Hendley‚ the writer and creator of Wine to Water‚ is an extraordinary human being. Like myself‚ he was unsure of his life‚ his purpose‚ and he spent a long while self-exploring to see what fit him best. He enjoyed the open road and the freedom he felt while relaxing with “wind therapy” in beautiful scenery. Ever since a young age‚ like Doc‚ when he was exploring the woods with his BB gun‚ I was exploring the woods on a four wheeler or ripping waves on a jetski. While he never really fit in with
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Induction Motor Drive K.Vijaya Bhaskar Reddy* ‚ Research Scholar‚ Andhra University‚ waltair‚ A.P‚ India vijayabhaskarareddy.k@bvrit.ac.in‚ G.V. Siva Krishna Rao‚ Professor‚ Dept. of Electrical Engineering‚ Andhra University waltair‚ A.P‚ India‚ gvskrishna_rao@yahoo.com T.S.Surendra ‚Principal‚Dept of Electrical and Electronics Engineering‚BVRIT‚Narsapur‚Medak‚AP‚India‚surendra.ts@gmail.com Abstract- This paper deals with simulation of Space vector modulated inverter fed induction motor drive
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The Marketing Mix (The 4 P’s of Marketing) Marketing decisions generally fall into the following four controllable categories: • Product • Price • Place (distribution) • Promotion The term "marketing mix" became popularized after Neil H. Borden published his 1964 article‚ The Concept of the Marketing Mix. Borden began using the term in his teaching in the late 1940’s after James Culliton had described the marketing manager as a "mixer of ingredients". The ingredients in Borden’s marketing
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Ford operates in the US auto mkt‚ a huge‚ but not a high growth market. Growth in last 7 yrs – 1% US players losing share to foreign players. Firms consolidating. Targeting smaller segments – large no of models‚ low sales volume / model Focus on fuel efficiency‚ alternate fuels. All firms targeting US mkt. Chrysler merged with Daimler Benz‚ breaking off now. Focussed on clean diesel Power - BLUETEC GM focussing on hybrid vehicles‚ tying up with Toyota. Toyota caught up with Ford in sales volume
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