________________________12345_______________________ Date ____23.8.2013___________ Block _______ Training Lab: Paper Chromatography I. Background Information: When working in a lab‚ scientists often need to identify different molecules that are present in a sample they are studying. There are many ways to identify unknown molecules/chemicals in a sample. The method you will be using today is called Paper Chromatography and consists of 2 steps. First‚ you will separate the unknown chemicals and then you will identify
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the Kool-Aid was correct. However‚ solely based upon the Rf values‚ the dyes in the green Kool-Aid are Red 40 and Yellow 6 as those are closet Rf value to the numeric data collected and calculated from the Kool-Aid chromatogram. However‚ the chromatography paper in both trials display that the dyes in Kool-Aid are a form of yellow and a form of blue because one color band was of a blue tint and the other‚ a yellow tint. Therefore‚ based on this qualitative data‚ the dyes in the green Kool-Aid are
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Separations: Chromatography of M&M and Ink Dyes Almost all substances we come into contact with on a daily basis are impure; that is‚ they are mixtures. Similarly‚ compounds synthesized in the chemical laboratory are rarely produced pure. As a result‚ a major focus of research in chemistry is designing methods of separating and identifying components of mixtures. Many separation methods rely on physical differences between the components of a mixture. For example‚ filtration takes advantage of substances
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Chromatography refers to a set of laboratory methods used in separating as well as purifying biomolecules. A variety of chromatography techniques exist‚ and all depend on the interaction between a stationary and a mobile state. Two types of chromatography methods were examined in this investigation. First‚ ion-exchange chromatography was used. This method separates ions and polar molecules based on their affinity to the ion exchanger [2]. Specifically‚ cation-exchange chromatography was performed
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Separation techniques LIQUID CHROMATOGRAPHY ‘THE ART OF SEPARATION’ CHROMATOGRAPHY – AN INTRODUCTION Chromatography is a technique through which a mixture of chemical components are separated‚ identified and determined accurately. This technique while provides a way for analytical separations‚ also useful for preparative techniques by which pure compounds can be obtained. Detector Signal Blue Compound Sample Injection + Mobile Phase Retention Time Red Compound It is i defined d fi d as a
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Performance Liquid Chromatography (HPLC) Instructor: Dr. Hüseyin BOZKURT High Performance Liquid Chromatography (HPLC) is one mode of chromatography‚ the most widely used analytical technique. Chromatographic processes can be defined as separation techniques involving mass-transfer between stationary and mobile phases. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high performance liquid chromatography (HPLC).
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Separation of Amino Acids by Cation Exchange Chromatography Introduction and Purpose: Amino acids are small biomolecules that have a carboxylic acid backbone in common‚ as well as an amino group attached to a saturated carbon. There are many amino acids‚ but there are 20 most commonly know amino acids. Amino acids are the fundamenta building blocks of other biomolecules like proteins and ezymes (Davidson‚ 2015). This experiment examined a mixture of 3 amino acids. The purpose of this experiment
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Question 1 1.1 Silica gel chromatography: This is known as the stationary phase in column chromatography. Firstly‚ the tapered exit of the column is sealed using porous material. This porous material serves as support for the packing material‚ and prevents it from exiting the pipe. Thereafter‚ silica gel is compacted into the glass pipe to make the separating column. In finishing preparation of the column‚ the solvent which is used as the mobile phase is then passed through the dry column. Then the
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between the Genotype and Phenotype of Drosophila melanogaster Eye Pigment Mariam Rahmanyar 100486882 T.A: Zahra Mortaji BIOL 2020 Results: Our TLC plate showed all colours expressed by the pteridine pigments for the wild-type Drosophila as expected (Figure 1). The sample A mutant which was a bright-red eye mutant also expressed the same pigments as our wild-type. Compared to the wild type‚ sample A pigment spots were lower intensity in colour under the UV light (Figure 2)
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Experiment 2: Dehydration of an Alcohol: Distillation and Gas Chromatography Preparation of Methylcyclohexenes Purpose: The basic purpose of this experiment is to carry out the dehydration of an alcohol and isolate the reaction products by distillation. Gas Chromatography will be utilized to analyze the reaction mixture. Table of Reagents: Compound (g) Molecular Weight (g/mol) Grams (g) Moles 6 mL of 2-methylcyclohexanol (C7H14O) 114.19 g/mol 6 mL x 0.943g = 5.66 g 1 mL 5.66 g x
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