hair blower Allow solvent to dry and dab another extract Dab extract onto chromatographic paper‚ along 4mm of starting line‚ using capillary tube Draw two lines: (1) starting line 1.5cm above V point‚ (2) finish line 1cm below other end Cut strip of chromatographic paper (1cmx10cm)‚ cut one end into Vshaped edge Repeat dabbing 58times to build up concentrated spot Put 2mL chromatographic solvent into 6" test tube Bend paper clip‚ attach to cork stopper; attach
Premium Central nervous system Nervous system Water
chromatography? 7 8 9 10 _______________________________________ d) The first thing that is common among all chromatographic procedures. 5 6 7 8 _______________________________________ _________________________________________________ 7)Fortunately‚ all the different procedures of chromatography have two major things in common. 8)The first thing that is common among all chromatographic procedures is the structure of the laboratory set – up. 9)The structures will always have two parts. 10)These
Premium Chromatography Mixture Gas chromatography
There are a number of themes that can be extracted from the play based film‚ "Becket." They range everywhere from loyalty‚ honor‚ and friendship to politics and religious views of the time and concepts of secular and spiritual seperations. Many of these concepts presented in the film hold much contemporary relavance. What seems to be the most prevalent theme in "Becket" is honor. When looking into the film‚ we are made to ask the question "what is honor and who through the film has it?" Acording
Premium Canterbury
NATIONAL UNIVERSITY of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure
Premium Chromatography High performance liquid chromatography Protein
1 This essay will examine the role of attachment in childhood and it’s subsequent formation of relationships. Most babies of mammals exhibit the same patterns as human infants; they seek proximity to the mother and react with anxeity on seperation from her‚ which is the essense pf attachment behaviour. John bowlby believed that attachment was an innate pattern and it helped infants to survive. Bowlby had observed how baby monkeys reacted with distress on sepearation from their mother for
Premium Attachment theory Developmental psychology Psychology
A simple‚ specific‚ accurate and rapid reversed phase high performance liquid chromatographic method was developed for the simultaneous estimation of cilnidipine and chlorthalidone‚ using a Hiber® C18 (25cm x 4.6 mm)‚ Lichrospher® 100‚ RP-18 e (5µm) column and a mobile phase composed of phosphate buffer(pH 4.0 adjusted with orthophosphoric acid) : Acetonitrile (30:70‚ v/v). The retention times of Cilnidipine and Chlorthalidone were found to be 3.967 min and 6.427 min‚ respectively. Linearity was
Premium High performance liquid chromatography Chromatography Solubility
Gas chromatography is the common method used for the biogasoline analysis. Biogasoline catalytic cracking process requires detailed qualitative and quantitative analysis of the hydrocarbon composition of its oil and fraction. In this analysis‚ their fractional composition‚ saturated vapor pressure‚ density and antiknock rating will be investigated. By knowing the qualitative and quantitative composition of the gasolines‚ the content of n-paraffin‚ isoparaffin‚ aromatic‚ naphthene‚ and olefin (PIANO)
Premium Chromatography Gas chromatography Vapor pressure
References: Fessenden‚ J.‚ & Feist‚ P. (2001). Organic laboratory techniques. Australia: Brooks/Cole; pgs. 119-125‚ 133-138. Robards‚ K. (1994). Principles and practice of modern chromatographic methods. San Diego‚ California: Academic Press‚ Inc.; pgs. 1-34‚36-225. Williams‚ T. (1946). An Introduction to chromatography. London: Blackie & Son Limited; pgs. 22-34‚ 47-50. http://curlyarrow.blogspot.com/2011/11/lets-talk-about-tlcs-part-5-iodine
Free Chromatography Thin layer chromatography
.......................................... 409 16.2.3 Separation of Flavonoid Aglycones and Glycosides on Silica Gel and Reversed-Phase TLC....................................... 410 16.2.4 Use of Mathematical Methods for the Optimization of Chromatographic Conditions for Flavonoid Separations ........ 413 16.2.5 Multidimensional Planar Chromatography (Unidimensional Multiple Development and Two-Dimensional Development)..... 415 16.2.6 Quantitative Evaluation.....................................
Free Chromatography
is hard to distinguish the similar compounds because only one solvent is used unlike in 2-dimensional where in 2 solvents are used to separate the compounds. 3. What are the considerations in choosing a chromatographic solvent? - One of the considerations of a chromatographic solvent is whether it will bring out different Rf values of the samples used. If all of the compounds have the same Rf values then the separation of pigments would have failed due to the fact that all of them are
Free Chromatography Thin layer chromatography Analytical chemistry