Candy Chromatography: What makes those colors? The point of doing this project is to use paper chromatography to see which dyes are used in the coatings of your favorite colored candies. Chromatography is a technique for separating mixtures. Chromatography can be used to separate specific components from a complex mixture‚ based on molecular size or other chemical properties. It can also be used to identify chemicals‚ for example crime scene samples like blood‚ drugs‚ or explosive residue. Highly
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mobile phase can be liquid‚ gas‚ or supercritical fluid. How Does Chromatography Work? Chromatography is a surface effect. It works by first starting off with an unidentified liquid being injected into a column. As the chemicals in the liquid move down the column‚ they separate depending on their physical and chemical attributes‚ such as speed and weight. To easily picture the process‚ the example will pertain to a paper chromatography process in which the liquid is moving over the paper‚ separating
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Gas Chromatography: Purifying alcohol Introduction The goal of this lab is to understand the principles of chromatography by purifying alcohol using fractional distillation. Running standards with gas chromatography we were able to see and calibrate our data to find not only how much ethanol our alcohol attained but also what a mixed unknown sample contained. Chromatography is a way of being able to separate substances in solution that can help not only identify the analytes (the studied
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Title: Thin-Layer Chromatography Objectives: Part1: 1. To learn the technique of TLC and the visualization of colourless components. 2. To identify an unknown drug by a TLC comparison with standard compounds. Part 2: To learn the separation technique by using Thin Layer Chromatography plate in separating a mixture of compounds into individual pure compound by using Spinach Leaf. Introduction: (i) General Concepts Chromatography is a common and powerful method used to separate and analyze complex
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Background Information and Research Paper chromatography uses selective adsorption on a strip of paper. It is used for separating and identifying mixtures that are or can be colored‚ especially pigments. This can also be used in secondary or primary colors in ink experiments. Is it used for Contaminants in rainwater‚ Analysis of narcotics‚ and Detection of substances in urine. Purpose • The purpose of this lab is to see the separation of dye on the candy. Data and Observations Marker Color
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Chromatography Analysis of Black Pens to Determine Unknown Sample Purpose: Paper chromatography was performed on five different black pens‚ using four different solutions to determine which would be most appropriate to use on an unknown sample. Paper chromatography was then performed on the unknown sample to identify which pen was used to create it. Procedure: Each person participating in the analysis was assigned one solution to work with: V. Temple used distilled water‚ D. Sellers used
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Instructor: Ma ’am Lioanag Section: Civil Engineering 1-19 Date Performed: July 27 ‚ 2013 Group No.: 3 Date Submitted: August 3 ‚ 2013 Experiment No. 4 : PAPER CHROMATOGRAPHY INTRODUCTION The objective of this experiment was to resolve a given sample into its components by paper chromatography‚ to evaluate the retention factor of each component in the sample and to compare the relative solubility of the various components of a mixture of colors in a given solvent. METHODOLOGY
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Affinity chromatography technique is used to separate proteins found in a mixture of solution. Affinity chromatography uses the strong interaction between a given protein and its corresponding molecule. In today’s lab‚ affinity chromatography was used to purify L-lactate dehydrogenase‚ which contains histidine-tagged protein. The histidine- tagged protein forms a strong interaction with the Ni-NTA column due to the presence of nickel ions. Varying concentration of imidazole was added to the column
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CHEM 2204 Chromatography Lab by wyk.wong » Fri Jul 11‚ 2014 10:25 am Results and Calculations Rf values Rf=(Distance moved by the spot (cm))/(Distance moved by the solvent front (cm)) Toluene: Rf=2 cm/3.8 cm=0.53 (Fluorenone) Rf=1.1 cm/3.8 cm=0.29 (Fluorene) Hexane: Rf=1.8 cm/2.2 cm=0.82 (Fluorene) Rf=0 cm/2.2 cm=0 (Fluorene Table 1: Experimental IR peaks compared to literature IR peaks for fluorenone Functional group Experimental peak (cm-1) Literature peak (cm-1) C-H 3010.5 3013
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understand that because substances have different properties‚ such as mass‚ that can be separated by chromatography. In our experiment‚ we chose 4 different color source to separate in water. We made sure that all the sources were water soluble because only polar substances will dissolve in water. We chose a black wet-erase marker‚ a red marker‚ a green marker‚ and mixed food coloring to test. The chromatography paper was split into 4 sections‚ about 2 cm above the bottom edge and evenly spaced out. Each
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