chromatography Presented by – Miss. Shruti Vilas Kharat. SYBSc / FS13164 History- Paper Chromatography (PC) was first introduced by German scientist Christian Friedrich Schonbein (1865). PC is considered to be the simplest and most widely used of the chromatographic techniques because of its applicability to isolation‚ identification and quantitative determination of organic and inorganic compounds. Definition- Paper chromatography is an analytical method technique for separating and identifying mixtures
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Lab 3: Solubility of Organic Compounds Objectives: Understanding the relative solubility of organic compounds in various solvents. Exploration of the effect of polar groups on a nonpolar hydrocarbon skeleton. Introduction: The solubility of a solute (a dissolved substance) in a solvent (the dissolving medium) is the most important chemical principle underlying three major techniques you will study in the organic chemistry laboratory: crystallization‚ extraction‚ and chromatography.
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less matrix disturbances. Chromatography is the method most used for commercial sugar separations. Such separations are batch processes and normally imply expensive installations‚ low productivity and low yields of the desired product. Gas chromatographic analysis of mono and disaccharides requires conversion of sugars into their volatile derivatives. Conversion of sugars into alditol acetates and trimethylsilyl (TMS) ethers have been the most widely used methods for GC analysis of sugars. "Alditol
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explicitly identify the phase of matter of the mobile phase. The term “paper chromatography” used in this experiment’s title identifies the composition of the stationary phase. The compositions of the stationary and mobile phases define a specific chromatographic method. Indeed‚ many different combinations are possible. However‚ all of the methods are based on the rate at which the analyzed substances migrate while in simultaneous contact with the stationary and mobile phases. The relative affinity of
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Lesson Learning Outcomes Upon completion of this lecture‚ students should be able to: understand the structures and properties of amino acids know the groups of amino acid What are amino acids? Amino acids are molecules that when combined with each other proteins. Amino acids contain a central tetrahedral carbon atom (α-carbon) amine group‚ carboxyl group‚ R-side chain The R-side chain determines the different amino acids There are 20 common amino acids Amino acids can join via peptide
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ESTIMATION OF 1-4‚ DIOXANE IN SHAMPOOS/ CONDITIONERS/HAIR CREAMS/ SODIUM LAURETH SULFATE BY HEAD SPACE GAS CHROMATOGRAPHIC TECHNIQUE INTRODUCTION 1-4‚ DIOXANE 1‚4-Dioxane is a heterocyclic organic compound‚ classified as an ether. It is a colorless liquid with a faint sweet odor similar to that of diethyl ether. The compound is often called simply Dioxane because the other Dioxane isomers (1‚2- and 1‚3-) are rarely encountered. CHEMISTRY OF 1-4‚ DIOXANE
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heating it. • Always wear department approved eye goggles at all times. • Long pants and closed toe shoes are required. Table of Reagents Reagent Mol. Wt. (g/mol) Bp ( ͦ C) Mp ( ͦ C) Density (g/ml) Acetanilide 135.17 304 113-115 1.219 Fluorene
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sodiumsulfate and evaporating it to dryness. The percentage yield was computed by getting theratio of the weight of the crude caffeine (residue) and weight of the tea leaves used. Thepercentage yield was 0.92. INTRODUCTION Extraction is a method of seperation in which a solid or solution is contacted with a liquid solvent (the two being mutually insoluble) to transfer one or more components into to the solvent. There are two main types of extraction in chemistry‚ namely: Liquid-liquid extraction or solvent
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Variables Affecting the Dissolution Profile of Zolmitriptan Liquisolid OrodispersibleTablets(ODTs) Different formulas were prepared (F1-F18) using different variables to reach the best formula. Each formula was evaluated to study the pre and post compression parameters. Angle of repose‚ carr’s index and Hausner’s ratio as a pre-compression tests for powder flow properties as well as hardness‚ friability‚ wetting time‚ content uniformity and in vitro disintegration time as a post-compression tests
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used to model a reservoir fluid. The fluid is a combined gas and oil stream. Learning Objectives Once you have completed this section‚ you will be able to: • • Understand the steps required for oil characterization. Characterize an oil using chromatographic data. Prerequisites Before beginning this module you need to understand the basics of the fluid package (Getting Started). 2 Oil Characterization 3 Process Overview Residue C6+ Component Mole Fraction Mass Fraction Volume Fraction
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