Materials and methods Preparation of saponins from sea cucumber: Saponins were isolated from the sea cucumber‚ Holothuria thomasi (purchased from Hurghada‚ Red sea‚ Egypt and identified by Zoology department‚ Faculty of science‚ Tanta‚ Egypt)‚ according to the method described by Hu et al.‚ (2010) with some modification. Air-dried body walls (750 g) of sea cucumber were grinded into powder and extracted with eight liter with refluxing ethanol. The combined extracts were evaporated with rotary evaporator
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OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to‚ during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules‚ and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous
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5.05 Mixtures and solutions Lab report Ink Chromatography Chromatography is also used to compare and describe chemical substances. The chromatographic sequence of sorbed substances is related to their atomic and molecular structures. A change in a chemical substance produced by a chemical or biological reaction often alters the solubility and migration rate. With this knowledge‚ alterations or changes can be detected in the substance. Chromatography serves mainly as a tool for the examination and
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Chromatographic Separation of Sodium Fluorescein and Methylene Blue In this two-part lab the students will: • Learn the techniques associated with thin layer and column chromatography. • Determine the effects of the stationary phase in separating a mixture by chromatography. • Determine the effects of the mobile phase in separating a mixture by chromatography. • Conduct a separation using column chromatography. In the first week‚ the students will determine the best solvent system for separating
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Hormone Control of Leaf Abscission Lab Report February 24‚ 2011 By Patrick Mills Introduction- Hormones are a chemical are transported within an organism and are highly specific chemical signals between cells. The hormones we used were lanolin‚ auxins(IAA)‚ cytokinins(CYT)‚ ethylene(ETH)‚ and gibberellins(GA) and each has a different purpose. Auxins will control cell elongation‚ cytokinins promote the act of cell division‚ ethylene causes the ripening of fruit‚ and gibberellins will elongate
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How Chromatography Helps The Human Race Chromatography is the separation of a mixture by passing it in solution or suspension or as a vapor (as in gas chromatography) through a medium in which the components move at different rates. “Chromatography is done by making of a mixture move past the solids‚ or across the surface of a solid‚ like paper. The mixture is poured onto a solid surface. As the different components of the liquid run down the solid‚ some of them move more slowly than other. A component
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Introduction Chromatography is a technique used to separate a mixture into its individual components. In this experiment‚ chromatography is done by using a thin layer chromatography (TLC) plate and filter paper. The word ‘chromatography’ itself means the separation of colours. However‚ nowadays‚ both coloured and colourless compound are used in chromatography by various methods. In chromatography‚ the separation process of mixture is mainly based upon the polarity and molecular weight of sample
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Column Chromatography of Plant Pigments Paul Ibarbia‚ Gene Paolo Jasmin‚ Gianpaolo Jimenez and Lorenzo Labicane* Department of Biology‚ University of Santo Tomas‚ Manila‚ Philippines Abstract Column chromatography of plant pigments is the separation of plant pigments extracted from Capsicum frutescens (siling labuyo). No slurry is prepared of the eluent; eluent with the stationary phase is set and then carefully poured into the column. The eluent are the solvents which are responsible for the
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Analysis of Food Colorings by Paper Chromatography Introduction Paper Chromatography one method for testing the purity of compounds and identifying substances. This is a useful technique because it is relatively quick and requires small quantities of material. Separations in paper chromatography involve the same principles as those in thin layer chromatography. The substances are distributed between a stationary phase and a mobile phase. The stationary phase is usually
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The overall purpose of the lab is the understanding of the process and goals of the scientific method. This lab illustrates that this method is very useful to answer a question or address a problem base on observations and the available information. Hypothesis: There are more red marbles than black marbles. We were able to address our hypothesis with the data collected because 26.32% of the marbles are red and just 14.47% are black. Also‚ the percentage of red marbles is the biggest in comparison
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