Experiment 5 Title : Thin Layer Chromatography Objectives: i. To distinguish polar and non-polar solvents. ii. To familiar with the analysis technique by using the thin layer chromatography. iii. To differentiate the retention factor‚ Rf for different compounds. [pic] Result: |Compound |Distance traveled by the compound | |o-nitroanaline |2.45
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unknown compound. In this experiment‚ chromatography and titration were performed to identify the unknown amino acid. Within experimental error‚ the results were consistent with the reference literature cited in this report. Experimental Thin Layer Chromatography The amino acid standards used in this experiment were Alanine‚ Glycine‚ Serine‚ and Histidine. These standards and the unknown were separated by performing a method of chromatography. Thin layer chromatography (TLC) was performed by using a mobile
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Turning A New Leaf 1-turning a new leaf is a phrase used to show that a person has changed by starting fresh. This applies to Dick because he "turned a new leaf" by getting a job and he stopped drinking. Julia turned a new leaf by becoming much more mature during the story. Julia lives up to the phrase because she actually gets more mature as opposed to Dick who sort of lost it at the end. I would say that Julia is the protagonist in the story. 5- In the story Phil is in love with Julia
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Size Exclusion Chromatography Size Exclusion Chromatography (SEC) is the separation technique based on the molecular size of the components. Size exclusion chromatography is a kind of method to separate different size of molecules that put in solution. It was first discovered by two scientists who named Grant Henry Lathe and Colin R Ruthven. Both of them received the John Scott Award for this fabulous invention. There are various applications for Size exclusion chromatography such as biochemical
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usually found in spinach leaves: Carotene (yellow)‚ Phaeophytin (yellow-grey)‚ Xanthophyll (yellow-brown)‚ Chlorophyll a (blue-green)‚ Chlorophyll b(green). Photosynthetic pigment is located in the chloroplast of the leaf. The function of chlorophyll a and chlorophyll b is to trap red and blue violet light when they carry out photosynthesis‚ chlorophyll a occurs in green plants while chlorophyll b occurs in green algae.Carotene and Xanthophyll are carotenoid where it can protect the human eye from
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significant difference in Daphnia’s response when exposed to red light compared to white light. Thus‚ the null hypothesis was rejected and the experimental hypothesis was accepted. These findings could be due to the negative phototactic response that occurs when they are exposed to visible light that are above 600 Mn (nanometers) that are also considered the color red. This shows that Daphnia have a greater phototactic reaction to red light than when exposed to white light. When compared to a similar
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CHM 510 LABORATORY REPORT Experiment 1: Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature Name: AFIQ B. ANWAR Student No.: 2012621072 (AS2253A) Date of experiment: Date of report submission: Lecturer’s Name: PN. HALIZA Gas Chromatography (GC): Optimization of Flow Rate and Column Temperature INTRODUCTION The main purpose of the experiment is to investigate the effects of column temperature and flow rate on the separation of methyl esters compounds
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able to experimentally analyze and isolate amino acids by separation techniques. One such method of separation is thin layer chromatography (TLC). In this method‚ a silica coated aluminum plate is spotted with the solution being separated‚ and the plate is placed in a developing jar containing a small amount of solvent. With time‚ the solvent
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Low-Carb Lettuce and Tuna Salad Ingredients: • 1 ½ cups canned tuna in water‚ drained • ½ romaine lettuce‚ finely chopped • 2 teaspoons Dijon mustard • ½ cup Greek yoghurt • 1 tablespoon lemon juice • 2 tablespoons sour cream • Dash of sea salt Directions: 1. Place the tuna in a bowl and flake it with a fork. Mix in lettuce‚ mustard‚ yoghurt‚ sour cream‚ lemon juice and salt. Toss the salad then place it in the fridge for 1 hour. 2. Transfer the salad to smaller bowls and serve cold. 3. This
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Last leaf is a short story that entails the treasury of life and the existence of faith and hope. It heeds to the importance of living and how we deal with the hindrances we battled through our life story. It is a moving story across the traps that come across us in the most significant part of our lives. The value of life is the centerpiece of the story where all things go back and revolve. A part of the story gives us a hint that God is the only one who knows whether we ride on with life and chances
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